An effective combination of codon optimization, gene dosage, and process optimization for high-level production of fibrinolytic enzyme in Komagataella phaffii (Pichia pastoris)

BACKGROUND: As a main drug for diseased thrombus, some clinically used thrombolytic agents have various disadvantages, safer novel thrombolytic agents are of great demand. This study aimed to achieve high and efficient production of a fibrinolytic enzyme with superior enzymatic properties, by a comb...

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Detalles Bibliográficos
Autores principales: Che, Zhiqun, Cao, Xiaoyan, Chen, Guiguang, Liang, Zhiqun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2020
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7716587/
https://www.ncbi.nlm.nih.gov/pubmed/33276774
http://dx.doi.org/10.1186/s12896-020-00654-7

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7716587/
https://www.ncbi.nlm.nih.gov/pubmed/33276774
http://dx.doi.org/10.1186/s12896-020-00654-7

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