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High Representation of Archaea Across All Depths in Oxic and Low-pH Sediment Layers Underlying an Acidic Stream
Parys Mountain or Mynydd Parys (Isle of Anglesey, United Kingdom) is a mine-impacted environment, which accommodates a variety of acidophilic organisms. Our previous research of water and sediments from one of the surface acidic streams showed a high proportion of archaea in the total microbial comm...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7716880/ https://www.ncbi.nlm.nih.gov/pubmed/33329440 http://dx.doi.org/10.3389/fmicb.2020.576520 |
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author | Distaso, Marco A. Bargiela, Rafael Brailsford, Francesca L. Williams, Gwion B. Wright, Samuel Lunev, Evgenii A. Toshchakov, Stepan V. Yakimov, Michail M. Jones, David L. Golyshin, Peter N. Golyshina, Olga V. |
author_facet | Distaso, Marco A. Bargiela, Rafael Brailsford, Francesca L. Williams, Gwion B. Wright, Samuel Lunev, Evgenii A. Toshchakov, Stepan V. Yakimov, Michail M. Jones, David L. Golyshin, Peter N. Golyshina, Olga V. |
author_sort | Distaso, Marco A. |
collection | PubMed |
description | Parys Mountain or Mynydd Parys (Isle of Anglesey, United Kingdom) is a mine-impacted environment, which accommodates a variety of acidophilic organisms. Our previous research of water and sediments from one of the surface acidic streams showed a high proportion of archaea in the total microbial community. To understand the spatial distribution of archaea, we sampled cores (0–20 cm) of sediment and conducted chemical analyses and taxonomic profiling of microbiomes using 16S rRNA gene amplicon sequencing in different core layers. The taxonomic affiliation of sequencing reads indicated that archaea represented between 6.2 and 54% of the microbial community at all sediment depths. Majority of archaea were associated with the order Thermoplasmatales, with the most abundant group of sequences being clustered closely with the phylotype B_DKE, followed by “E-plasma,” “A-plasma,” other yet uncultured Thermoplasmatales with Ferroplasma and Cuniculiplasma spp. represented in minor proportions. Thermoplasmatales were found at all depths and in the whole range of chemical conditions with their abundance correlating with sediment Fe, As, Cr, and Mn contents. The bacterial microbiome component was largely composed in all layers of sediment by members of the phyla Proteobacteria, Actinobacteria, Nitrospirae, Firmicutes, uncultured Chloroflexi (AD3 group), and Acidobacteria. This study has revealed a high abundance of Thermoplasmatales in acid mine drainage-affected sediment layers and pointed at these organisms being the main contributors to carbon, and probably to iron and sulfur cycles in this ecosystem. |
format | Online Article Text |
id | pubmed-7716880 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-77168802020-12-15 High Representation of Archaea Across All Depths in Oxic and Low-pH Sediment Layers Underlying an Acidic Stream Distaso, Marco A. Bargiela, Rafael Brailsford, Francesca L. Williams, Gwion B. Wright, Samuel Lunev, Evgenii A. Toshchakov, Stepan V. Yakimov, Michail M. Jones, David L. Golyshin, Peter N. Golyshina, Olga V. Front Microbiol Microbiology Parys Mountain or Mynydd Parys (Isle of Anglesey, United Kingdom) is a mine-impacted environment, which accommodates a variety of acidophilic organisms. Our previous research of water and sediments from one of the surface acidic streams showed a high proportion of archaea in the total microbial community. To understand the spatial distribution of archaea, we sampled cores (0–20 cm) of sediment and conducted chemical analyses and taxonomic profiling of microbiomes using 16S rRNA gene amplicon sequencing in different core layers. The taxonomic affiliation of sequencing reads indicated that archaea represented between 6.2 and 54% of the microbial community at all sediment depths. Majority of archaea were associated with the order Thermoplasmatales, with the most abundant group of sequences being clustered closely with the phylotype B_DKE, followed by “E-plasma,” “A-plasma,” other yet uncultured Thermoplasmatales with Ferroplasma and Cuniculiplasma spp. represented in minor proportions. Thermoplasmatales were found at all depths and in the whole range of chemical conditions with their abundance correlating with sediment Fe, As, Cr, and Mn contents. The bacterial microbiome component was largely composed in all layers of sediment by members of the phyla Proteobacteria, Actinobacteria, Nitrospirae, Firmicutes, uncultured Chloroflexi (AD3 group), and Acidobacteria. This study has revealed a high abundance of Thermoplasmatales in acid mine drainage-affected sediment layers and pointed at these organisms being the main contributors to carbon, and probably to iron and sulfur cycles in this ecosystem. Frontiers Media S.A. 2020-11-17 /pmc/articles/PMC7716880/ /pubmed/33329440 http://dx.doi.org/10.3389/fmicb.2020.576520 Text en Copyright © 2020 Distaso, Bargiela, Brailsford, Williams, Wright, Lunev, Toshchakov, Yakimov, Jones, Golyshin and Golyshina. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Distaso, Marco A. Bargiela, Rafael Brailsford, Francesca L. Williams, Gwion B. Wright, Samuel Lunev, Evgenii A. Toshchakov, Stepan V. Yakimov, Michail M. Jones, David L. Golyshin, Peter N. Golyshina, Olga V. High Representation of Archaea Across All Depths in Oxic and Low-pH Sediment Layers Underlying an Acidic Stream |
title | High Representation of Archaea Across All Depths in Oxic and Low-pH Sediment Layers Underlying an Acidic Stream |
title_full | High Representation of Archaea Across All Depths in Oxic and Low-pH Sediment Layers Underlying an Acidic Stream |
title_fullStr | High Representation of Archaea Across All Depths in Oxic and Low-pH Sediment Layers Underlying an Acidic Stream |
title_full_unstemmed | High Representation of Archaea Across All Depths in Oxic and Low-pH Sediment Layers Underlying an Acidic Stream |
title_short | High Representation of Archaea Across All Depths in Oxic and Low-pH Sediment Layers Underlying an Acidic Stream |
title_sort | high representation of archaea across all depths in oxic and low-ph sediment layers underlying an acidic stream |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7716880/ https://www.ncbi.nlm.nih.gov/pubmed/33329440 http://dx.doi.org/10.3389/fmicb.2020.576520 |
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