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Knockdown of USP8 Inhibits the Growth of Lung Cancer Cells
PURPOSE: Lung cancer is the deadliest tumor in the world. This study aimed to investigate the effection of USP8 on the proliferation and growth of NSCLC cells. METHODS: The proliferation, migration, invasion, cell cycle progression, and apoptosis of A549 and H1299 cells were evaluated with CCK8, col...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Dove
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7719325/ https://www.ncbi.nlm.nih.gov/pubmed/33293867 http://dx.doi.org/10.2147/IJN.S259191 |
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author | Rong, Zhenhua Zhu, Zongmin Cai, Shihua Zhang, Bingqing |
author_facet | Rong, Zhenhua Zhu, Zongmin Cai, Shihua Zhang, Bingqing |
author_sort | Rong, Zhenhua |
collection | PubMed |
description | PURPOSE: Lung cancer is the deadliest tumor in the world. This study aimed to investigate the effection of USP8 on the proliferation and growth of NSCLC cells. METHODS: The proliferation, migration, invasion, cell cycle progression, and apoptosis of A549 and H1299 cells were evaluated with CCK8, colony formation, scratch, transwell, and flow cytometry experiments. Furthermore, the expression of cell cycle- and apoptosis-related proteins was detected by western blot. RESULTS: Knockdown of USP8 inhibited the proliferation, migration, invasion, and cell cycle progression of A549 and H1299 cells, and promoted the apoptosis. The results of western blot indicated that knockdown of USP8 down-regulated the expression of Cyclin D1, CDK4, CDK6, p-AKT, and Bcl2, and up-regulated the expression of Bax. CONCLUSION: Knockdown of USP8 inhibited the proliferation of human lung cancer cells by regulating cell cycle- and apoptosis-related proteins. USP8 may be a therapeutic target for lung cancer. |
format | Online Article Text |
id | pubmed-7719325 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Dove |
record_format | MEDLINE/PubMed |
spelling | pubmed-77193252020-12-07 Knockdown of USP8 Inhibits the Growth of Lung Cancer Cells Rong, Zhenhua Zhu, Zongmin Cai, Shihua Zhang, Bingqing Cancer Manag Res Original Research PURPOSE: Lung cancer is the deadliest tumor in the world. This study aimed to investigate the effection of USP8 on the proliferation and growth of NSCLC cells. METHODS: The proliferation, migration, invasion, cell cycle progression, and apoptosis of A549 and H1299 cells were evaluated with CCK8, colony formation, scratch, transwell, and flow cytometry experiments. Furthermore, the expression of cell cycle- and apoptosis-related proteins was detected by western blot. RESULTS: Knockdown of USP8 inhibited the proliferation, migration, invasion, and cell cycle progression of A549 and H1299 cells, and promoted the apoptosis. The results of western blot indicated that knockdown of USP8 down-regulated the expression of Cyclin D1, CDK4, CDK6, p-AKT, and Bcl2, and up-regulated the expression of Bax. CONCLUSION: Knockdown of USP8 inhibited the proliferation of human lung cancer cells by regulating cell cycle- and apoptosis-related proteins. USP8 may be a therapeutic target for lung cancer. Dove 2020-12-02 /pmc/articles/PMC7719325/ /pubmed/33293867 http://dx.doi.org/10.2147/IJN.S259191 Text en © 2020 Rong et al. http://creativecommons.org/licenses/by-nc/3.0/ This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php). |
spellingShingle | Original Research Rong, Zhenhua Zhu, Zongmin Cai, Shihua Zhang, Bingqing Knockdown of USP8 Inhibits the Growth of Lung Cancer Cells |
title | Knockdown of USP8 Inhibits the Growth of Lung Cancer Cells |
title_full | Knockdown of USP8 Inhibits the Growth of Lung Cancer Cells |
title_fullStr | Knockdown of USP8 Inhibits the Growth of Lung Cancer Cells |
title_full_unstemmed | Knockdown of USP8 Inhibits the Growth of Lung Cancer Cells |
title_short | Knockdown of USP8 Inhibits the Growth of Lung Cancer Cells |
title_sort | knockdown of usp8 inhibits the growth of lung cancer cells |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7719325/ https://www.ncbi.nlm.nih.gov/pubmed/33293867 http://dx.doi.org/10.2147/IJN.S259191 |
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