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Influence of blood contamination on the bond strength and biointeractivity of Biodentine used as root-end filling

AIM: To evaluate the influence of blood contamination on the bond strength and apatite forming ability of Biodentine used as root-end filling material. METHODOLOGY: Eighty (n = 80) extracted single-rooted, sound human maxillary anterior teeth were prepared and obturated. Then, the roots were resecte...

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Autores principales: Shalabi, M., Saber, S., Elsewify, T.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7715484/
https://www.ncbi.nlm.nih.gov/pubmed/33304080
http://dx.doi.org/10.1016/j.sdentj.2019.11.005
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author Shalabi, M.
Saber, S.
Elsewify, T.
author_facet Shalabi, M.
Saber, S.
Elsewify, T.
author_sort Shalabi, M.
collection PubMed
description AIM: To evaluate the influence of blood contamination on the bond strength and apatite forming ability of Biodentine used as root-end filling material. METHODOLOGY: Eighty (n = 80) extracted single-rooted, sound human maxillary anterior teeth were prepared and obturated. Then, the roots were resected, retrograde cavities were prepared and Biodentine was inserted as the root-end filling material. Teeth were then randomly divided into 2 equal groups (n = 40) according to the setting environment of Biodentine i.e., group A where setting took place in human blood and group B where setting took place in deionized water (control group). Teeth were incubated at 37  °C for 45 min to ensure complete setting. Root discs with the filling material in their core were prepared. Push-out bond strength test was performed using a universal testing machine and failure mode was examined. Both groups were aged in HBSS for 30 days. Apatite nucleation was evaluated at one-day, 7-days, and 30-days interval using SEM for morphological analysis and EDX for elemental analysis. Calculation of the Ca/P ratios was performed in addition to XRD for crystal phase analysis. RESULTS: Blood contamination (group A) resulted in significant reduction of bond strength values. It also affected the amount of apatite deposition on the material surface and interfacial spaces with higher Ca/P ratios than that of the normal stoichiometric hydroxyapatite. CONCLUSIONS: Blood contamination during setting of Biodentine had a detrimental effect on the bond strength and reduced the nucleation of apatite in comparison to non-contaminated group.
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spelling pubmed-77154842020-12-09 Influence of blood contamination on the bond strength and biointeractivity of Biodentine used as root-end filling Shalabi, M. Saber, S. Elsewify, T. Saudi Dent J Original Article AIM: To evaluate the influence of blood contamination on the bond strength and apatite forming ability of Biodentine used as root-end filling material. METHODOLOGY: Eighty (n = 80) extracted single-rooted, sound human maxillary anterior teeth were prepared and obturated. Then, the roots were resected, retrograde cavities were prepared and Biodentine was inserted as the root-end filling material. Teeth were then randomly divided into 2 equal groups (n = 40) according to the setting environment of Biodentine i.e., group A where setting took place in human blood and group B where setting took place in deionized water (control group). Teeth were incubated at 37  °C for 45 min to ensure complete setting. Root discs with the filling material in their core were prepared. Push-out bond strength test was performed using a universal testing machine and failure mode was examined. Both groups were aged in HBSS for 30 days. Apatite nucleation was evaluated at one-day, 7-days, and 30-days interval using SEM for morphological analysis and EDX for elemental analysis. Calculation of the Ca/P ratios was performed in addition to XRD for crystal phase analysis. RESULTS: Blood contamination (group A) resulted in significant reduction of bond strength values. It also affected the amount of apatite deposition on the material surface and interfacial spaces with higher Ca/P ratios than that of the normal stoichiometric hydroxyapatite. CONCLUSIONS: Blood contamination during setting of Biodentine had a detrimental effect on the bond strength and reduced the nucleation of apatite in comparison to non-contaminated group. Elsevier 2020-12 2019-11-21 /pmc/articles/PMC7715484/ /pubmed/33304080 http://dx.doi.org/10.1016/j.sdentj.2019.11.005 Text en © 2019 King Saud University http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Original Article
Shalabi, M.
Saber, S.
Elsewify, T.
Influence of blood contamination on the bond strength and biointeractivity of Biodentine used as root-end filling
title Influence of blood contamination on the bond strength and biointeractivity of Biodentine used as root-end filling
title_full Influence of blood contamination on the bond strength and biointeractivity of Biodentine used as root-end filling
title_fullStr Influence of blood contamination on the bond strength and biointeractivity of Biodentine used as root-end filling
title_full_unstemmed Influence of blood contamination on the bond strength and biointeractivity of Biodentine used as root-end filling
title_short Influence of blood contamination on the bond strength and biointeractivity of Biodentine used as root-end filling
title_sort influence of blood contamination on the bond strength and biointeractivity of biodentine used as root-end filling
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7715484/
https://www.ncbi.nlm.nih.gov/pubmed/33304080
http://dx.doi.org/10.1016/j.sdentj.2019.11.005
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