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Salivary microbial changes during the first 6 months of orthodontic treatment
BACKGROUND: Orthodontic treatment is widely used to treat malocclusion. However, the influence of treatment on the oral microbiome remains unclear. In this study, we investigated salivary microbial changes in patients undergoing orthodontic treatment. METHODS: In total, 19 orthodontic patients parti...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
PeerJ Inc.
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7718796/ https://www.ncbi.nlm.nih.gov/pubmed/33344084 http://dx.doi.org/10.7717/peerj.10446 |
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author | Zhao, Mei Liu, Min Chen, Wei Zhang, Haiping Bai, Yuxing Ren, Wen |
author_facet | Zhao, Mei Liu, Min Chen, Wei Zhang, Haiping Bai, Yuxing Ren, Wen |
author_sort | Zhao, Mei |
collection | PubMed |
description | BACKGROUND: Orthodontic treatment is widely used to treat malocclusion. However, the influence of treatment on the oral microbiome remains unclear. In this study, we investigated salivary microbial changes in patients undergoing orthodontic treatment. METHODS: In total, 19 orthodontic patients participated in this study. Saliva samples were collected at the following three timepoints: before (T0) and 3 months (T1) and 6 months (T2) after the placement of orthodontic appliances. High-throughput sequencing was performed based on the 16S rRNA gene V4 region. RESULTS: The phyla of Proteobacteria, Bacteroidetes, Firmicutes, Actinobacteria and Fusobacteria were predominant. Observed Species, Chao1 and ACE, which represent α diversity, were significantly decreased at T1 and subsequently increased at T2. In addition, the β diversity at T1 based on the Bray-Curtis distances differed from T0 and T2. The relative abundances of Prevotella, Porphyromonas and Peptostreptococcus were decreased with treatment, whereas those of Capnocytophaga and Neisseria exhibited the opposite results. In total, 385 of 410 operational taxonomic units were shared at T0, T1 and T2. The co-occurrence networks with hub nodes at T1 were the most complex. CONCLUSION: Orthodontic treatment temporarily affected the saliva microbial community. This dynamic alteration in species did not induce deterioration in oral health. Oral hygiene instructions were necessary and should be emphasized during each visit. Further studies with longer observation periods and more participants are required. |
format | Online Article Text |
id | pubmed-7718796 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | PeerJ Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-77187962020-12-17 Salivary microbial changes during the first 6 months of orthodontic treatment Zhao, Mei Liu, Min Chen, Wei Zhang, Haiping Bai, Yuxing Ren, Wen PeerJ Microbiology BACKGROUND: Orthodontic treatment is widely used to treat malocclusion. However, the influence of treatment on the oral microbiome remains unclear. In this study, we investigated salivary microbial changes in patients undergoing orthodontic treatment. METHODS: In total, 19 orthodontic patients participated in this study. Saliva samples were collected at the following three timepoints: before (T0) and 3 months (T1) and 6 months (T2) after the placement of orthodontic appliances. High-throughput sequencing was performed based on the 16S rRNA gene V4 region. RESULTS: The phyla of Proteobacteria, Bacteroidetes, Firmicutes, Actinobacteria and Fusobacteria were predominant. Observed Species, Chao1 and ACE, which represent α diversity, were significantly decreased at T1 and subsequently increased at T2. In addition, the β diversity at T1 based on the Bray-Curtis distances differed from T0 and T2. The relative abundances of Prevotella, Porphyromonas and Peptostreptococcus were decreased with treatment, whereas those of Capnocytophaga and Neisseria exhibited the opposite results. In total, 385 of 410 operational taxonomic units were shared at T0, T1 and T2. The co-occurrence networks with hub nodes at T1 were the most complex. CONCLUSION: Orthodontic treatment temporarily affected the saliva microbial community. This dynamic alteration in species did not induce deterioration in oral health. Oral hygiene instructions were necessary and should be emphasized during each visit. Further studies with longer observation periods and more participants are required. PeerJ Inc. 2020-12-01 /pmc/articles/PMC7718796/ /pubmed/33344084 http://dx.doi.org/10.7717/peerj.10446 Text en ©2020 Zhao et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited. |
spellingShingle | Microbiology Zhao, Mei Liu, Min Chen, Wei Zhang, Haiping Bai, Yuxing Ren, Wen Salivary microbial changes during the first 6 months of orthodontic treatment |
title | Salivary microbial changes during the first 6 months of orthodontic treatment |
title_full | Salivary microbial changes during the first 6 months of orthodontic treatment |
title_fullStr | Salivary microbial changes during the first 6 months of orthodontic treatment |
title_full_unstemmed | Salivary microbial changes during the first 6 months of orthodontic treatment |
title_short | Salivary microbial changes during the first 6 months of orthodontic treatment |
title_sort | salivary microbial changes during the first 6 months of orthodontic treatment |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7718796/ https://www.ncbi.nlm.nih.gov/pubmed/33344084 http://dx.doi.org/10.7717/peerj.10446 |
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