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Expression Profiles of Circular RNA in Human Atrial Fibrillation With Valvular Heart Diseases

Circular RNAs (circRNA) are involved in a variety of human heart diseases, however, circRNA expression profiles and circRNA-miRNA-mRNA regulatory network in human atrial fibrillation (AF) especially with valvular heart diseases (VHD) remain poorly understood. A high-throughput RNA sequencing was use...

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Autores principales: Zhu, Xiyu, Tang, Xinlong, Chong, Hoshun, Cao, Hailong, Fan, Fudong, Pan, Jun, Wang, Dongjin, Zhou, Qing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7714832/
https://www.ncbi.nlm.nih.gov/pubmed/33330659
http://dx.doi.org/10.3389/fcvm.2020.597932
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author Zhu, Xiyu
Tang, Xinlong
Chong, Hoshun
Cao, Hailong
Fan, Fudong
Pan, Jun
Wang, Dongjin
Zhou, Qing
author_facet Zhu, Xiyu
Tang, Xinlong
Chong, Hoshun
Cao, Hailong
Fan, Fudong
Pan, Jun
Wang, Dongjin
Zhou, Qing
author_sort Zhu, Xiyu
collection PubMed
description Circular RNAs (circRNA) are involved in a variety of human heart diseases, however, circRNA expression profiles and circRNA-miRNA-mRNA regulatory network in human atrial fibrillation (AF) especially with valvular heart diseases (VHD) remain poorly understood. A high-throughput RNA sequencing was used to investigate the differentially expressed circRNAs in left atrial appendage from VHD patients with or without persistent AF. The Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis were performed to predict the potential functions of the host genes of differentially expressed circRNA and their downstream targets. CircRNA–miRNA-mRNA regulatory network was constructed to identify mechanisms underlying circRNAs. qRT-PCR and sanger sequencing were further performed to validate the results. Compared with sinus rhythm (SR) patients, there were 3094 upregulated and 4472 downregulated circRNAs in AF patients respectively. The expression of 10 most differentially expressed circRNAs (circ 255-ITGA7, circ 418-KCNN2, circ 13913-MIB1, circ 44670-BARD1, circ 44782-LAMA2, circ 81906-RYR2, circ 35880-ANO5, circ 22249-TNNI3K, circ 3136-TNNI3K, circ 56186-TNNI3K) between SR and persistent AF patients were verified by qRT-PCR. In addition, specific back-splicing sites of these circRNAs was confirmed by sanger sequencing. GO and KEGG pathway analysis indicated that cAMP signal pathway and Wnt signal pathway might play important role in the development of AF in VHD patients, which might be affected by circRNAs. This study provided a preliminary landscape of circRNAs expression profiles which are involved in persistent AF due to VHD, and established the possibility for future related researches in this field.
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spelling pubmed-77148322020-12-15 Expression Profiles of Circular RNA in Human Atrial Fibrillation With Valvular Heart Diseases Zhu, Xiyu Tang, Xinlong Chong, Hoshun Cao, Hailong Fan, Fudong Pan, Jun Wang, Dongjin Zhou, Qing Front Cardiovasc Med Cardiovascular Medicine Circular RNAs (circRNA) are involved in a variety of human heart diseases, however, circRNA expression profiles and circRNA-miRNA-mRNA regulatory network in human atrial fibrillation (AF) especially with valvular heart diseases (VHD) remain poorly understood. A high-throughput RNA sequencing was used to investigate the differentially expressed circRNAs in left atrial appendage from VHD patients with or without persistent AF. The Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis were performed to predict the potential functions of the host genes of differentially expressed circRNA and their downstream targets. CircRNA–miRNA-mRNA regulatory network was constructed to identify mechanisms underlying circRNAs. qRT-PCR and sanger sequencing were further performed to validate the results. Compared with sinus rhythm (SR) patients, there were 3094 upregulated and 4472 downregulated circRNAs in AF patients respectively. The expression of 10 most differentially expressed circRNAs (circ 255-ITGA7, circ 418-KCNN2, circ 13913-MIB1, circ 44670-BARD1, circ 44782-LAMA2, circ 81906-RYR2, circ 35880-ANO5, circ 22249-TNNI3K, circ 3136-TNNI3K, circ 56186-TNNI3K) between SR and persistent AF patients were verified by qRT-PCR. In addition, specific back-splicing sites of these circRNAs was confirmed by sanger sequencing. GO and KEGG pathway analysis indicated that cAMP signal pathway and Wnt signal pathway might play important role in the development of AF in VHD patients, which might be affected by circRNAs. This study provided a preliminary landscape of circRNAs expression profiles which are involved in persistent AF due to VHD, and established the possibility for future related researches in this field. Frontiers Media S.A. 2020-11-20 /pmc/articles/PMC7714832/ /pubmed/33330659 http://dx.doi.org/10.3389/fcvm.2020.597932 Text en Copyright © 2020 Zhu, Tang, Chong, Cao, Fan, Pan, Wang and Zhou. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cardiovascular Medicine
Zhu, Xiyu
Tang, Xinlong
Chong, Hoshun
Cao, Hailong
Fan, Fudong
Pan, Jun
Wang, Dongjin
Zhou, Qing
Expression Profiles of Circular RNA in Human Atrial Fibrillation With Valvular Heart Diseases
title Expression Profiles of Circular RNA in Human Atrial Fibrillation With Valvular Heart Diseases
title_full Expression Profiles of Circular RNA in Human Atrial Fibrillation With Valvular Heart Diseases
title_fullStr Expression Profiles of Circular RNA in Human Atrial Fibrillation With Valvular Heart Diseases
title_full_unstemmed Expression Profiles of Circular RNA in Human Atrial Fibrillation With Valvular Heart Diseases
title_short Expression Profiles of Circular RNA in Human Atrial Fibrillation With Valvular Heart Diseases
title_sort expression profiles of circular rna in human atrial fibrillation with valvular heart diseases
topic Cardiovascular Medicine
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7714832/
https://www.ncbi.nlm.nih.gov/pubmed/33330659
http://dx.doi.org/10.3389/fcvm.2020.597932
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