Cargando…
Analyses of Avascular Mutants Reveal Unique Transcriptomic Signature of Non-conventional Endothelial Cells
Endothelial cells appear to emerge from diverse progenitors. However, to which extent their developmental origin contributes to define their cellular and molecular characteristics remains largely unknown. Here, we report that a subset of endothelial cells that emerge from the tailbud possess unique...
Autores principales: | , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7719722/ https://www.ncbi.nlm.nih.gov/pubmed/33330468 http://dx.doi.org/10.3389/fcell.2020.589717 |
_version_ | 1783619734606970880 |
---|---|
author | Pak, Boryeong Schmitt, Christopher E. Choi, Woosoung Kim, Jun-Dae Han, Orjin Alsiö, Jessica Jung, Da-Woon Williams, Darren R. Coppieters, Wouter Stainier, Didier Y. R. Jin, Suk-Won |
author_facet | Pak, Boryeong Schmitt, Christopher E. Choi, Woosoung Kim, Jun-Dae Han, Orjin Alsiö, Jessica Jung, Da-Woon Williams, Darren R. Coppieters, Wouter Stainier, Didier Y. R. Jin, Suk-Won |
author_sort | Pak, Boryeong |
collection | PubMed |
description | Endothelial cells appear to emerge from diverse progenitors. However, to which extent their developmental origin contributes to define their cellular and molecular characteristics remains largely unknown. Here, we report that a subset of endothelial cells that emerge from the tailbud possess unique molecular characteristics that set them apart from stereotypical lateral plate mesoderm (LPM)-derived endothelial cells. Lineage tracing shows that these tailbud-derived endothelial cells arise at mid-somitogenesis stages, and surprisingly do not require Npas4l or Etsrp function, indicating that they have distinct spatiotemporal origins and are regulated by distinct molecular mechanisms. Microarray and single cell RNA-seq analyses reveal that somitogenesis- and neurogenesis-associated transcripts are over-represented in these tailbud-derived endothelial cells, suggesting that they possess a unique transcriptomic signature. Taken together, our results further reveal the diversity of endothelial cells with respect to their developmental origin and molecular properties, and provide compelling evidence that the molecular characteristics of endothelial cells may reflect their distinct developmental history. |
format | Online Article Text |
id | pubmed-7719722 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-77197222020-12-15 Analyses of Avascular Mutants Reveal Unique Transcriptomic Signature of Non-conventional Endothelial Cells Pak, Boryeong Schmitt, Christopher E. Choi, Woosoung Kim, Jun-Dae Han, Orjin Alsiö, Jessica Jung, Da-Woon Williams, Darren R. Coppieters, Wouter Stainier, Didier Y. R. Jin, Suk-Won Front Cell Dev Biol Cell and Developmental Biology Endothelial cells appear to emerge from diverse progenitors. However, to which extent their developmental origin contributes to define their cellular and molecular characteristics remains largely unknown. Here, we report that a subset of endothelial cells that emerge from the tailbud possess unique molecular characteristics that set them apart from stereotypical lateral plate mesoderm (LPM)-derived endothelial cells. Lineage tracing shows that these tailbud-derived endothelial cells arise at mid-somitogenesis stages, and surprisingly do not require Npas4l or Etsrp function, indicating that they have distinct spatiotemporal origins and are regulated by distinct molecular mechanisms. Microarray and single cell RNA-seq analyses reveal that somitogenesis- and neurogenesis-associated transcripts are over-represented in these tailbud-derived endothelial cells, suggesting that they possess a unique transcriptomic signature. Taken together, our results further reveal the diversity of endothelial cells with respect to their developmental origin and molecular properties, and provide compelling evidence that the molecular characteristics of endothelial cells may reflect their distinct developmental history. Frontiers Media S.A. 2020-11-23 /pmc/articles/PMC7719722/ /pubmed/33330468 http://dx.doi.org/10.3389/fcell.2020.589717 Text en Copyright © 2020 Pak, Schmitt, Choi, Kim, Han, Alsiö, Jung, Williams, Coppieters, Stainier and Jin. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Cell and Developmental Biology Pak, Boryeong Schmitt, Christopher E. Choi, Woosoung Kim, Jun-Dae Han, Orjin Alsiö, Jessica Jung, Da-Woon Williams, Darren R. Coppieters, Wouter Stainier, Didier Y. R. Jin, Suk-Won Analyses of Avascular Mutants Reveal Unique Transcriptomic Signature of Non-conventional Endothelial Cells |
title | Analyses of Avascular Mutants Reveal Unique Transcriptomic Signature of Non-conventional Endothelial Cells |
title_full | Analyses of Avascular Mutants Reveal Unique Transcriptomic Signature of Non-conventional Endothelial Cells |
title_fullStr | Analyses of Avascular Mutants Reveal Unique Transcriptomic Signature of Non-conventional Endothelial Cells |
title_full_unstemmed | Analyses of Avascular Mutants Reveal Unique Transcriptomic Signature of Non-conventional Endothelial Cells |
title_short | Analyses of Avascular Mutants Reveal Unique Transcriptomic Signature of Non-conventional Endothelial Cells |
title_sort | analyses of avascular mutants reveal unique transcriptomic signature of non-conventional endothelial cells |
topic | Cell and Developmental Biology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7719722/ https://www.ncbi.nlm.nih.gov/pubmed/33330468 http://dx.doi.org/10.3389/fcell.2020.589717 |
work_keys_str_mv | AT pakboryeong analysesofavascularmutantsrevealuniquetranscriptomicsignatureofnonconventionalendothelialcells AT schmittchristophere analysesofavascularmutantsrevealuniquetranscriptomicsignatureofnonconventionalendothelialcells AT choiwoosoung analysesofavascularmutantsrevealuniquetranscriptomicsignatureofnonconventionalendothelialcells AT kimjundae analysesofavascularmutantsrevealuniquetranscriptomicsignatureofnonconventionalendothelialcells AT hanorjin analysesofavascularmutantsrevealuniquetranscriptomicsignatureofnonconventionalendothelialcells AT alsiojessica analysesofavascularmutantsrevealuniquetranscriptomicsignatureofnonconventionalendothelialcells AT jungdawoon analysesofavascularmutantsrevealuniquetranscriptomicsignatureofnonconventionalendothelialcells AT williamsdarrenr analysesofavascularmutantsrevealuniquetranscriptomicsignatureofnonconventionalendothelialcells AT coppieterswouter analysesofavascularmutantsrevealuniquetranscriptomicsignatureofnonconventionalendothelialcells AT stainierdidieryr analysesofavascularmutantsrevealuniquetranscriptomicsignatureofnonconventionalendothelialcells AT jinsukwon analysesofavascularmutantsrevealuniquetranscriptomicsignatureofnonconventionalendothelialcells |