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PlantLayout pipeline to model tissue patterning

To study the mechanisms underlying developmental pattern formation in a tissue, one needs to analyze the dynamics of the regulators in time and space across the tissue of a specific architecture. This problem is essential for the developmental regulators (morphogens) that distribute over the tissues...

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Autores principales: Savina, M.S., Mironova, V.V.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Federal Research Center Institute of Cytology and Genetics of Siberian Branch of the Russian Academy of Sciences 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7716512/
https://www.ncbi.nlm.nih.gov/pubmed/33659787
http://dx.doi.org/10.18699/VJ20.590
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author Savina, M.S.
Mironova, V.V.
author_facet Savina, M.S.
Mironova, V.V.
author_sort Savina, M.S.
collection PubMed
description To study the mechanisms underlying developmental pattern formation in a tissue, one needs to analyze the dynamics of the regulators in time and space across the tissue of a specific architecture. This problem is essential for the developmental regulators (morphogens) that distribute over the tissues anisotropically, forming there maxima and gradients and guiding cellular processes in a dose-dependent manner. Here we present the PlantLayout pipeline for MATLAB software, which facilitates the computational studies of tissue patterning. With its help, one can build a structural model of a two-dimensional tissue, embed it into a mathematical model in ODEs, perform numerical simulations, and visualize the obtained results – everything on the same platform. As a result, one can study the concentration dynamics of developmental regulators over the cell layout reconstructed from the real tissue. PlantLayout allows studying the dynamics and the output of gene networks guided by the developmental regulator in specific cells. The gene networks could be different for different cell types. One of the obstacles that PlantLayout removes semi-automatically is the determination of the cell wall orientation which is relevant when cells in the tissue have a polarity. Additionally, PlantLayout allows automatically extracting other quantitative and qualitative features of the cells and the cell walls, which might help in the modeling of a developmental pattern, such as the length and the width of the cell walls, the set of the neighboring cells, cell volume and cell perimeter. We demonstrate PlantLayout performance on the model of phytohormone auxin distribution over the plant root tip.
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spelling pubmed-77165122021-03-02 PlantLayout pipeline to model tissue patterning Savina, M.S. Mironova, V.V. Vavilovskii Zhurnal Genet Selektsii Original Article To study the mechanisms underlying developmental pattern formation in a tissue, one needs to analyze the dynamics of the regulators in time and space across the tissue of a specific architecture. This problem is essential for the developmental regulators (morphogens) that distribute over the tissues anisotropically, forming there maxima and gradients and guiding cellular processes in a dose-dependent manner. Here we present the PlantLayout pipeline for MATLAB software, which facilitates the computational studies of tissue patterning. With its help, one can build a structural model of a two-dimensional tissue, embed it into a mathematical model in ODEs, perform numerical simulations, and visualize the obtained results – everything on the same platform. As a result, one can study the concentration dynamics of developmental regulators over the cell layout reconstructed from the real tissue. PlantLayout allows studying the dynamics and the output of gene networks guided by the developmental regulator in specific cells. The gene networks could be different for different cell types. One of the obstacles that PlantLayout removes semi-automatically is the determination of the cell wall orientation which is relevant when cells in the tissue have a polarity. Additionally, PlantLayout allows automatically extracting other quantitative and qualitative features of the cells and the cell walls, which might help in the modeling of a developmental pattern, such as the length and the width of the cell walls, the set of the neighboring cells, cell volume and cell perimeter. We demonstrate PlantLayout performance on the model of phytohormone auxin distribution over the plant root tip. The Federal Research Center Institute of Cytology and Genetics of Siberian Branch of the Russian Academy of Sciences 2020-02 /pmc/articles/PMC7716512/ /pubmed/33659787 http://dx.doi.org/10.18699/VJ20.590 Text en Copyright © AUTHORS, 2018 http://creativecommons.org/licenses/by/2.5/ This work is licensed under a Creative Commons Attribution 4.0 License
spellingShingle Original Article
Savina, M.S.
Mironova, V.V.
PlantLayout pipeline to model tissue patterning
title PlantLayout pipeline to model tissue patterning
title_full PlantLayout pipeline to model tissue patterning
title_fullStr PlantLayout pipeline to model tissue patterning
title_full_unstemmed PlantLayout pipeline to model tissue patterning
title_short PlantLayout pipeline to model tissue patterning
title_sort plantlayout pipeline to model tissue patterning
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7716512/
https://www.ncbi.nlm.nih.gov/pubmed/33659787
http://dx.doi.org/10.18699/VJ20.590
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