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Quantification of DNA through the NanoDrop Spectrophotometer: Methodological Validation Using Standard Reference Material and Sprague Dawley Rat and Human DNA
This study aimed to validate an analytical method to determine DNA concentration using standard reference material (NIST SRM 2372) and Sprague Dawley rat and human DNA. Microvolumes were used to analyse DNA samples. Linearity showed correlation coefficients higher than R ≥ 0.9950, and the precision...
| Autores principales: | , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Hindawi
2020
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7719535/ https://www.ncbi.nlm.nih.gov/pubmed/33312204 http://dx.doi.org/10.1155/2020/8896738 |
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| author | García-Alegría, Alejandro Monserrat Anduro-Corona, Iván Pérez-Martínez, Cinthia Jhovanna Guadalupe Corella-Madueño, María Alba Rascón-Durán, María Lucila Astiazaran-Garcia, Humberto |
| author_facet | García-Alegría, Alejandro Monserrat Anduro-Corona, Iván Pérez-Martínez, Cinthia Jhovanna Guadalupe Corella-Madueño, María Alba Rascón-Durán, María Lucila Astiazaran-Garcia, Humberto |
| author_sort | García-Alegría, Alejandro Monserrat |
| collection | PubMed |
| description | This study aimed to validate an analytical method to determine DNA concentration using standard reference material (NIST SRM 2372) and Sprague Dawley rat and human DNA. Microvolumes were used to analyse DNA samples. Linearity showed correlation coefficients higher than R ≥ 0.9950, and the precision value was ≤2% CV. Trueness based on bias and the percentage of recovery showed bias values lower than Z-test with a 95% confidence level and a recovery percentage within the range (% Rec = 100% ± 5%), and the stability of the samples was 60 days (2–4°C). |
| format | Online Article Text |
| id | pubmed-7719535 |
| institution | National Center for Biotechnology Information |
| language | English |
| publishDate | 2020 |
| publisher | Hindawi |
| record_format | MEDLINE/PubMed |
| spelling | pubmed-77195352020-12-11 Quantification of DNA through the NanoDrop Spectrophotometer: Methodological Validation Using Standard Reference Material and Sprague Dawley Rat and Human DNA García-Alegría, Alejandro Monserrat Anduro-Corona, Iván Pérez-Martínez, Cinthia Jhovanna Guadalupe Corella-Madueño, María Alba Rascón-Durán, María Lucila Astiazaran-Garcia, Humberto Int J Anal Chem Research Article This study aimed to validate an analytical method to determine DNA concentration using standard reference material (NIST SRM 2372) and Sprague Dawley rat and human DNA. Microvolumes were used to analyse DNA samples. Linearity showed correlation coefficients higher than R ≥ 0.9950, and the precision value was ≤2% CV. Trueness based on bias and the percentage of recovery showed bias values lower than Z-test with a 95% confidence level and a recovery percentage within the range (% Rec = 100% ± 5%), and the stability of the samples was 60 days (2–4°C). Hindawi 2020-11-29 /pmc/articles/PMC7719535/ /pubmed/33312204 http://dx.doi.org/10.1155/2020/8896738 Text en Copyright © 2020 Alejandro Monserrat García-Alegría et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
| spellingShingle | Research Article García-Alegría, Alejandro Monserrat Anduro-Corona, Iván Pérez-Martínez, Cinthia Jhovanna Guadalupe Corella-Madueño, María Alba Rascón-Durán, María Lucila Astiazaran-Garcia, Humberto Quantification of DNA through the NanoDrop Spectrophotometer: Methodological Validation Using Standard Reference Material and Sprague Dawley Rat and Human DNA |
| title | Quantification of DNA through the NanoDrop Spectrophotometer: Methodological Validation Using Standard Reference Material and Sprague Dawley Rat and Human DNA |
| title_full | Quantification of DNA through the NanoDrop Spectrophotometer: Methodological Validation Using Standard Reference Material and Sprague Dawley Rat and Human DNA |
| title_fullStr | Quantification of DNA through the NanoDrop Spectrophotometer: Methodological Validation Using Standard Reference Material and Sprague Dawley Rat and Human DNA |
| title_full_unstemmed | Quantification of DNA through the NanoDrop Spectrophotometer: Methodological Validation Using Standard Reference Material and Sprague Dawley Rat and Human DNA |
| title_short | Quantification of DNA through the NanoDrop Spectrophotometer: Methodological Validation Using Standard Reference Material and Sprague Dawley Rat and Human DNA |
| title_sort | quantification of dna through the nanodrop spectrophotometer: methodological validation using standard reference material and sprague dawley rat and human dna |
| topic | Research Article |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7719535/ https://www.ncbi.nlm.nih.gov/pubmed/33312204 http://dx.doi.org/10.1155/2020/8896738 |
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