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Genomic analysis of Asian honeybee populations in China reveals evolutionary relationships and adaptation to abiotic stress

The geographic and biological diversity of China has resulted in the differential adaptation of the eastern honeybee, Apis cerana, to these varied habitats. A. cerana were collected from 14 locations in China. Their genomes were sequenced, and nucleotide polymorphisms were identified at more than 9...

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Autores principales: Shi, Peng, Zhou, Jun, Song, Huali, Wu, Yujuan, Lan, Lan, Tang, Xiangyou, Ma, Zhengang, Vossbrinck, Charles R., Vossbrinck, Bettina, Zhou, Zeyang, Xu, Jinshan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7713975/
https://www.ncbi.nlm.nih.gov/pubmed/33304549
http://dx.doi.org/10.1002/ece3.6946
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author Shi, Peng
Zhou, Jun
Song, Huali
Wu, Yujuan
Lan, Lan
Tang, Xiangyou
Ma, Zhengang
Vossbrinck, Charles R.
Vossbrinck, Bettina
Zhou, Zeyang
Xu, Jinshan
author_facet Shi, Peng
Zhou, Jun
Song, Huali
Wu, Yujuan
Lan, Lan
Tang, Xiangyou
Ma, Zhengang
Vossbrinck, Charles R.
Vossbrinck, Bettina
Zhou, Zeyang
Xu, Jinshan
author_sort Shi, Peng
collection PubMed
description The geographic and biological diversity of China has resulted in the differential adaptation of the eastern honeybee, Apis cerana, to these varied habitats. A. cerana were collected from 14 locations in China. Their genomes were sequenced, and nucleotide polymorphisms were identified at more than 9 million sites. Both STRUCTURE and principal component analysis placed the bees into seven groups. Phylogenomic analysis groups the honeybees into many of the same clusters with high bootstrap values (91%–100%). Populations from Tibet and South Yunnan are sister taxa and together represent the earliest diverging lineage included in this study. We propose that the evolutionary origin of A. cerana in China was in the southern region of Yunnan Province and expanded from there into the southeastern regions and into the northeastern mountain regions. The Cold‐Temperate West Sichuan Plateau and Tropical Diannan populations were compared to identify genes under adaptive selection in these two habitats. Pathway enrichment analysis showing genes under selection, including the Hippo signaling pathway, GABAergic pathway, and trehalose‐phosphate synthase, indicates that most genes under selection pressure are involved in the process of signal transduction and energy metabolism. qRT‐PCR analysis reveals that one gene under selection, the AcVIAAT gene, involved in the GABAergic pathway, is responding to cold temperature stress. Through homologous recombination, we show that the AcVIAAT gene is able to replace the CNAG_01904 gene in the fungus Cryptococcus neoformans and that it makes the fungus less sensitive to conditions of oxidative stress and variations in temperature. Our results contribute to our understanding of the evolutionary origin of A. cerana in China and the molecular basis of environmental adaptation.
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spelling pubmed-77139752020-12-09 Genomic analysis of Asian honeybee populations in China reveals evolutionary relationships and adaptation to abiotic stress Shi, Peng Zhou, Jun Song, Huali Wu, Yujuan Lan, Lan Tang, Xiangyou Ma, Zhengang Vossbrinck, Charles R. Vossbrinck, Bettina Zhou, Zeyang Xu, Jinshan Ecol Evol Original Research The geographic and biological diversity of China has resulted in the differential adaptation of the eastern honeybee, Apis cerana, to these varied habitats. A. cerana were collected from 14 locations in China. Their genomes were sequenced, and nucleotide polymorphisms were identified at more than 9 million sites. Both STRUCTURE and principal component analysis placed the bees into seven groups. Phylogenomic analysis groups the honeybees into many of the same clusters with high bootstrap values (91%–100%). Populations from Tibet and South Yunnan are sister taxa and together represent the earliest diverging lineage included in this study. We propose that the evolutionary origin of A. cerana in China was in the southern region of Yunnan Province and expanded from there into the southeastern regions and into the northeastern mountain regions. The Cold‐Temperate West Sichuan Plateau and Tropical Diannan populations were compared to identify genes under adaptive selection in these two habitats. Pathway enrichment analysis showing genes under selection, including the Hippo signaling pathway, GABAergic pathway, and trehalose‐phosphate synthase, indicates that most genes under selection pressure are involved in the process of signal transduction and energy metabolism. qRT‐PCR analysis reveals that one gene under selection, the AcVIAAT gene, involved in the GABAergic pathway, is responding to cold temperature stress. Through homologous recombination, we show that the AcVIAAT gene is able to replace the CNAG_01904 gene in the fungus Cryptococcus neoformans and that it makes the fungus less sensitive to conditions of oxidative stress and variations in temperature. Our results contribute to our understanding of the evolutionary origin of A. cerana in China and the molecular basis of environmental adaptation. John Wiley and Sons Inc. 2020-11-02 /pmc/articles/PMC7713975/ /pubmed/33304549 http://dx.doi.org/10.1002/ece3.6946 Text en © 2020 The Authors. Ecology and Evolution published by John Wiley & Sons Ltd. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Research
Shi, Peng
Zhou, Jun
Song, Huali
Wu, Yujuan
Lan, Lan
Tang, Xiangyou
Ma, Zhengang
Vossbrinck, Charles R.
Vossbrinck, Bettina
Zhou, Zeyang
Xu, Jinshan
Genomic analysis of Asian honeybee populations in China reveals evolutionary relationships and adaptation to abiotic stress
title Genomic analysis of Asian honeybee populations in China reveals evolutionary relationships and adaptation to abiotic stress
title_full Genomic analysis of Asian honeybee populations in China reveals evolutionary relationships and adaptation to abiotic stress
title_fullStr Genomic analysis of Asian honeybee populations in China reveals evolutionary relationships and adaptation to abiotic stress
title_full_unstemmed Genomic analysis of Asian honeybee populations in China reveals evolutionary relationships and adaptation to abiotic stress
title_short Genomic analysis of Asian honeybee populations in China reveals evolutionary relationships and adaptation to abiotic stress
title_sort genomic analysis of asian honeybee populations in china reveals evolutionary relationships and adaptation to abiotic stress
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7713975/
https://www.ncbi.nlm.nih.gov/pubmed/33304549
http://dx.doi.org/10.1002/ece3.6946
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