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Influence of blood contamination on the bond strength and biointeractivity of Biodentine used as root-end filling
AIM: To evaluate the influence of blood contamination on the bond strength and apatite forming ability of Biodentine used as root-end filling material. METHODOLOGY: Eighty (n = 80) extracted single-rooted, sound human maxillary anterior teeth were prepared and obturated. Then, the roots were resecte...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7715484/ https://www.ncbi.nlm.nih.gov/pubmed/33304080 http://dx.doi.org/10.1016/j.sdentj.2019.11.005 |
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author | Shalabi, M. Saber, S. Elsewify, T. |
author_facet | Shalabi, M. Saber, S. Elsewify, T. |
author_sort | Shalabi, M. |
collection | PubMed |
description | AIM: To evaluate the influence of blood contamination on the bond strength and apatite forming ability of Biodentine used as root-end filling material. METHODOLOGY: Eighty (n = 80) extracted single-rooted, sound human maxillary anterior teeth were prepared and obturated. Then, the roots were resected, retrograde cavities were prepared and Biodentine was inserted as the root-end filling material. Teeth were then randomly divided into 2 equal groups (n = 40) according to the setting environment of Biodentine i.e., group A where setting took place in human blood and group B where setting took place in deionized water (control group). Teeth were incubated at 37 °C for 45 min to ensure complete setting. Root discs with the filling material in their core were prepared. Push-out bond strength test was performed using a universal testing machine and failure mode was examined. Both groups were aged in HBSS for 30 days. Apatite nucleation was evaluated at one-day, 7-days, and 30-days interval using SEM for morphological analysis and EDX for elemental analysis. Calculation of the Ca/P ratios was performed in addition to XRD for crystal phase analysis. RESULTS: Blood contamination (group A) resulted in significant reduction of bond strength values. It also affected the amount of apatite deposition on the material surface and interfacial spaces with higher Ca/P ratios than that of the normal stoichiometric hydroxyapatite. CONCLUSIONS: Blood contamination during setting of Biodentine had a detrimental effect on the bond strength and reduced the nucleation of apatite in comparison to non-contaminated group. |
format | Online Article Text |
id | pubmed-7715484 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-77154842020-12-09 Influence of blood contamination on the bond strength and biointeractivity of Biodentine used as root-end filling Shalabi, M. Saber, S. Elsewify, T. Saudi Dent J Original Article AIM: To evaluate the influence of blood contamination on the bond strength and apatite forming ability of Biodentine used as root-end filling material. METHODOLOGY: Eighty (n = 80) extracted single-rooted, sound human maxillary anterior teeth were prepared and obturated. Then, the roots were resected, retrograde cavities were prepared and Biodentine was inserted as the root-end filling material. Teeth were then randomly divided into 2 equal groups (n = 40) according to the setting environment of Biodentine i.e., group A where setting took place in human blood and group B where setting took place in deionized water (control group). Teeth were incubated at 37 °C for 45 min to ensure complete setting. Root discs with the filling material in their core were prepared. Push-out bond strength test was performed using a universal testing machine and failure mode was examined. Both groups were aged in HBSS for 30 days. Apatite nucleation was evaluated at one-day, 7-days, and 30-days interval using SEM for morphological analysis and EDX for elemental analysis. Calculation of the Ca/P ratios was performed in addition to XRD for crystal phase analysis. RESULTS: Blood contamination (group A) resulted in significant reduction of bond strength values. It also affected the amount of apatite deposition on the material surface and interfacial spaces with higher Ca/P ratios than that of the normal stoichiometric hydroxyapatite. CONCLUSIONS: Blood contamination during setting of Biodentine had a detrimental effect on the bond strength and reduced the nucleation of apatite in comparison to non-contaminated group. Elsevier 2020-12 2019-11-21 /pmc/articles/PMC7715484/ /pubmed/33304080 http://dx.doi.org/10.1016/j.sdentj.2019.11.005 Text en © 2019 King Saud University http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Original Article Shalabi, M. Saber, S. Elsewify, T. Influence of blood contamination on the bond strength and biointeractivity of Biodentine used as root-end filling |
title | Influence of blood contamination on the bond strength and biointeractivity of Biodentine used as root-end filling |
title_full | Influence of blood contamination on the bond strength and biointeractivity of Biodentine used as root-end filling |
title_fullStr | Influence of blood contamination on the bond strength and biointeractivity of Biodentine used as root-end filling |
title_full_unstemmed | Influence of blood contamination on the bond strength and biointeractivity of Biodentine used as root-end filling |
title_short | Influence of blood contamination on the bond strength and biointeractivity of Biodentine used as root-end filling |
title_sort | influence of blood contamination on the bond strength and biointeractivity of biodentine used as root-end filling |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7715484/ https://www.ncbi.nlm.nih.gov/pubmed/33304080 http://dx.doi.org/10.1016/j.sdentj.2019.11.005 |
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