Cargando…

First isolation, in-vivo and genomic characterization of zoonotic variegated squirrel Bornavirus 1 (VSBV-1) isolates

The variegated squirrel bornavirus 1 (VSBV-1), a member of the family Bornaviridae, was discovered in 2015 in a series of lethal human infections. Screening approaches revealed kept exotic squirrels as the putative source of infection. Infectious virus was successfully isolated by co-cultivation of...

Descripción completa

Detalles Bibliográficos
Autores principales: Schlottau, Kore, Nobach, Daniel, Herden, Christiane, Finke, Stefan, Beer, Martin, Hoffmann, Donata
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7717607/
https://www.ncbi.nlm.nih.gov/pubmed/33151793
http://dx.doi.org/10.1080/22221751.2020.1847604
_version_ 1783619340667453440
author Schlottau, Kore
Nobach, Daniel
Herden, Christiane
Finke, Stefan
Beer, Martin
Hoffmann, Donata
author_facet Schlottau, Kore
Nobach, Daniel
Herden, Christiane
Finke, Stefan
Beer, Martin
Hoffmann, Donata
author_sort Schlottau, Kore
collection PubMed
description The variegated squirrel bornavirus 1 (VSBV-1), a member of the family Bornaviridae, was discovered in 2015 in a series of lethal human infections. Screening approaches revealed kept exotic squirrels as the putative source of infection. Infectious virus was successfully isolated by co-cultivation of infected primary squirrel cells with permanent cell lines. For in vivo characterization, neonatal and adult Lewis rats were inoculated either intracranially, intranasally or subcutaneously. After 4.5 months, three out of fifteen neonatal intracranially inoculated rats were VSBV-1 genome positive in the central nervous system without showing clinical signs. Pathohistological examination revealed a non-purulent encephalitis. While infection of immune incompetent rats (neonatal) using the type species of mammalian bornaviruses, the Borna disease virus 1, proceed to an immune tolerant status, VSBV-1 infection could result in inflammation of neuronal tissue. Sequencing showed minor adaptations within the VSBV-1 genome comparing to the viral genomes from infected squirrels, cell cultures or rat tissues. In conclusion, we were able to generate the first VSBV-1 isolates and provide in vivo animal model data in Lewis rats revealing substantial differences between VSBV-1 and BoDV-1. Furthermore, the presented data are a precondition for insights into the transmission and pathogenesis of this novel zoonotic pathogen.
format Online
Article
Text
id pubmed-7717607
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher Taylor & Francis
record_format MEDLINE/PubMed
spelling pubmed-77176072020-12-10 First isolation, in-vivo and genomic characterization of zoonotic variegated squirrel Bornavirus 1 (VSBV-1) isolates Schlottau, Kore Nobach, Daniel Herden, Christiane Finke, Stefan Beer, Martin Hoffmann, Donata Emerg Microbes Infect Research Article The variegated squirrel bornavirus 1 (VSBV-1), a member of the family Bornaviridae, was discovered in 2015 in a series of lethal human infections. Screening approaches revealed kept exotic squirrels as the putative source of infection. Infectious virus was successfully isolated by co-cultivation of infected primary squirrel cells with permanent cell lines. For in vivo characterization, neonatal and adult Lewis rats were inoculated either intracranially, intranasally or subcutaneously. After 4.5 months, three out of fifteen neonatal intracranially inoculated rats were VSBV-1 genome positive in the central nervous system without showing clinical signs. Pathohistological examination revealed a non-purulent encephalitis. While infection of immune incompetent rats (neonatal) using the type species of mammalian bornaviruses, the Borna disease virus 1, proceed to an immune tolerant status, VSBV-1 infection could result in inflammation of neuronal tissue. Sequencing showed minor adaptations within the VSBV-1 genome comparing to the viral genomes from infected squirrels, cell cultures or rat tissues. In conclusion, we were able to generate the first VSBV-1 isolates and provide in vivo animal model data in Lewis rats revealing substantial differences between VSBV-1 and BoDV-1. Furthermore, the presented data are a precondition for insights into the transmission and pathogenesis of this novel zoonotic pathogen. Taylor & Francis 2020-11-20 /pmc/articles/PMC7717607/ /pubmed/33151793 http://dx.doi.org/10.1080/22221751.2020.1847604 Text en © 2020 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group, on behalf of Shanghai Shangyixun Cultural Communication Co., Ltd https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Schlottau, Kore
Nobach, Daniel
Herden, Christiane
Finke, Stefan
Beer, Martin
Hoffmann, Donata
First isolation, in-vivo and genomic characterization of zoonotic variegated squirrel Bornavirus 1 (VSBV-1) isolates
title First isolation, in-vivo and genomic characterization of zoonotic variegated squirrel Bornavirus 1 (VSBV-1) isolates
title_full First isolation, in-vivo and genomic characterization of zoonotic variegated squirrel Bornavirus 1 (VSBV-1) isolates
title_fullStr First isolation, in-vivo and genomic characterization of zoonotic variegated squirrel Bornavirus 1 (VSBV-1) isolates
title_full_unstemmed First isolation, in-vivo and genomic characterization of zoonotic variegated squirrel Bornavirus 1 (VSBV-1) isolates
title_short First isolation, in-vivo and genomic characterization of zoonotic variegated squirrel Bornavirus 1 (VSBV-1) isolates
title_sort first isolation, in-vivo and genomic characterization of zoonotic variegated squirrel bornavirus 1 (vsbv-1) isolates
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7717607/
https://www.ncbi.nlm.nih.gov/pubmed/33151793
http://dx.doi.org/10.1080/22221751.2020.1847604
work_keys_str_mv AT schlottaukore firstisolationinvivoandgenomiccharacterizationofzoonoticvariegatedsquirrelbornavirus1vsbv1isolates
AT nobachdaniel firstisolationinvivoandgenomiccharacterizationofzoonoticvariegatedsquirrelbornavirus1vsbv1isolates
AT herdenchristiane firstisolationinvivoandgenomiccharacterizationofzoonoticvariegatedsquirrelbornavirus1vsbv1isolates
AT finkestefan firstisolationinvivoandgenomiccharacterizationofzoonoticvariegatedsquirrelbornavirus1vsbv1isolates
AT beermartin firstisolationinvivoandgenomiccharacterizationofzoonoticvariegatedsquirrelbornavirus1vsbv1isolates
AT hoffmanndonata firstisolationinvivoandgenomiccharacterizationofzoonoticvariegatedsquirrelbornavirus1vsbv1isolates