Cargando…

The Role of Salmonella Genomic Island 4 in Metal Tolerance of Salmonella enterica Serovar I 4,[5],12:i:- Pork Outbreak Isolate USDA15WA-1

Multidrug-resistant (MDR; resistance to >3 antimicrobial classes) Salmonella enterica serovar I 4,[5],12:i:- strains were linked to a 2015 foodborne outbreak from pork. Strain USDA15WA-1, associated with the outbreak, harbors an MDR module and the metal tolerance element Salmonella Genomic Island...

Descripción completa

Detalles Bibliográficos
Autores principales: Bearson, Bradley L., Trachsel, Julian M., Shippy, Daniel C., Sivasankaran, Sathesh K., Kerr, Brian J., Loving, Crystal L., Brunelle, Brian W., Curry, Shelby M., Gabler, Nicholas K., Bearson, Shawn M. D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7716197/
https://www.ncbi.nlm.nih.gov/pubmed/33142960
http://dx.doi.org/10.3390/genes11111291
_version_ 1783619111521091584
author Bearson, Bradley L.
Trachsel, Julian M.
Shippy, Daniel C.
Sivasankaran, Sathesh K.
Kerr, Brian J.
Loving, Crystal L.
Brunelle, Brian W.
Curry, Shelby M.
Gabler, Nicholas K.
Bearson, Shawn M. D.
author_facet Bearson, Bradley L.
Trachsel, Julian M.
Shippy, Daniel C.
Sivasankaran, Sathesh K.
Kerr, Brian J.
Loving, Crystal L.
Brunelle, Brian W.
Curry, Shelby M.
Gabler, Nicholas K.
Bearson, Shawn M. D.
author_sort Bearson, Bradley L.
collection PubMed
description Multidrug-resistant (MDR; resistance to >3 antimicrobial classes) Salmonella enterica serovar I 4,[5],12:i:- strains were linked to a 2015 foodborne outbreak from pork. Strain USDA15WA-1, associated with the outbreak, harbors an MDR module and the metal tolerance element Salmonella Genomic Island 4 (SGI-4). Characterization of SGI-4 revealed that conjugational transfer of SGI-4 resulted in the mobile genetic element (MGE) replicating as a plasmid or integrating into the chromosome. Tolerance to copper, arsenic, and antimony compounds was increased in Salmonella strains containing SGI-4 compared to strains lacking the MGE. Following Salmonella exposure to copper, RNA-seq transcriptional analysis demonstrated significant differential expression of diverse genes and pathways, including induction of at least 38 metal tolerance genes (copper, arsenic, silver, and mercury). Evaluation of swine administered elevated concentrations of zinc oxide (2000 mg/kg) and copper sulfate (200 mg/kg) as an antimicrobial feed additive (Zn+Cu) in their diet for four weeks prior to and three weeks post-inoculation with serovar I 4,[5],12:i:- indicated that Salmonella shedding levels declined at a slower rate in pigs receiving in-feed Zn+Cu compared to control pigs (no Zn+Cu). The presence of metal tolerance genes in MDR Salmonella serovar I 4,[5],12:i:- may provide benefits for environmental survival or swine colonization in metal-containing settings.
format Online
Article
Text
id pubmed-7716197
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-77161972020-12-05 The Role of Salmonella Genomic Island 4 in Metal Tolerance of Salmonella enterica Serovar I 4,[5],12:i:- Pork Outbreak Isolate USDA15WA-1 Bearson, Bradley L. Trachsel, Julian M. Shippy, Daniel C. Sivasankaran, Sathesh K. Kerr, Brian J. Loving, Crystal L. Brunelle, Brian W. Curry, Shelby M. Gabler, Nicholas K. Bearson, Shawn M. D. Genes (Basel) Article Multidrug-resistant (MDR; resistance to >3 antimicrobial classes) Salmonella enterica serovar I 4,[5],12:i:- strains were linked to a 2015 foodborne outbreak from pork. Strain USDA15WA-1, associated with the outbreak, harbors an MDR module and the metal tolerance element Salmonella Genomic Island 4 (SGI-4). Characterization of SGI-4 revealed that conjugational transfer of SGI-4 resulted in the mobile genetic element (MGE) replicating as a plasmid or integrating into the chromosome. Tolerance to copper, arsenic, and antimony compounds was increased in Salmonella strains containing SGI-4 compared to strains lacking the MGE. Following Salmonella exposure to copper, RNA-seq transcriptional analysis demonstrated significant differential expression of diverse genes and pathways, including induction of at least 38 metal tolerance genes (copper, arsenic, silver, and mercury). Evaluation of swine administered elevated concentrations of zinc oxide (2000 mg/kg) and copper sulfate (200 mg/kg) as an antimicrobial feed additive (Zn+Cu) in their diet for four weeks prior to and three weeks post-inoculation with serovar I 4,[5],12:i:- indicated that Salmonella shedding levels declined at a slower rate in pigs receiving in-feed Zn+Cu compared to control pigs (no Zn+Cu). The presence of metal tolerance genes in MDR Salmonella serovar I 4,[5],12:i:- may provide benefits for environmental survival or swine colonization in metal-containing settings. MDPI 2020-10-30 /pmc/articles/PMC7716197/ /pubmed/33142960 http://dx.doi.org/10.3390/genes11111291 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Bearson, Bradley L.
Trachsel, Julian M.
Shippy, Daniel C.
Sivasankaran, Sathesh K.
Kerr, Brian J.
Loving, Crystal L.
Brunelle, Brian W.
Curry, Shelby M.
Gabler, Nicholas K.
Bearson, Shawn M. D.
The Role of Salmonella Genomic Island 4 in Metal Tolerance of Salmonella enterica Serovar I 4,[5],12:i:- Pork Outbreak Isolate USDA15WA-1
title The Role of Salmonella Genomic Island 4 in Metal Tolerance of Salmonella enterica Serovar I 4,[5],12:i:- Pork Outbreak Isolate USDA15WA-1
title_full The Role of Salmonella Genomic Island 4 in Metal Tolerance of Salmonella enterica Serovar I 4,[5],12:i:- Pork Outbreak Isolate USDA15WA-1
title_fullStr The Role of Salmonella Genomic Island 4 in Metal Tolerance of Salmonella enterica Serovar I 4,[5],12:i:- Pork Outbreak Isolate USDA15WA-1
title_full_unstemmed The Role of Salmonella Genomic Island 4 in Metal Tolerance of Salmonella enterica Serovar I 4,[5],12:i:- Pork Outbreak Isolate USDA15WA-1
title_short The Role of Salmonella Genomic Island 4 in Metal Tolerance of Salmonella enterica Serovar I 4,[5],12:i:- Pork Outbreak Isolate USDA15WA-1
title_sort role of salmonella genomic island 4 in metal tolerance of salmonella enterica serovar i 4,[5],12:i:- pork outbreak isolate usda15wa-1
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7716197/
https://www.ncbi.nlm.nih.gov/pubmed/33142960
http://dx.doi.org/10.3390/genes11111291
work_keys_str_mv AT bearsonbradleyl theroleofsalmonellagenomicisland4inmetaltoleranceofsalmonellaentericaserovari4512iporkoutbreakisolateusda15wa1
AT trachseljulianm theroleofsalmonellagenomicisland4inmetaltoleranceofsalmonellaentericaserovari4512iporkoutbreakisolateusda15wa1
AT shippydanielc theroleofsalmonellagenomicisland4inmetaltoleranceofsalmonellaentericaserovari4512iporkoutbreakisolateusda15wa1
AT sivasankaransatheshk theroleofsalmonellagenomicisland4inmetaltoleranceofsalmonellaentericaserovari4512iporkoutbreakisolateusda15wa1
AT kerrbrianj theroleofsalmonellagenomicisland4inmetaltoleranceofsalmonellaentericaserovari4512iporkoutbreakisolateusda15wa1
AT lovingcrystall theroleofsalmonellagenomicisland4inmetaltoleranceofsalmonellaentericaserovari4512iporkoutbreakisolateusda15wa1
AT brunellebrianw theroleofsalmonellagenomicisland4inmetaltoleranceofsalmonellaentericaserovari4512iporkoutbreakisolateusda15wa1
AT curryshelbym theroleofsalmonellagenomicisland4inmetaltoleranceofsalmonellaentericaserovari4512iporkoutbreakisolateusda15wa1
AT gablernicholask theroleofsalmonellagenomicisland4inmetaltoleranceofsalmonellaentericaserovari4512iporkoutbreakisolateusda15wa1
AT bearsonshawnmd theroleofsalmonellagenomicisland4inmetaltoleranceofsalmonellaentericaserovari4512iporkoutbreakisolateusda15wa1
AT bearsonbradleyl roleofsalmonellagenomicisland4inmetaltoleranceofsalmonellaentericaserovari4512iporkoutbreakisolateusda15wa1
AT trachseljulianm roleofsalmonellagenomicisland4inmetaltoleranceofsalmonellaentericaserovari4512iporkoutbreakisolateusda15wa1
AT shippydanielc roleofsalmonellagenomicisland4inmetaltoleranceofsalmonellaentericaserovari4512iporkoutbreakisolateusda15wa1
AT sivasankaransatheshk roleofsalmonellagenomicisland4inmetaltoleranceofsalmonellaentericaserovari4512iporkoutbreakisolateusda15wa1
AT kerrbrianj roleofsalmonellagenomicisland4inmetaltoleranceofsalmonellaentericaserovari4512iporkoutbreakisolateusda15wa1
AT lovingcrystall roleofsalmonellagenomicisland4inmetaltoleranceofsalmonellaentericaserovari4512iporkoutbreakisolateusda15wa1
AT brunellebrianw roleofsalmonellagenomicisland4inmetaltoleranceofsalmonellaentericaserovari4512iporkoutbreakisolateusda15wa1
AT curryshelbym roleofsalmonellagenomicisland4inmetaltoleranceofsalmonellaentericaserovari4512iporkoutbreakisolateusda15wa1
AT gablernicholask roleofsalmonellagenomicisland4inmetaltoleranceofsalmonellaentericaserovari4512iporkoutbreakisolateusda15wa1
AT bearsonshawnmd roleofsalmonellagenomicisland4inmetaltoleranceofsalmonellaentericaserovari4512iporkoutbreakisolateusda15wa1