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Arabidopsis calcium-dependent protein kinase 3 regulates actin cytoskeleton organization and immunity
Pattern-triggered immunity and effector-triggered immunity are two primary forms of innate immunity in land plants. The molecular components and connecting nodes of pattern-triggered immunity and effector-triggered immunity are not fully understood. Here, we report that the Arabidopsis calcium-depen...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7718926/ https://www.ncbi.nlm.nih.gov/pubmed/33277490 http://dx.doi.org/10.1038/s41467-020-20007-4 |
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author | Lu, Yi-Ju Li, Pai Shimono, Masaki Corrion, Alex Higaki, Takumi He, Sheng Yang Day, Brad |
author_facet | Lu, Yi-Ju Li, Pai Shimono, Masaki Corrion, Alex Higaki, Takumi He, Sheng Yang Day, Brad |
author_sort | Lu, Yi-Ju |
collection | PubMed |
description | Pattern-triggered immunity and effector-triggered immunity are two primary forms of innate immunity in land plants. The molecular components and connecting nodes of pattern-triggered immunity and effector-triggered immunity are not fully understood. Here, we report that the Arabidopsis calcium-dependent protein kinase CPK3 is a key regulator of both pattern-triggered immunity and effector-triggered immunity. In vitro and in vivo phosphorylation assays, coupled with genetic and cell biology-based analyses, show that actin-depolymerization factor 4 (ADF4) is a physiological substrate of CPK3, and that phosphorylation of ADF4 by CPK3 governs actin cytoskeletal organization associated with pattern-triggered immunity. CPK3 regulates stomatal closure induced by flg22 and is required for resistance to Pst DC3000. Our data further demonstrates that CPK3 is required for resistance to Pst DC3000 carrying the effector AvrPphB. These results suggest that CPK3 is a missing link between cytoskeleton organization, pattern-triggered immunity and effector-triggered immunity. |
format | Online Article Text |
id | pubmed-7718926 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-77189262020-12-07 Arabidopsis calcium-dependent protein kinase 3 regulates actin cytoskeleton organization and immunity Lu, Yi-Ju Li, Pai Shimono, Masaki Corrion, Alex Higaki, Takumi He, Sheng Yang Day, Brad Nat Commun Article Pattern-triggered immunity and effector-triggered immunity are two primary forms of innate immunity in land plants. The molecular components and connecting nodes of pattern-triggered immunity and effector-triggered immunity are not fully understood. Here, we report that the Arabidopsis calcium-dependent protein kinase CPK3 is a key regulator of both pattern-triggered immunity and effector-triggered immunity. In vitro and in vivo phosphorylation assays, coupled with genetic and cell biology-based analyses, show that actin-depolymerization factor 4 (ADF4) is a physiological substrate of CPK3, and that phosphorylation of ADF4 by CPK3 governs actin cytoskeletal organization associated with pattern-triggered immunity. CPK3 regulates stomatal closure induced by flg22 and is required for resistance to Pst DC3000. Our data further demonstrates that CPK3 is required for resistance to Pst DC3000 carrying the effector AvrPphB. These results suggest that CPK3 is a missing link between cytoskeleton organization, pattern-triggered immunity and effector-triggered immunity. Nature Publishing Group UK 2020-12-04 /pmc/articles/PMC7718926/ /pubmed/33277490 http://dx.doi.org/10.1038/s41467-020-20007-4 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Lu, Yi-Ju Li, Pai Shimono, Masaki Corrion, Alex Higaki, Takumi He, Sheng Yang Day, Brad Arabidopsis calcium-dependent protein kinase 3 regulates actin cytoskeleton organization and immunity |
title | Arabidopsis calcium-dependent protein kinase 3 regulates actin cytoskeleton organization and immunity |
title_full | Arabidopsis calcium-dependent protein kinase 3 regulates actin cytoskeleton organization and immunity |
title_fullStr | Arabidopsis calcium-dependent protein kinase 3 regulates actin cytoskeleton organization and immunity |
title_full_unstemmed | Arabidopsis calcium-dependent protein kinase 3 regulates actin cytoskeleton organization and immunity |
title_short | Arabidopsis calcium-dependent protein kinase 3 regulates actin cytoskeleton organization and immunity |
title_sort | arabidopsis calcium-dependent protein kinase 3 regulates actin cytoskeleton organization and immunity |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7718926/ https://www.ncbi.nlm.nih.gov/pubmed/33277490 http://dx.doi.org/10.1038/s41467-020-20007-4 |
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