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Clinical characteristics influence cultivable-bacteria composition in the meconium of Indonesian neonates

BACKGROUND: Microbial colonization of a neonate's gastrointestinal tract has significant perinatal and lifetime health consequences. However, information regarding the profile of meconium microbiota in neonates and the influence of clinical parameters are lacking in the Indonesian population. T...

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Autores principales: Jonathan, Kristien Juni Thandwi, Ong, Grasella, Prasetyaningsih, Firsty Amanah, Amandito, Radhian, Rohsiswatmo, Rinawati, Malik, Amarila
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7718453/
https://www.ncbi.nlm.nih.gov/pubmed/33305047
http://dx.doi.org/10.1016/j.heliyon.2020.e05576
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author Jonathan, Kristien Juni Thandwi
Ong, Grasella
Prasetyaningsih, Firsty Amanah
Amandito, Radhian
Rohsiswatmo, Rinawati
Malik, Amarila
author_facet Jonathan, Kristien Juni Thandwi
Ong, Grasella
Prasetyaningsih, Firsty Amanah
Amandito, Radhian
Rohsiswatmo, Rinawati
Malik, Amarila
author_sort Jonathan, Kristien Juni Thandwi
collection PubMed
description BACKGROUND: Microbial colonization of a neonate's gastrointestinal tract has significant perinatal and lifetime health consequences. However, information regarding the profile of meconium microbiota in neonates and the influence of clinical parameters are lacking in the Indonesian population. This study aimed to preliminary investigate the profile of cultivable bacterial diversity of meconium isolated from neonates born at Cipto Mangunkusumo Hospital (CMH), Jakarta. The cultivable bacteria were isolated from meconium samples and were then processed for cultivation and molecular identification. RESULTS: Fourteen neonates were enrolled as described, i.e., seven hyperbilirubinemia (Hyp) and seven non-Hyp with ten neonates delivered by cesarean section (CS) and four others by vaginal route (VR), and with five exclusive breastfeeding (Ebf), four formula milk, and five combinations. Microbiological identification, molecular 16S rDNA PCR-Sanger sequencing, and PCA analysis of cultivable bacteria isolated from meconium showed Firmicutes' predominance (84.41%), with an abundant population of Staphylococcus, which consist of S. hominis, S. epidermidis, and S. haemolyticus. The influence of mode of delivery showed a lower diversity than the CS populates the VR, but their composition was similar. Concurrently, between feeding patterns, the genera profile did not show much difference; in the non-Ebf group, the total amount of Staphylococcus and Bacillus showed a higher amount but a less diverse. Interestingly, the non-Hyp group showed more abundant and diverse Staphylococcus than that of the Hyp group. In contrast, neonates diagnosed with NEC and proven sepsis showed the same pattern of Staphylococcus domination. CONCLUSION: Staphylococcus predominated the composition of cultivable bacteria in neonates meconium. Due to the small sample size, only the hyperbilirubinemia parameter significantly influenced the profile, i.e., Staphylococcus's proportion (p = 0.037).
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spelling pubmed-77184532020-12-09 Clinical characteristics influence cultivable-bacteria composition in the meconium of Indonesian neonates Jonathan, Kristien Juni Thandwi Ong, Grasella Prasetyaningsih, Firsty Amanah Amandito, Radhian Rohsiswatmo, Rinawati Malik, Amarila Heliyon Research Article BACKGROUND: Microbial colonization of a neonate's gastrointestinal tract has significant perinatal and lifetime health consequences. However, information regarding the profile of meconium microbiota in neonates and the influence of clinical parameters are lacking in the Indonesian population. This study aimed to preliminary investigate the profile of cultivable bacterial diversity of meconium isolated from neonates born at Cipto Mangunkusumo Hospital (CMH), Jakarta. The cultivable bacteria were isolated from meconium samples and were then processed for cultivation and molecular identification. RESULTS: Fourteen neonates were enrolled as described, i.e., seven hyperbilirubinemia (Hyp) and seven non-Hyp with ten neonates delivered by cesarean section (CS) and four others by vaginal route (VR), and with five exclusive breastfeeding (Ebf), four formula milk, and five combinations. Microbiological identification, molecular 16S rDNA PCR-Sanger sequencing, and PCA analysis of cultivable bacteria isolated from meconium showed Firmicutes' predominance (84.41%), with an abundant population of Staphylococcus, which consist of S. hominis, S. epidermidis, and S. haemolyticus. The influence of mode of delivery showed a lower diversity than the CS populates the VR, but their composition was similar. Concurrently, between feeding patterns, the genera profile did not show much difference; in the non-Ebf group, the total amount of Staphylococcus and Bacillus showed a higher amount but a less diverse. Interestingly, the non-Hyp group showed more abundant and diverse Staphylococcus than that of the Hyp group. In contrast, neonates diagnosed with NEC and proven sepsis showed the same pattern of Staphylococcus domination. CONCLUSION: Staphylococcus predominated the composition of cultivable bacteria in neonates meconium. Due to the small sample size, only the hyperbilirubinemia parameter significantly influenced the profile, i.e., Staphylococcus's proportion (p = 0.037). Elsevier 2020-12-02 /pmc/articles/PMC7718453/ /pubmed/33305047 http://dx.doi.org/10.1016/j.heliyon.2020.e05576 Text en © 2020 The Author(s) http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Research Article
Jonathan, Kristien Juni Thandwi
Ong, Grasella
Prasetyaningsih, Firsty Amanah
Amandito, Radhian
Rohsiswatmo, Rinawati
Malik, Amarila
Clinical characteristics influence cultivable-bacteria composition in the meconium of Indonesian neonates
title Clinical characteristics influence cultivable-bacteria composition in the meconium of Indonesian neonates
title_full Clinical characteristics influence cultivable-bacteria composition in the meconium of Indonesian neonates
title_fullStr Clinical characteristics influence cultivable-bacteria composition in the meconium of Indonesian neonates
title_full_unstemmed Clinical characteristics influence cultivable-bacteria composition in the meconium of Indonesian neonates
title_short Clinical characteristics influence cultivable-bacteria composition in the meconium of Indonesian neonates
title_sort clinical characteristics influence cultivable-bacteria composition in the meconium of indonesian neonates
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7718453/
https://www.ncbi.nlm.nih.gov/pubmed/33305047
http://dx.doi.org/10.1016/j.heliyon.2020.e05576
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