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Nitric Oxide Mediates Inflammation in Type II Diabetes Mellitus through the PPARγ/eNOS Signaling Pathway

Inflammation accounts for the process of type II diabetes mellitus (T2DM), the specific mechanism of which is still to be elucidated yet. Nitric oxide (NO), a critical inflammation regulator, the role of which is the inflammation of T2DM, is rarely reported. Therefore, our study is aimed at explorin...

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Autores principales: Guo, Hua, Zhang, Qinglan, Yuan, Haipo, Zhou, Lin, Li, Fang-fang, Wang, Sheng-Ming, Shi, Gang, Wang, Maojuan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7718064/
https://www.ncbi.nlm.nih.gov/pubmed/33293942
http://dx.doi.org/10.1155/2020/8889612
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author Guo, Hua
Zhang, Qinglan
Yuan, Haipo
Zhou, Lin
Li, Fang-fang
Wang, Sheng-Ming
Shi, Gang
Wang, Maojuan
author_facet Guo, Hua
Zhang, Qinglan
Yuan, Haipo
Zhou, Lin
Li, Fang-fang
Wang, Sheng-Ming
Shi, Gang
Wang, Maojuan
author_sort Guo, Hua
collection PubMed
description Inflammation accounts for the process of type II diabetes mellitus (T2DM), the specific mechanism of which is still to be elucidated yet. Nitric oxide (NO), a critical inflammation regulator, the role of which is the inflammation of T2DM, is rarely reported. Therefore, our study is aimed at exploring the effect of NO on the inflammation in T2DM and the corresponding mechanism. We analyzed the NO levels in plasma samples from T2DM patients and paired healthy adults by Nitric Oxide Analyzer then measured the expression of inflammatory cytokines (C-reactive protein, heptoglobin, IL-1β, TNF-α, IL-6) in insulin-induced HepG2 cells treated with NO donor or NO scavenger, and the PPARγ, eNOS, C-reactive protein, heptoglobin, IL-1β, TNF-α, and IL-6 levels were detected by RT-PCR and western blot in insulin-induced HepG2 cells transfected with si-PPARγ. The results showed that excess NO increased the inflammation marker levels in T2DM, which is activated by the PPARγ/eNOS pathway. These findings will strengthen the understanding of NO in T2DM and provide a new target for the treatment of T2DM.
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spelling pubmed-77180642020-12-07 Nitric Oxide Mediates Inflammation in Type II Diabetes Mellitus through the PPARγ/eNOS Signaling Pathway Guo, Hua Zhang, Qinglan Yuan, Haipo Zhou, Lin Li, Fang-fang Wang, Sheng-Ming Shi, Gang Wang, Maojuan PPAR Res Research Article Inflammation accounts for the process of type II diabetes mellitus (T2DM), the specific mechanism of which is still to be elucidated yet. Nitric oxide (NO), a critical inflammation regulator, the role of which is the inflammation of T2DM, is rarely reported. Therefore, our study is aimed at exploring the effect of NO on the inflammation in T2DM and the corresponding mechanism. We analyzed the NO levels in plasma samples from T2DM patients and paired healthy adults by Nitric Oxide Analyzer then measured the expression of inflammatory cytokines (C-reactive protein, heptoglobin, IL-1β, TNF-α, IL-6) in insulin-induced HepG2 cells treated with NO donor or NO scavenger, and the PPARγ, eNOS, C-reactive protein, heptoglobin, IL-1β, TNF-α, and IL-6 levels were detected by RT-PCR and western blot in insulin-induced HepG2 cells transfected with si-PPARγ. The results showed that excess NO increased the inflammation marker levels in T2DM, which is activated by the PPARγ/eNOS pathway. These findings will strengthen the understanding of NO in T2DM and provide a new target for the treatment of T2DM. Hindawi 2020-11-26 /pmc/articles/PMC7718064/ /pubmed/33293942 http://dx.doi.org/10.1155/2020/8889612 Text en Copyright © 2020 Hua Guo et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Guo, Hua
Zhang, Qinglan
Yuan, Haipo
Zhou, Lin
Li, Fang-fang
Wang, Sheng-Ming
Shi, Gang
Wang, Maojuan
Nitric Oxide Mediates Inflammation in Type II Diabetes Mellitus through the PPARγ/eNOS Signaling Pathway
title Nitric Oxide Mediates Inflammation in Type II Diabetes Mellitus through the PPARγ/eNOS Signaling Pathway
title_full Nitric Oxide Mediates Inflammation in Type II Diabetes Mellitus through the PPARγ/eNOS Signaling Pathway
title_fullStr Nitric Oxide Mediates Inflammation in Type II Diabetes Mellitus through the PPARγ/eNOS Signaling Pathway
title_full_unstemmed Nitric Oxide Mediates Inflammation in Type II Diabetes Mellitus through the PPARγ/eNOS Signaling Pathway
title_short Nitric Oxide Mediates Inflammation in Type II Diabetes Mellitus through the PPARγ/eNOS Signaling Pathway
title_sort nitric oxide mediates inflammation in type ii diabetes mellitus through the pparγ/enos signaling pathway
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7718064/
https://www.ncbi.nlm.nih.gov/pubmed/33293942
http://dx.doi.org/10.1155/2020/8889612
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