Cargando…

Targeting laryngeal cancer cells with 5-fluorouracil and curcumin using mesoporous silica nanoparticles

OBJECTIVE: To explore the inhibitory and synergistic effects of 5-fluorouracil and curcumin on Hep-2 laryngeal cancer cells and clarify the effect of mesoporous silica nanoparticles as drug carriers. METHODS: The inhibitory effects of 5-fluorouracil and curcumin on Hep-2 cells were detected using th...

Descripción completa

Detalles Bibliográficos
Autores principales: Wang, Ding, Yu, Dan, Liu, Xueshibojie, Wang, Qian, Chen, Xuyang, Hu, Xindan, Wang, Qiong, Jin, Chunshun, Wen, Lianji, Zhang, Ling
Formato: Online Artículo Texto
Lenguaje:English
Publicado: SAGE Publications 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7720313/
https://www.ncbi.nlm.nih.gov/pubmed/33267716
http://dx.doi.org/10.1177/1533033820962114
_version_ 1783619825712496640
author Wang, Ding
Yu, Dan
Liu, Xueshibojie
Wang, Qian
Chen, Xuyang
Hu, Xindan
Wang, Qiong
Jin, Chunshun
Wen, Lianji
Zhang, Ling
author_facet Wang, Ding
Yu, Dan
Liu, Xueshibojie
Wang, Qian
Chen, Xuyang
Hu, Xindan
Wang, Qiong
Jin, Chunshun
Wen, Lianji
Zhang, Ling
author_sort Wang, Ding
collection PubMed
description OBJECTIVE: To explore the inhibitory and synergistic effects of 5-fluorouracil and curcumin on Hep-2 laryngeal cancer cells and clarify the effect of mesoporous silica nanoparticles as drug carriers. METHODS: The inhibitory effects of 5-fluorouracil and curcumin on Hep-2 cells were detected using the CCK-8 assay. CompuSyn was used to calculate the synergistic effect of the 2 drugs. Flow cytometry was used to detect apoptosis and cell cycle arrest induced by 5-fluorouracil and curcumin. The drugs were loaded into mesoporous nanoparticles. Western blotting was used to detect the expression of related proteins after treatment. The growth of subcutaneous tumors in BALB/c nude after the intraperitoneal injection with drug-loaded mesoporous silica nanoparticles was recorded. RESULTS: 5-Fluorouracil and curcumin synergistically induced apoptosis and cell cycle arrest in Hep-2 cells. Mesoporous silica nanoparticles as drug carriers enhanced the therapeutic effects of 5-fluorouracil and curcumin. CONCLUSIONS: Mesoporous silica nanoparticles are expected to be effective drug carriers that enhance the synergistic effects of 5-fluorouracil and curcumin on laryngeal cancer.
format Online
Article
Text
id pubmed-7720313
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher SAGE Publications
record_format MEDLINE/PubMed
spelling pubmed-77203132020-12-15 Targeting laryngeal cancer cells with 5-fluorouracil and curcumin using mesoporous silica nanoparticles Wang, Ding Yu, Dan Liu, Xueshibojie Wang, Qian Chen, Xuyang Hu, Xindan Wang, Qiong Jin, Chunshun Wen, Lianji Zhang, Ling Technol Cancer Res Treat Original Article OBJECTIVE: To explore the inhibitory and synergistic effects of 5-fluorouracil and curcumin on Hep-2 laryngeal cancer cells and clarify the effect of mesoporous silica nanoparticles as drug carriers. METHODS: The inhibitory effects of 5-fluorouracil and curcumin on Hep-2 cells were detected using the CCK-8 assay. CompuSyn was used to calculate the synergistic effect of the 2 drugs. Flow cytometry was used to detect apoptosis and cell cycle arrest induced by 5-fluorouracil and curcumin. The drugs were loaded into mesoporous nanoparticles. Western blotting was used to detect the expression of related proteins after treatment. The growth of subcutaneous tumors in BALB/c nude after the intraperitoneal injection with drug-loaded mesoporous silica nanoparticles was recorded. RESULTS: 5-Fluorouracil and curcumin synergistically induced apoptosis and cell cycle arrest in Hep-2 cells. Mesoporous silica nanoparticles as drug carriers enhanced the therapeutic effects of 5-fluorouracil and curcumin. CONCLUSIONS: Mesoporous silica nanoparticles are expected to be effective drug carriers that enhance the synergistic effects of 5-fluorouracil and curcumin on laryngeal cancer. SAGE Publications 2020-12-03 /pmc/articles/PMC7720313/ /pubmed/33267716 http://dx.doi.org/10.1177/1533033820962114 Text en © The Author(s) 2020 https://creativecommons.org/licenses/by-nc/4.0/ This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).
spellingShingle Original Article
Wang, Ding
Yu, Dan
Liu, Xueshibojie
Wang, Qian
Chen, Xuyang
Hu, Xindan
Wang, Qiong
Jin, Chunshun
Wen, Lianji
Zhang, Ling
Targeting laryngeal cancer cells with 5-fluorouracil and curcumin using mesoporous silica nanoparticles
title Targeting laryngeal cancer cells with 5-fluorouracil and curcumin using mesoporous silica nanoparticles
title_full Targeting laryngeal cancer cells with 5-fluorouracil and curcumin using mesoporous silica nanoparticles
title_fullStr Targeting laryngeal cancer cells with 5-fluorouracil and curcumin using mesoporous silica nanoparticles
title_full_unstemmed Targeting laryngeal cancer cells with 5-fluorouracil and curcumin using mesoporous silica nanoparticles
title_short Targeting laryngeal cancer cells with 5-fluorouracil and curcumin using mesoporous silica nanoparticles
title_sort targeting laryngeal cancer cells with 5-fluorouracil and curcumin using mesoporous silica nanoparticles
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7720313/
https://www.ncbi.nlm.nih.gov/pubmed/33267716
http://dx.doi.org/10.1177/1533033820962114
work_keys_str_mv AT wangding targetinglaryngealcancercellswith5fluorouracilandcurcuminusingmesoporoussilicananoparticles
AT yudan targetinglaryngealcancercellswith5fluorouracilandcurcuminusingmesoporoussilicananoparticles
AT liuxueshibojie targetinglaryngealcancercellswith5fluorouracilandcurcuminusingmesoporoussilicananoparticles
AT wangqian targetinglaryngealcancercellswith5fluorouracilandcurcuminusingmesoporoussilicananoparticles
AT chenxuyang targetinglaryngealcancercellswith5fluorouracilandcurcuminusingmesoporoussilicananoparticles
AT huxindan targetinglaryngealcancercellswith5fluorouracilandcurcuminusingmesoporoussilicananoparticles
AT wangqiong targetinglaryngealcancercellswith5fluorouracilandcurcuminusingmesoporoussilicananoparticles
AT jinchunshun targetinglaryngealcancercellswith5fluorouracilandcurcuminusingmesoporoussilicananoparticles
AT wenlianji targetinglaryngealcancercellswith5fluorouracilandcurcuminusingmesoporoussilicananoparticles
AT zhangling targetinglaryngealcancercellswith5fluorouracilandcurcuminusingmesoporoussilicananoparticles