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RNA sequencing: new technologies and applications in cancer research
Over the past few decades, RNA sequencing has significantly progressed, becoming a paramount approach for transcriptome profiling. The revolution from bulk RNA sequencing to single-molecular, single-cell and spatial transcriptome approaches has enabled increasingly accurate, individual cell resoluti...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7716291/ https://www.ncbi.nlm.nih.gov/pubmed/33276803 http://dx.doi.org/10.1186/s13045-020-01005-x |
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author | Hong, Mingye Tao, Shuang Zhang, Ling Diao, Li-Ting Huang, Xuanmei Huang, Shaohui Xie, Shu-Juan Xiao, Zhen-Dong Zhang, Hua |
author_facet | Hong, Mingye Tao, Shuang Zhang, Ling Diao, Li-Ting Huang, Xuanmei Huang, Shaohui Xie, Shu-Juan Xiao, Zhen-Dong Zhang, Hua |
author_sort | Hong, Mingye |
collection | PubMed |
description | Over the past few decades, RNA sequencing has significantly progressed, becoming a paramount approach for transcriptome profiling. The revolution from bulk RNA sequencing to single-molecular, single-cell and spatial transcriptome approaches has enabled increasingly accurate, individual cell resolution incorporated with spatial information. Cancer, a major malignant and heterogeneous lethal disease, remains an enormous challenge in medical research and clinical treatment. As a vital tool, RNA sequencing has been utilized in many aspects of cancer research and therapy, including biomarker discovery and characterization of cancer heterogeneity and evolution, drug resistance, cancer immune microenvironment and immunotherapy, cancer neoantigens and so on. In this review, the latest studies on RNA sequencing technology and their applications in cancer are summarized, and future challenges and opportunities for RNA sequencing technology in cancer applications are discussed. |
format | Online Article Text |
id | pubmed-7716291 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-77162912020-12-04 RNA sequencing: new technologies and applications in cancer research Hong, Mingye Tao, Shuang Zhang, Ling Diao, Li-Ting Huang, Xuanmei Huang, Shaohui Xie, Shu-Juan Xiao, Zhen-Dong Zhang, Hua J Hematol Oncol Review Over the past few decades, RNA sequencing has significantly progressed, becoming a paramount approach for transcriptome profiling. The revolution from bulk RNA sequencing to single-molecular, single-cell and spatial transcriptome approaches has enabled increasingly accurate, individual cell resolution incorporated with spatial information. Cancer, a major malignant and heterogeneous lethal disease, remains an enormous challenge in medical research and clinical treatment. As a vital tool, RNA sequencing has been utilized in many aspects of cancer research and therapy, including biomarker discovery and characterization of cancer heterogeneity and evolution, drug resistance, cancer immune microenvironment and immunotherapy, cancer neoantigens and so on. In this review, the latest studies on RNA sequencing technology and their applications in cancer are summarized, and future challenges and opportunities for RNA sequencing technology in cancer applications are discussed. BioMed Central 2020-12-04 /pmc/articles/PMC7716291/ /pubmed/33276803 http://dx.doi.org/10.1186/s13045-020-01005-x Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Review Hong, Mingye Tao, Shuang Zhang, Ling Diao, Li-Ting Huang, Xuanmei Huang, Shaohui Xie, Shu-Juan Xiao, Zhen-Dong Zhang, Hua RNA sequencing: new technologies and applications in cancer research |
title | RNA sequencing: new technologies and applications in cancer research |
title_full | RNA sequencing: new technologies and applications in cancer research |
title_fullStr | RNA sequencing: new technologies and applications in cancer research |
title_full_unstemmed | RNA sequencing: new technologies and applications in cancer research |
title_short | RNA sequencing: new technologies and applications in cancer research |
title_sort | rna sequencing: new technologies and applications in cancer research |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7716291/ https://www.ncbi.nlm.nih.gov/pubmed/33276803 http://dx.doi.org/10.1186/s13045-020-01005-x |
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