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Oncofertility case report: live birth 10 years after oocyte in vitro maturation and zygote cryopreservation

PURPOSE: This study aims to report a case of urgent fertility preservation in an oncological patient with collection of immature oocytes in the absence of ovarian stimulation that, through in vitro maturation (IVM), followed by ICSI and cryopreservation of zygotes resulted, 10 years later, in the li...

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Autores principales: Rodrigues, P., Marques, M., Pimentel, S., Rato, M., Carvalho, P., Correia, S. C., Mendes, N., Amaral, H., Fernandes, J. P., Carvalho, M. J., Plancha, C. E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer US 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7714812/
https://www.ncbi.nlm.nih.gov/pubmed/33113072
http://dx.doi.org/10.1007/s10815-020-01984-3
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author Rodrigues, P.
Marques, M.
Pimentel, S.
Rato, M.
Carvalho, P.
Correia, S. C.
Mendes, N.
Amaral, H.
Fernandes, J. P.
Carvalho, M. J.
Plancha, C. E.
author_facet Rodrigues, P.
Marques, M.
Pimentel, S.
Rato, M.
Carvalho, P.
Correia, S. C.
Mendes, N.
Amaral, H.
Fernandes, J. P.
Carvalho, M. J.
Plancha, C. E.
author_sort Rodrigues, P.
collection PubMed
description PURPOSE: This study aims to report a case of urgent fertility preservation in an oncological patient with collection of immature oocytes in the absence of ovarian stimulation that, through in vitro maturation (IVM), followed by ICSI and cryopreservation of zygotes resulted, 10 years later, in the live birth of a healthy baby. METHODS: In September 2008, our clinic performed IVM in a 32-year-old woman diagnosed with a ductal invasive carcinoma with positive estradiol receptors, negative progesterone receptors and positive human epidermal growth factor receptor 2. The retrieval of immature oocytes was performed in the absence of ovarian stimulation after a simple mastectomy and prior to any chemotherapy treatment. The compact cumulus-oocyte complexes (COCs) collected were placed in Lag medium for 2 h, followed by incubation in IVM medium, supplemented with heat inactivated patient serum, recombinant FSH, and recombinant LH. After 30 h in culture, cumulus cells were removed, the metaphase II oocytes were microinjected, and the zygotes obtained were cryopreserved. In 2017, the zygotes were thawed and cultured until day 3. One embryo was transferred and the other cryopreserved. RESULTS: Four compact COCs were collected and subjected to IVM. Two oocytes reached metaphase II and were microinjected. Two zygotes were obtained and were cryopreserved at the two pronuclear stage. Approximately 9 years later, the two zygotes were thawed and cultured until day 3. An embryo with 10 cells was transferred and implanted, resulting in the birth of a healthy baby. CONCLUSIONS: In cases where urgency to start adjuvant therapy requires immediate oocyte collection, IVM may be the only option to obtain fully competent mature oocytes allowing for effective preservation of the reproductive potential.
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spelling pubmed-77148122020-12-24 Oncofertility case report: live birth 10 years after oocyte in vitro maturation and zygote cryopreservation Rodrigues, P. Marques, M. Pimentel, S. Rato, M. Carvalho, P. Correia, S. C. Mendes, N. Amaral, H. Fernandes, J. P. Carvalho, M. J. Plancha, C. E. J Assist Reprod Genet Fertility Preservation PURPOSE: This study aims to report a case of urgent fertility preservation in an oncological patient with collection of immature oocytes in the absence of ovarian stimulation that, through in vitro maturation (IVM), followed by ICSI and cryopreservation of zygotes resulted, 10 years later, in the live birth of a healthy baby. METHODS: In September 2008, our clinic performed IVM in a 32-year-old woman diagnosed with a ductal invasive carcinoma with positive estradiol receptors, negative progesterone receptors and positive human epidermal growth factor receptor 2. The retrieval of immature oocytes was performed in the absence of ovarian stimulation after a simple mastectomy and prior to any chemotherapy treatment. The compact cumulus-oocyte complexes (COCs) collected were placed in Lag medium for 2 h, followed by incubation in IVM medium, supplemented with heat inactivated patient serum, recombinant FSH, and recombinant LH. After 30 h in culture, cumulus cells were removed, the metaphase II oocytes were microinjected, and the zygotes obtained were cryopreserved. In 2017, the zygotes were thawed and cultured until day 3. One embryo was transferred and the other cryopreserved. RESULTS: Four compact COCs were collected and subjected to IVM. Two oocytes reached metaphase II and were microinjected. Two zygotes were obtained and were cryopreserved at the two pronuclear stage. Approximately 9 years later, the two zygotes were thawed and cultured until day 3. An embryo with 10 cells was transferred and implanted, resulting in the birth of a healthy baby. CONCLUSIONS: In cases where urgency to start adjuvant therapy requires immediate oocyte collection, IVM may be the only option to obtain fully competent mature oocytes allowing for effective preservation of the reproductive potential. Springer US 2020-10-28 2020-12 /pmc/articles/PMC7714812/ /pubmed/33113072 http://dx.doi.org/10.1007/s10815-020-01984-3 Text en © The Author(s) 2020, corrected publication 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Fertility Preservation
Rodrigues, P.
Marques, M.
Pimentel, S.
Rato, M.
Carvalho, P.
Correia, S. C.
Mendes, N.
Amaral, H.
Fernandes, J. P.
Carvalho, M. J.
Plancha, C. E.
Oncofertility case report: live birth 10 years after oocyte in vitro maturation and zygote cryopreservation
title Oncofertility case report: live birth 10 years after oocyte in vitro maturation and zygote cryopreservation
title_full Oncofertility case report: live birth 10 years after oocyte in vitro maturation and zygote cryopreservation
title_fullStr Oncofertility case report: live birth 10 years after oocyte in vitro maturation and zygote cryopreservation
title_full_unstemmed Oncofertility case report: live birth 10 years after oocyte in vitro maturation and zygote cryopreservation
title_short Oncofertility case report: live birth 10 years after oocyte in vitro maturation and zygote cryopreservation
title_sort oncofertility case report: live birth 10 years after oocyte in vitro maturation and zygote cryopreservation
topic Fertility Preservation
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7714812/
https://www.ncbi.nlm.nih.gov/pubmed/33113072
http://dx.doi.org/10.1007/s10815-020-01984-3
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