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Arabidopsis calcium-dependent protein kinase 3 regulates actin cytoskeleton organization and immunity

Pattern-triggered immunity and effector-triggered immunity are two primary forms of innate immunity in land plants. The molecular components and connecting nodes of pattern-triggered immunity and effector-triggered immunity are not fully understood. Here, we report that the Arabidopsis calcium-depen...

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Autores principales: Lu, Yi-Ju, Li, Pai, Shimono, Masaki, Corrion, Alex, Higaki, Takumi, He, Sheng Yang, Day, Brad
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7718926/
https://www.ncbi.nlm.nih.gov/pubmed/33277490
http://dx.doi.org/10.1038/s41467-020-20007-4
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author Lu, Yi-Ju
Li, Pai
Shimono, Masaki
Corrion, Alex
Higaki, Takumi
He, Sheng Yang
Day, Brad
author_facet Lu, Yi-Ju
Li, Pai
Shimono, Masaki
Corrion, Alex
Higaki, Takumi
He, Sheng Yang
Day, Brad
author_sort Lu, Yi-Ju
collection PubMed
description Pattern-triggered immunity and effector-triggered immunity are two primary forms of innate immunity in land plants. The molecular components and connecting nodes of pattern-triggered immunity and effector-triggered immunity are not fully understood. Here, we report that the Arabidopsis calcium-dependent protein kinase CPK3 is a key regulator of both pattern-triggered immunity and effector-triggered immunity. In vitro and in vivo phosphorylation assays, coupled with genetic and cell biology-based analyses, show that actin-depolymerization factor 4 (ADF4) is a physiological substrate of CPK3, and that phosphorylation of ADF4 by CPK3 governs actin cytoskeletal organization associated with pattern-triggered immunity. CPK3 regulates stomatal closure induced by flg22 and is required for resistance to Pst DC3000. Our data further demonstrates that CPK3 is required for resistance to Pst DC3000 carrying the effector AvrPphB. These results suggest that CPK3 is a missing link between cytoskeleton organization, pattern-triggered immunity and effector-triggered immunity.
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spelling pubmed-77189262020-12-07 Arabidopsis calcium-dependent protein kinase 3 regulates actin cytoskeleton organization and immunity Lu, Yi-Ju Li, Pai Shimono, Masaki Corrion, Alex Higaki, Takumi He, Sheng Yang Day, Brad Nat Commun Article Pattern-triggered immunity and effector-triggered immunity are two primary forms of innate immunity in land plants. The molecular components and connecting nodes of pattern-triggered immunity and effector-triggered immunity are not fully understood. Here, we report that the Arabidopsis calcium-dependent protein kinase CPK3 is a key regulator of both pattern-triggered immunity and effector-triggered immunity. In vitro and in vivo phosphorylation assays, coupled with genetic and cell biology-based analyses, show that actin-depolymerization factor 4 (ADF4) is a physiological substrate of CPK3, and that phosphorylation of ADF4 by CPK3 governs actin cytoskeletal organization associated with pattern-triggered immunity. CPK3 regulates stomatal closure induced by flg22 and is required for resistance to Pst DC3000. Our data further demonstrates that CPK3 is required for resistance to Pst DC3000 carrying the effector AvrPphB. These results suggest that CPK3 is a missing link between cytoskeleton organization, pattern-triggered immunity and effector-triggered immunity. Nature Publishing Group UK 2020-12-04 /pmc/articles/PMC7718926/ /pubmed/33277490 http://dx.doi.org/10.1038/s41467-020-20007-4 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Lu, Yi-Ju
Li, Pai
Shimono, Masaki
Corrion, Alex
Higaki, Takumi
He, Sheng Yang
Day, Brad
Arabidopsis calcium-dependent protein kinase 3 regulates actin cytoskeleton organization and immunity
title Arabidopsis calcium-dependent protein kinase 3 regulates actin cytoskeleton organization and immunity
title_full Arabidopsis calcium-dependent protein kinase 3 regulates actin cytoskeleton organization and immunity
title_fullStr Arabidopsis calcium-dependent protein kinase 3 regulates actin cytoskeleton organization and immunity
title_full_unstemmed Arabidopsis calcium-dependent protein kinase 3 regulates actin cytoskeleton organization and immunity
title_short Arabidopsis calcium-dependent protein kinase 3 regulates actin cytoskeleton organization and immunity
title_sort arabidopsis calcium-dependent protein kinase 3 regulates actin cytoskeleton organization and immunity
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7718926/
https://www.ncbi.nlm.nih.gov/pubmed/33277490
http://dx.doi.org/10.1038/s41467-020-20007-4
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