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Seasonal Patterns of Dominant Microbes Involved in Central Nutrient Cycles in the Subsurface

Microbial communities play a key role for central biogeochemical cycles in the subsurface. Little is known about whether short-term seasonal drought and rewetting events influence the dominant microbes involved in C- and N-cycles. Here, we applied metaproteomics at different subsurface sites in wint...

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Autores principales: Lohmann, Patrick, Benk, Simon, Gleixner, Gerd, Potthast, Karin, Michalzik, Beate, Jehmlich, Nico, von Bergen, Martin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7716230/
https://www.ncbi.nlm.nih.gov/pubmed/33143231
http://dx.doi.org/10.3390/microorganisms8111694
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author Lohmann, Patrick
Benk, Simon
Gleixner, Gerd
Potthast, Karin
Michalzik, Beate
Jehmlich, Nico
von Bergen, Martin
author_facet Lohmann, Patrick
Benk, Simon
Gleixner, Gerd
Potthast, Karin
Michalzik, Beate
Jehmlich, Nico
von Bergen, Martin
author_sort Lohmann, Patrick
collection PubMed
description Microbial communities play a key role for central biogeochemical cycles in the subsurface. Little is known about whether short-term seasonal drought and rewetting events influence the dominant microbes involved in C- and N-cycles. Here, we applied metaproteomics at different subsurface sites in winter, summer and autumn from surface litter layer, seepage water at increasing subsoil depths and remote located groundwater from two wells within the Hainich Critical Zone Exploratory, Germany. We observed changes in the dominance of microbial families at subsurface sampling sites with increasing distances, i.e., Microcoleaceae dominated in topsoil seepage, while Candidatus Brocadiaceae dominated at deeper and more distant groundwater wells. Nitrifying bacteria showed a shift in dominance from drought to rewetting events from summer by Nitrosomandaceae to autumn by Candidatus Brocadiaceae. We further observed that the reductive pentose phosphate pathway was a prominent CO(2)-fixation strategy, dominated by Woeseiaceae in wet early winter, which decreased under drought conditions and changed to a dominance of Sphingobacteriaceae under rewetting conditions. This study shows that increasing subsurface sites and rewetting event after drought alter the dominances of key subsurface microbes. This helps to predict the consequences of annual seasonal dynamics on the nutrient cycling microbes that contribute to ecosystem functioning.
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spelling pubmed-77162302020-12-05 Seasonal Patterns of Dominant Microbes Involved in Central Nutrient Cycles in the Subsurface Lohmann, Patrick Benk, Simon Gleixner, Gerd Potthast, Karin Michalzik, Beate Jehmlich, Nico von Bergen, Martin Microorganisms Article Microbial communities play a key role for central biogeochemical cycles in the subsurface. Little is known about whether short-term seasonal drought and rewetting events influence the dominant microbes involved in C- and N-cycles. Here, we applied metaproteomics at different subsurface sites in winter, summer and autumn from surface litter layer, seepage water at increasing subsoil depths and remote located groundwater from two wells within the Hainich Critical Zone Exploratory, Germany. We observed changes in the dominance of microbial families at subsurface sampling sites with increasing distances, i.e., Microcoleaceae dominated in topsoil seepage, while Candidatus Brocadiaceae dominated at deeper and more distant groundwater wells. Nitrifying bacteria showed a shift in dominance from drought to rewetting events from summer by Nitrosomandaceae to autumn by Candidatus Brocadiaceae. We further observed that the reductive pentose phosphate pathway was a prominent CO(2)-fixation strategy, dominated by Woeseiaceae in wet early winter, which decreased under drought conditions and changed to a dominance of Sphingobacteriaceae under rewetting conditions. This study shows that increasing subsurface sites and rewetting event after drought alter the dominances of key subsurface microbes. This helps to predict the consequences of annual seasonal dynamics on the nutrient cycling microbes that contribute to ecosystem functioning. MDPI 2020-10-30 /pmc/articles/PMC7716230/ /pubmed/33143231 http://dx.doi.org/10.3390/microorganisms8111694 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Lohmann, Patrick
Benk, Simon
Gleixner, Gerd
Potthast, Karin
Michalzik, Beate
Jehmlich, Nico
von Bergen, Martin
Seasonal Patterns of Dominant Microbes Involved in Central Nutrient Cycles in the Subsurface
title Seasonal Patterns of Dominant Microbes Involved in Central Nutrient Cycles in the Subsurface
title_full Seasonal Patterns of Dominant Microbes Involved in Central Nutrient Cycles in the Subsurface
title_fullStr Seasonal Patterns of Dominant Microbes Involved in Central Nutrient Cycles in the Subsurface
title_full_unstemmed Seasonal Patterns of Dominant Microbes Involved in Central Nutrient Cycles in the Subsurface
title_short Seasonal Patterns of Dominant Microbes Involved in Central Nutrient Cycles in the Subsurface
title_sort seasonal patterns of dominant microbes involved in central nutrient cycles in the subsurface
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7716230/
https://www.ncbi.nlm.nih.gov/pubmed/33143231
http://dx.doi.org/10.3390/microorganisms8111694
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