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Nondestructive Phenomic Tools for the Prediction of Heat and Drought Tolerance at Anthesis in Brassica Species

Oilseed Brassica species are vulnerable to heat and drought stress, especially in the early reproductive stage. We evaluated plant imaging of whole plant and flower tissue, leaf stomatal conductance, leaf and bud temperature, photochemical reflectance index, quantum yield of photosynthesis, and leaf...

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Autores principales: Chen, Sheng, Guo, Yiming, Sirault, Xavier, Stefanova, Katia, Saradadevi, Renu, Turner, Neil C., Nelson, Matthew N., Furbank, Robert T., Siddique, Kadambot H. M., Cowling, Wallace A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: AAAS 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7718632/
https://www.ncbi.nlm.nih.gov/pubmed/33313525
http://dx.doi.org/10.34133/2019/3264872
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author Chen, Sheng
Guo, Yiming
Sirault, Xavier
Stefanova, Katia
Saradadevi, Renu
Turner, Neil C.
Nelson, Matthew N.
Furbank, Robert T.
Siddique, Kadambot H. M.
Cowling, Wallace A.
author_facet Chen, Sheng
Guo, Yiming
Sirault, Xavier
Stefanova, Katia
Saradadevi, Renu
Turner, Neil C.
Nelson, Matthew N.
Furbank, Robert T.
Siddique, Kadambot H. M.
Cowling, Wallace A.
author_sort Chen, Sheng
collection PubMed
description Oilseed Brassica species are vulnerable to heat and drought stress, especially in the early reproductive stage. We evaluated plant imaging of whole plant and flower tissue, leaf stomatal conductance, leaf and bud temperature, photochemical reflectance index, quantum yield of photosynthesis, and leaf gas exchange for their suitability to detect tolerance to heat (H) and/or drought (D) stress treatments in 12 Brassica genotypes (G). A replicated factorial experiment was set up with 7 d of stress treatment from the beginning of anthesis with various levels of three factors H, D, and G. Most phenomics tools detected plant stress as indicated by significant main effects of H, D, and H×D. Whole plant volume was highly correlated with fresh weight changes, suggesting that whole plant imaging may be a useful surrogate for fresh weight in future studies. Vcmax, the maximum carboxylation rate of photosynthesis, increased rapidly on day 1 in H and H+D treatments, and there were significant interactions of G×H and G×D. Vcmax of genotypes on day 1 in H and H+D treatments was positively correlated with their harvested seed yield. Vcmax on day 1 and day 3 were clustered with seed yield in H and H+D treatments as shown in the heatmaps of genotypic correlations. TPU, the rate of triose phosphate use, also showed significant positive genotypic correlations with seed yield in H+D treatments. Flower volume showed significant interactions of G×H and G×D on day 7, and flower volume of genotypes on day 7 in H was positively correlated with their harvested seed yield. There were few interactions of G×H or G×D for leaf stomatal conductance, leaf and bud temperature, photochemical reflectance index, and quantum yield of photosynthesis. Vcmax, TPU, and volume of flowers are potential nondestructive phenomic traits for heat or combined heat and drought stress tolerance screening in Brassica germplasm.
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spelling pubmed-77186322020-12-10 Nondestructive Phenomic Tools for the Prediction of Heat and Drought Tolerance at Anthesis in Brassica Species Chen, Sheng Guo, Yiming Sirault, Xavier Stefanova, Katia Saradadevi, Renu Turner, Neil C. Nelson, Matthew N. Furbank, Robert T. Siddique, Kadambot H. M. Cowling, Wallace A. Plant Phenomics Research Article Oilseed Brassica species are vulnerable to heat and drought stress, especially in the early reproductive stage. We evaluated plant imaging of whole plant and flower tissue, leaf stomatal conductance, leaf and bud temperature, photochemical reflectance index, quantum yield of photosynthesis, and leaf gas exchange for their suitability to detect tolerance to heat (H) and/or drought (D) stress treatments in 12 Brassica genotypes (G). A replicated factorial experiment was set up with 7 d of stress treatment from the beginning of anthesis with various levels of three factors H, D, and G. Most phenomics tools detected plant stress as indicated by significant main effects of H, D, and H×D. Whole plant volume was highly correlated with fresh weight changes, suggesting that whole plant imaging may be a useful surrogate for fresh weight in future studies. Vcmax, the maximum carboxylation rate of photosynthesis, increased rapidly on day 1 in H and H+D treatments, and there were significant interactions of G×H and G×D. Vcmax of genotypes on day 1 in H and H+D treatments was positively correlated with their harvested seed yield. Vcmax on day 1 and day 3 were clustered with seed yield in H and H+D treatments as shown in the heatmaps of genotypic correlations. TPU, the rate of triose phosphate use, also showed significant positive genotypic correlations with seed yield in H+D treatments. Flower volume showed significant interactions of G×H and G×D on day 7, and flower volume of genotypes on day 7 in H was positively correlated with their harvested seed yield. There were few interactions of G×H or G×D for leaf stomatal conductance, leaf and bud temperature, photochemical reflectance index, and quantum yield of photosynthesis. Vcmax, TPU, and volume of flowers are potential nondestructive phenomic traits for heat or combined heat and drought stress tolerance screening in Brassica germplasm. AAAS 2019-05-22 /pmc/articles/PMC7718632/ /pubmed/33313525 http://dx.doi.org/10.34133/2019/3264872 Text en Copyright © 2019 Sheng Chen et al. https://creativecommons.org/licenses/by/4.0/ Exclusive licensee Nanjing Agricultural University. Distributed under a Creative Commons Attribution License (CC BY 4.0).
spellingShingle Research Article
Chen, Sheng
Guo, Yiming
Sirault, Xavier
Stefanova, Katia
Saradadevi, Renu
Turner, Neil C.
Nelson, Matthew N.
Furbank, Robert T.
Siddique, Kadambot H. M.
Cowling, Wallace A.
Nondestructive Phenomic Tools for the Prediction of Heat and Drought Tolerance at Anthesis in Brassica Species
title Nondestructive Phenomic Tools for the Prediction of Heat and Drought Tolerance at Anthesis in Brassica Species
title_full Nondestructive Phenomic Tools for the Prediction of Heat and Drought Tolerance at Anthesis in Brassica Species
title_fullStr Nondestructive Phenomic Tools for the Prediction of Heat and Drought Tolerance at Anthesis in Brassica Species
title_full_unstemmed Nondestructive Phenomic Tools for the Prediction of Heat and Drought Tolerance at Anthesis in Brassica Species
title_short Nondestructive Phenomic Tools for the Prediction of Heat and Drought Tolerance at Anthesis in Brassica Species
title_sort nondestructive phenomic tools for the prediction of heat and drought tolerance at anthesis in brassica species
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7718632/
https://www.ncbi.nlm.nih.gov/pubmed/33313525
http://dx.doi.org/10.34133/2019/3264872
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