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Computer-controlled liquid-nitrogen drizzling device for removing frost from cryopreserved crystals

Cryocrystallography is a technique that is used more often than room-temperature data collection in macromolecular crystallography. One of its advantages is the significant reduction in radiation damage, which is especially useful in synchrotron experiments. Another advantage is that cryopreservatio...

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Autores principales: Nakamura, Yuki, Baba, Seiki, Mizuno, Nobuhiro, Irie, Takaki, Ueno, Go, Hirata, Kunio, Ito, Sho, Hasegawa, Kazuya, Yamamoto, Masaki, Kumasaka, Takashi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: International Union of Crystallography 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7716257/
https://www.ncbi.nlm.nih.gov/pubmed/33263574
http://dx.doi.org/10.1107/S2053230X2001420X
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author Nakamura, Yuki
Baba, Seiki
Mizuno, Nobuhiro
Irie, Takaki
Ueno, Go
Hirata, Kunio
Ito, Sho
Hasegawa, Kazuya
Yamamoto, Masaki
Kumasaka, Takashi
author_facet Nakamura, Yuki
Baba, Seiki
Mizuno, Nobuhiro
Irie, Takaki
Ueno, Go
Hirata, Kunio
Ito, Sho
Hasegawa, Kazuya
Yamamoto, Masaki
Kumasaka, Takashi
author_sort Nakamura, Yuki
collection PubMed
description Cryocrystallography is a technique that is used more often than room-temperature data collection in macromolecular crystallography. One of its advantages is the significant reduction in radiation damage, which is especially useful in synchrotron experiments. Another advantage is that cryopreservation provides simple storage of crystals and easy transportation to a synchrotron. However, this technique sometimes results in the undesirable adhesion of frost to mounted crystals. The frost produces noisy diffraction images and reduces the optical visibility of crystals, which is crucial for aligning the crystal position with the incident X-ray position. To resolve these issues, a computer-controlled device has been developed that drizzles liquid nitrogen over a crystal to remove frost. It was confirmed that the device works properly, reduces noise from ice rings in diffraction images and enables the centering of crystals with low visibility owing to frost adhesion.
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spelling pubmed-77162572020-12-16 Computer-controlled liquid-nitrogen drizzling device for removing frost from cryopreserved crystals Nakamura, Yuki Baba, Seiki Mizuno, Nobuhiro Irie, Takaki Ueno, Go Hirata, Kunio Ito, Sho Hasegawa, Kazuya Yamamoto, Masaki Kumasaka, Takashi Acta Crystallogr F Struct Biol Commun Isdsb Cryocrystallography is a technique that is used more often than room-temperature data collection in macromolecular crystallography. One of its advantages is the significant reduction in radiation damage, which is especially useful in synchrotron experiments. Another advantage is that cryopreservation provides simple storage of crystals and easy transportation to a synchrotron. However, this technique sometimes results in the undesirable adhesion of frost to mounted crystals. The frost produces noisy diffraction images and reduces the optical visibility of crystals, which is crucial for aligning the crystal position with the incident X-ray position. To resolve these issues, a computer-controlled device has been developed that drizzles liquid nitrogen over a crystal to remove frost. It was confirmed that the device works properly, reduces noise from ice rings in diffraction images and enables the centering of crystals with low visibility owing to frost adhesion. International Union of Crystallography 2020-11-25 /pmc/articles/PMC7716257/ /pubmed/33263574 http://dx.doi.org/10.1107/S2053230X2001420X Text en © Nakamura et al. 2020 http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution (CC-BY) Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited.http://creativecommons.org/licenses/by/4.0/
spellingShingle Isdsb
Nakamura, Yuki
Baba, Seiki
Mizuno, Nobuhiro
Irie, Takaki
Ueno, Go
Hirata, Kunio
Ito, Sho
Hasegawa, Kazuya
Yamamoto, Masaki
Kumasaka, Takashi
Computer-controlled liquid-nitrogen drizzling device for removing frost from cryopreserved crystals
title Computer-controlled liquid-nitrogen drizzling device for removing frost from cryopreserved crystals
title_full Computer-controlled liquid-nitrogen drizzling device for removing frost from cryopreserved crystals
title_fullStr Computer-controlled liquid-nitrogen drizzling device for removing frost from cryopreserved crystals
title_full_unstemmed Computer-controlled liquid-nitrogen drizzling device for removing frost from cryopreserved crystals
title_short Computer-controlled liquid-nitrogen drizzling device for removing frost from cryopreserved crystals
title_sort computer-controlled liquid-nitrogen drizzling device for removing frost from cryopreserved crystals
topic Isdsb
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7716257/
https://www.ncbi.nlm.nih.gov/pubmed/33263574
http://dx.doi.org/10.1107/S2053230X2001420X
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