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A Novel MicroRNA From the Translated Region of the Giardiavirus rdrp Gene Governs Virus Copy Number in Giardia duodenalis

Giardia duodenalis is an important zoonotic parasite that can cause human and animal diarrhea. Giardiavirus (GLV) is a double-stranded RNA virus in Totiviridae family, which specifically infects trophozoites of the primitive protozoan parasite G. duodenalis. However, the GLV infectious and the patho...

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Autores principales: Gong, Pengtao, Li, Xianhe, Wu, Wei, Cao, Lili, Zhao, Panpan, Li, Xin, Ren, Baoyan, Li, Jianhua, Zhang, Xichen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7719678/
https://www.ncbi.nlm.nih.gov/pubmed/33329426
http://dx.doi.org/10.3389/fmicb.2020.569412
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author Gong, Pengtao
Li, Xianhe
Wu, Wei
Cao, Lili
Zhao, Panpan
Li, Xin
Ren, Baoyan
Li, Jianhua
Zhang, Xichen
author_facet Gong, Pengtao
Li, Xianhe
Wu, Wei
Cao, Lili
Zhao, Panpan
Li, Xin
Ren, Baoyan
Li, Jianhua
Zhang, Xichen
author_sort Gong, Pengtao
collection PubMed
description Giardia duodenalis is an important zoonotic parasite that can cause human and animal diarrhea. Giardiavirus (GLV) is a double-stranded RNA virus in Totiviridae family, which specifically infects trophozoites of the primitive protozoan parasite G. duodenalis. However, the GLV infectious and the pathogenicity of the G. duodenalis still remain to be confirmed. The GLV genome is 6,277 bp, which encodes two proteins (Gag and Gag-Pol). The expression of Gag-Pol protein is regulated by a-1 ribosomal frameshift. In this report, we identified a novel microRNA (GLV miRNA1) from the GLV. Split ligation northern results showed that GLV miRNA1 is a special expression product of GLV, and the precursor was also identified by primer extension. Antisense sequence of the GLV miRNA1 could increase the copy number of virus in G. duodenalis. It suggests that GLV miRNA1 governs the copy number of Giardiavirus in G. duodenalis. Most importantly, the GLV miRNA1 lies at the translated region of the rdrp gene, which is the first case that microRNA locates in the translated region of a known protein. It may be implying a novel phenomenon for miRNA biogenesis.
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spelling pubmed-77196782020-12-15 A Novel MicroRNA From the Translated Region of the Giardiavirus rdrp Gene Governs Virus Copy Number in Giardia duodenalis Gong, Pengtao Li, Xianhe Wu, Wei Cao, Lili Zhao, Panpan Li, Xin Ren, Baoyan Li, Jianhua Zhang, Xichen Front Microbiol Microbiology Giardia duodenalis is an important zoonotic parasite that can cause human and animal diarrhea. Giardiavirus (GLV) is a double-stranded RNA virus in Totiviridae family, which specifically infects trophozoites of the primitive protozoan parasite G. duodenalis. However, the GLV infectious and the pathogenicity of the G. duodenalis still remain to be confirmed. The GLV genome is 6,277 bp, which encodes two proteins (Gag and Gag-Pol). The expression of Gag-Pol protein is regulated by a-1 ribosomal frameshift. In this report, we identified a novel microRNA (GLV miRNA1) from the GLV. Split ligation northern results showed that GLV miRNA1 is a special expression product of GLV, and the precursor was also identified by primer extension. Antisense sequence of the GLV miRNA1 could increase the copy number of virus in G. duodenalis. It suggests that GLV miRNA1 governs the copy number of Giardiavirus in G. duodenalis. Most importantly, the GLV miRNA1 lies at the translated region of the rdrp gene, which is the first case that microRNA locates in the translated region of a known protein. It may be implying a novel phenomenon for miRNA biogenesis. Frontiers Media S.A. 2020-11-23 /pmc/articles/PMC7719678/ /pubmed/33329426 http://dx.doi.org/10.3389/fmicb.2020.569412 Text en Copyright © 2020 Gong, Li, Wu, Cao, Zhao, Li, Ren, Li and Zhang. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Gong, Pengtao
Li, Xianhe
Wu, Wei
Cao, Lili
Zhao, Panpan
Li, Xin
Ren, Baoyan
Li, Jianhua
Zhang, Xichen
A Novel MicroRNA From the Translated Region of the Giardiavirus rdrp Gene Governs Virus Copy Number in Giardia duodenalis
title A Novel MicroRNA From the Translated Region of the Giardiavirus rdrp Gene Governs Virus Copy Number in Giardia duodenalis
title_full A Novel MicroRNA From the Translated Region of the Giardiavirus rdrp Gene Governs Virus Copy Number in Giardia duodenalis
title_fullStr A Novel MicroRNA From the Translated Region of the Giardiavirus rdrp Gene Governs Virus Copy Number in Giardia duodenalis
title_full_unstemmed A Novel MicroRNA From the Translated Region of the Giardiavirus rdrp Gene Governs Virus Copy Number in Giardia duodenalis
title_short A Novel MicroRNA From the Translated Region of the Giardiavirus rdrp Gene Governs Virus Copy Number in Giardia duodenalis
title_sort novel microrna from the translated region of the giardiavirus rdrp gene governs virus copy number in giardia duodenalis
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7719678/
https://www.ncbi.nlm.nih.gov/pubmed/33329426
http://dx.doi.org/10.3389/fmicb.2020.569412
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