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Computer-controlled liquid-nitrogen drizzling device for removing frost from cryopreserved crystals
Cryocrystallography is a technique that is used more often than room-temperature data collection in macromolecular crystallography. One of its advantages is the significant reduction in radiation damage, which is especially useful in synchrotron experiments. Another advantage is that cryopreservatio...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
International Union of Crystallography
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7716257/ https://www.ncbi.nlm.nih.gov/pubmed/33263574 http://dx.doi.org/10.1107/S2053230X2001420X |
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author | Nakamura, Yuki Baba, Seiki Mizuno, Nobuhiro Irie, Takaki Ueno, Go Hirata, Kunio Ito, Sho Hasegawa, Kazuya Yamamoto, Masaki Kumasaka, Takashi |
author_facet | Nakamura, Yuki Baba, Seiki Mizuno, Nobuhiro Irie, Takaki Ueno, Go Hirata, Kunio Ito, Sho Hasegawa, Kazuya Yamamoto, Masaki Kumasaka, Takashi |
author_sort | Nakamura, Yuki |
collection | PubMed |
description | Cryocrystallography is a technique that is used more often than room-temperature data collection in macromolecular crystallography. One of its advantages is the significant reduction in radiation damage, which is especially useful in synchrotron experiments. Another advantage is that cryopreservation provides simple storage of crystals and easy transportation to a synchrotron. However, this technique sometimes results in the undesirable adhesion of frost to mounted crystals. The frost produces noisy diffraction images and reduces the optical visibility of crystals, which is crucial for aligning the crystal position with the incident X-ray position. To resolve these issues, a computer-controlled device has been developed that drizzles liquid nitrogen over a crystal to remove frost. It was confirmed that the device works properly, reduces noise from ice rings in diffraction images and enables the centering of crystals with low visibility owing to frost adhesion. |
format | Online Article Text |
id | pubmed-7716257 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | International Union of Crystallography |
record_format | MEDLINE/PubMed |
spelling | pubmed-77162572020-12-16 Computer-controlled liquid-nitrogen drizzling device for removing frost from cryopreserved crystals Nakamura, Yuki Baba, Seiki Mizuno, Nobuhiro Irie, Takaki Ueno, Go Hirata, Kunio Ito, Sho Hasegawa, Kazuya Yamamoto, Masaki Kumasaka, Takashi Acta Crystallogr F Struct Biol Commun Isdsb Cryocrystallography is a technique that is used more often than room-temperature data collection in macromolecular crystallography. One of its advantages is the significant reduction in radiation damage, which is especially useful in synchrotron experiments. Another advantage is that cryopreservation provides simple storage of crystals and easy transportation to a synchrotron. However, this technique sometimes results in the undesirable adhesion of frost to mounted crystals. The frost produces noisy diffraction images and reduces the optical visibility of crystals, which is crucial for aligning the crystal position with the incident X-ray position. To resolve these issues, a computer-controlled device has been developed that drizzles liquid nitrogen over a crystal to remove frost. It was confirmed that the device works properly, reduces noise from ice rings in diffraction images and enables the centering of crystals with low visibility owing to frost adhesion. International Union of Crystallography 2020-11-25 /pmc/articles/PMC7716257/ /pubmed/33263574 http://dx.doi.org/10.1107/S2053230X2001420X Text en © Nakamura et al. 2020 http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution (CC-BY) Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited.http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Isdsb Nakamura, Yuki Baba, Seiki Mizuno, Nobuhiro Irie, Takaki Ueno, Go Hirata, Kunio Ito, Sho Hasegawa, Kazuya Yamamoto, Masaki Kumasaka, Takashi Computer-controlled liquid-nitrogen drizzling device for removing frost from cryopreserved crystals |
title | Computer-controlled liquid-nitrogen drizzling device for removing frost from cryopreserved crystals |
title_full | Computer-controlled liquid-nitrogen drizzling device for removing frost from cryopreserved crystals |
title_fullStr | Computer-controlled liquid-nitrogen drizzling device for removing frost from cryopreserved crystals |
title_full_unstemmed | Computer-controlled liquid-nitrogen drizzling device for removing frost from cryopreserved crystals |
title_short | Computer-controlled liquid-nitrogen drizzling device for removing frost from cryopreserved crystals |
title_sort | computer-controlled liquid-nitrogen drizzling device for removing frost from cryopreserved crystals |
topic | Isdsb |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7716257/ https://www.ncbi.nlm.nih.gov/pubmed/33263574 http://dx.doi.org/10.1107/S2053230X2001420X |
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