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Tracking the dynamics of individual gut microbiome of sea cucumber Apostichopus japonicus during gut regeneration

Sea cucumbers possess the remarkable capacity to regenerate their body parts or organs. Regeneration of host organs and/or body parts involves reconstruction of the host associated microbiota, however, the dynamics and contribution of microbiota to the regeneration process are largely unknown due to...

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Autores principales: Yamazaki, Yohei, Sakai, Yuichi, Yu, Juanwen, Mino, Sayaka, Sawabe, Tomoo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: PeerJ Inc. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7718794/
https://www.ncbi.nlm.nih.gov/pubmed/33344070
http://dx.doi.org/10.7717/peerj.10260
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author Yamazaki, Yohei
Sakai, Yuichi
Yu, Juanwen
Mino, Sayaka
Sawabe, Tomoo
author_facet Yamazaki, Yohei
Sakai, Yuichi
Yu, Juanwen
Mino, Sayaka
Sawabe, Tomoo
author_sort Yamazaki, Yohei
collection PubMed
description Sea cucumbers possess the remarkable capacity to regenerate their body parts or organs. Regeneration of host organs and/or body parts involves reconstruction of the host associated microbiota, however, the dynamics and contribution of microbiota to the regeneration process are largely unknown due to a lack of experimental models. To track the dynamics of individual gut microbiomes during gut regeneration, both caged mariculture and laboratory isolator systems of sea cucumbers (Apostichopus japonicus) were developed and longitudinal meta16S analyses were performed. Under natural environmental conditions in the caged mariculture system, both bacterial and eukaryotic communities in sea cucumbers’ guts appeared to be reconstructed within 4 months after evisceration. Using the laboratory isolator, which can trace daily dynamics, we found that fecal microbiota collected before evisceration were clearly different from those collected after evisceration. We also identified eight key bacteria, belonging to Alteromonadaceae, Rhodobacteraceae, Oceanospirillaceae and family-unassigned Gammaproteobacteria, suggesting that these bacteria might interact with the host during the gut regeneration process. Six of the eight key bacteria were isolated for further bioassay using the isolator developed in this study to test whether these isolates affect gut regeneration.
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spelling pubmed-77187942020-12-17 Tracking the dynamics of individual gut microbiome of sea cucumber Apostichopus japonicus during gut regeneration Yamazaki, Yohei Sakai, Yuichi Yu, Juanwen Mino, Sayaka Sawabe, Tomoo PeerJ Aquaculture, Fisheries and Fish Science Sea cucumbers possess the remarkable capacity to regenerate their body parts or organs. Regeneration of host organs and/or body parts involves reconstruction of the host associated microbiota, however, the dynamics and contribution of microbiota to the regeneration process are largely unknown due to a lack of experimental models. To track the dynamics of individual gut microbiomes during gut regeneration, both caged mariculture and laboratory isolator systems of sea cucumbers (Apostichopus japonicus) were developed and longitudinal meta16S analyses were performed. Under natural environmental conditions in the caged mariculture system, both bacterial and eukaryotic communities in sea cucumbers’ guts appeared to be reconstructed within 4 months after evisceration. Using the laboratory isolator, which can trace daily dynamics, we found that fecal microbiota collected before evisceration were clearly different from those collected after evisceration. We also identified eight key bacteria, belonging to Alteromonadaceae, Rhodobacteraceae, Oceanospirillaceae and family-unassigned Gammaproteobacteria, suggesting that these bacteria might interact with the host during the gut regeneration process. Six of the eight key bacteria were isolated for further bioassay using the isolator developed in this study to test whether these isolates affect gut regeneration. PeerJ Inc. 2020-12-01 /pmc/articles/PMC7718794/ /pubmed/33344070 http://dx.doi.org/10.7717/peerj.10260 Text en © 2020 Yamazaki et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited.
spellingShingle Aquaculture, Fisheries and Fish Science
Yamazaki, Yohei
Sakai, Yuichi
Yu, Juanwen
Mino, Sayaka
Sawabe, Tomoo
Tracking the dynamics of individual gut microbiome of sea cucumber Apostichopus japonicus during gut regeneration
title Tracking the dynamics of individual gut microbiome of sea cucumber Apostichopus japonicus during gut regeneration
title_full Tracking the dynamics of individual gut microbiome of sea cucumber Apostichopus japonicus during gut regeneration
title_fullStr Tracking the dynamics of individual gut microbiome of sea cucumber Apostichopus japonicus during gut regeneration
title_full_unstemmed Tracking the dynamics of individual gut microbiome of sea cucumber Apostichopus japonicus during gut regeneration
title_short Tracking the dynamics of individual gut microbiome of sea cucumber Apostichopus japonicus during gut regeneration
title_sort tracking the dynamics of individual gut microbiome of sea cucumber apostichopus japonicus during gut regeneration
topic Aquaculture, Fisheries and Fish Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7718794/
https://www.ncbi.nlm.nih.gov/pubmed/33344070
http://dx.doi.org/10.7717/peerj.10260
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