The A component (SmhA) of a tripartite pore-forming toxin from Serratia marcescens: expression, purification and crystallographic analysis

Tripartite α-pore-forming toxins are constructed of three proteins (A, B and C) and are found in many bacterial pathogens. While structures of the B and C components from Gram-negative bacteria have been described, the structure of the A component of a Gram-negative α-pore-forming toxin has so far p...

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Autores principales: Churchill-Angus, Alicia M., Sedelnikova, Svetlana E., Schofield, Thomas H. B., Baker, Patrick J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: International Union of Crystallography 2020
Materias:
Research Communications
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7716259/
https://www.ncbi.nlm.nih.gov/pubmed/33263568
http://dx.doi.org/10.1107/S2053230X20013862
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author Churchill-Angus, Alicia M.
Sedelnikova, Svetlana E.
Schofield, Thomas H. B.
Baker, Patrick J.
author_facet Churchill-Angus, Alicia M.
Sedelnikova, Svetlana E.
Schofield, Thomas H. B.
Baker, Patrick J.
author_sort Churchill-Angus, Alicia M.
collection PubMed
description Tripartite α-pore-forming toxins are constructed of three proteins (A, B and C) and are found in many bacterial pathogens. While structures of the B and C components from Gram-negative bacteria have been described, the structure of the A component of a Gram-negative α-pore-forming toxin has so far proved elusive. SmhA, the A component from the opportunistic human pathogen Serratia marcescens, has been cloned, overexpressed and purified. Crystals were grown of selenomethionine-derivatized protein and anomalous data were collected. Phases were calculated and an initial electron-density map was produced.
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spelling pubmed-77162592020-12-16 The A component (SmhA) of a tripartite pore-forming toxin from Serratia marcescens: expression, purification and crystallographic analysis Churchill-Angus, Alicia M. Sedelnikova, Svetlana E. Schofield, Thomas H. B. Baker, Patrick J. Acta Crystallogr F Struct Biol Commun Research Communications Tripartite α-pore-forming toxins are constructed of three proteins (A, B and C) and are found in many bacterial pathogens. While structures of the B and C components from Gram-negative bacteria have been described, the structure of the A component of a Gram-negative α-pore-forming toxin has so far proved elusive. SmhA, the A component from the opportunistic human pathogen Serratia marcescens, has been cloned, overexpressed and purified. Crystals were grown of selenomethionine-derivatized protein and anomalous data were collected. Phases were calculated and an initial electron-density map was produced. International Union of Crystallography 2020-11-25 /pmc/articles/PMC7716259/ /pubmed/33263568 http://dx.doi.org/10.1107/S2053230X20013862 Text en © Churchill-Angus et al. 2020 http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution (CC-BY) Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited.http://creativecommons.org/licenses/by/4.0/
spellingShingle Research Communications
Churchill-Angus, Alicia M.
Sedelnikova, Svetlana E.
Schofield, Thomas H. B.
Baker, Patrick J.
The A component (SmhA) of a tripartite pore-forming toxin from Serratia marcescens: expression, purification and crystallographic analysis
title The A component (SmhA) of a tripartite pore-forming toxin from Serratia marcescens: expression, purification and crystallographic analysis
title_full The A component (SmhA) of a tripartite pore-forming toxin from Serratia marcescens: expression, purification and crystallographic analysis
title_fullStr The A component (SmhA) of a tripartite pore-forming toxin from Serratia marcescens: expression, purification and crystallographic analysis
title_full_unstemmed The A component (SmhA) of a tripartite pore-forming toxin from Serratia marcescens: expression, purification and crystallographic analysis
title_short The A component (SmhA) of a tripartite pore-forming toxin from Serratia marcescens: expression, purification and crystallographic analysis
title_sort a component (smha) of a tripartite pore-forming toxin from serratia marcescens: expression, purification and crystallographic analysis
topic Research Communications
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7716259/
https://www.ncbi.nlm.nih.gov/pubmed/33263568
http://dx.doi.org/10.1107/S2053230X20013862
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