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High-throughput glycomic analyses reveal unique oligosaccharide profiles of canine and feline milk samples

Oligosaccharides are important components of milk, serving as substrates for the intestinal microbiota, acting as antimicrobials that prevent pathogen colonization, and supporting the developing gastrointestinal immune system of neonates. Nutrient composition of canine and feline milk samples has be...

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Autores principales: Wrigglesworth, David J., Goonatilleke, Elisha, Haydock, Richard, Hughes, Kevin R., Lebrilla, Carlito B., Swanson, Kelly S., Jones, Paul, Watson, Phillip
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7714234/
https://www.ncbi.nlm.nih.gov/pubmed/33270789
http://dx.doi.org/10.1371/journal.pone.0243323
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author Wrigglesworth, David J.
Goonatilleke, Elisha
Haydock, Richard
Hughes, Kevin R.
Lebrilla, Carlito B.
Swanson, Kelly S.
Jones, Paul
Watson, Phillip
author_facet Wrigglesworth, David J.
Goonatilleke, Elisha
Haydock, Richard
Hughes, Kevin R.
Lebrilla, Carlito B.
Swanson, Kelly S.
Jones, Paul
Watson, Phillip
author_sort Wrigglesworth, David J.
collection PubMed
description Oligosaccharides are important components of milk, serving as substrates for the intestinal microbiota, acting as antimicrobials that prevent pathogen colonization, and supporting the developing gastrointestinal immune system of neonates. Nutrient composition of canine and feline milk samples has been described previously, but little is known about the oligosaccharide content. Therefore, the objective of this study was to characterize canine and feline milk samples using a high-throughput glycomics approach. 23 dogs (9 Labrador retriever and 14 Labrador retriever x golden retriever crossbreed) and 6 domestic shorthair cats were recruited to the study. Milk samples were collected by manual expression at time points after parturition. Samples were collected across 2 phases per species, differentiated by maternal diet. Following extraction, oligosaccharide content was determined by liquid chromatography-mass spectrometry (LC-MS). In canine milk samples, 3 structures accounted for over 90% of all oligosaccharides detected across two diet groups. These were 3’-sialyllactose, 6’-sialyllactose, and 2’-fucosyllactose. In feline samples, a more diverse range of oligosaccharides was detected, with up to 16 structures present at relative abundance >1% of the total. Difucosyllactose-N-hexaose b, 3’-sialyllactose and lacto-N-neohexaose were all detected at abundances >10% in feline milk samples. Statistically significant differences (p<0.05) in oligosaccharide abundances were observed between collection time points and between diet groups within species. These data explore the oligosaccharide content of canine and feline maternal milk, representing an opportunity to generate a fundamental understanding of the nutritional needs of new-born puppies and kittens.
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spelling pubmed-77142342020-12-09 High-throughput glycomic analyses reveal unique oligosaccharide profiles of canine and feline milk samples Wrigglesworth, David J. Goonatilleke, Elisha Haydock, Richard Hughes, Kevin R. Lebrilla, Carlito B. Swanson, Kelly S. Jones, Paul Watson, Phillip PLoS One Research Article Oligosaccharides are important components of milk, serving as substrates for the intestinal microbiota, acting as antimicrobials that prevent pathogen colonization, and supporting the developing gastrointestinal immune system of neonates. Nutrient composition of canine and feline milk samples has been described previously, but little is known about the oligosaccharide content. Therefore, the objective of this study was to characterize canine and feline milk samples using a high-throughput glycomics approach. 23 dogs (9 Labrador retriever and 14 Labrador retriever x golden retriever crossbreed) and 6 domestic shorthair cats were recruited to the study. Milk samples were collected by manual expression at time points after parturition. Samples were collected across 2 phases per species, differentiated by maternal diet. Following extraction, oligosaccharide content was determined by liquid chromatography-mass spectrometry (LC-MS). In canine milk samples, 3 structures accounted for over 90% of all oligosaccharides detected across two diet groups. These were 3’-sialyllactose, 6’-sialyllactose, and 2’-fucosyllactose. In feline samples, a more diverse range of oligosaccharides was detected, with up to 16 structures present at relative abundance >1% of the total. Difucosyllactose-N-hexaose b, 3’-sialyllactose and lacto-N-neohexaose were all detected at abundances >10% in feline milk samples. Statistically significant differences (p<0.05) in oligosaccharide abundances were observed between collection time points and between diet groups within species. These data explore the oligosaccharide content of canine and feline maternal milk, representing an opportunity to generate a fundamental understanding of the nutritional needs of new-born puppies and kittens. Public Library of Science 2020-12-03 /pmc/articles/PMC7714234/ /pubmed/33270789 http://dx.doi.org/10.1371/journal.pone.0243323 Text en © 2020 Wrigglesworth et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Wrigglesworth, David J.
Goonatilleke, Elisha
Haydock, Richard
Hughes, Kevin R.
Lebrilla, Carlito B.
Swanson, Kelly S.
Jones, Paul
Watson, Phillip
High-throughput glycomic analyses reveal unique oligosaccharide profiles of canine and feline milk samples
title High-throughput glycomic analyses reveal unique oligosaccharide profiles of canine and feline milk samples
title_full High-throughput glycomic analyses reveal unique oligosaccharide profiles of canine and feline milk samples
title_fullStr High-throughput glycomic analyses reveal unique oligosaccharide profiles of canine and feline milk samples
title_full_unstemmed High-throughput glycomic analyses reveal unique oligosaccharide profiles of canine and feline milk samples
title_short High-throughput glycomic analyses reveal unique oligosaccharide profiles of canine and feline milk samples
title_sort high-throughput glycomic analyses reveal unique oligosaccharide profiles of canine and feline milk samples
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7714234/
https://www.ncbi.nlm.nih.gov/pubmed/33270789
http://dx.doi.org/10.1371/journal.pone.0243323
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