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Targeting laryngeal cancer cells with 5-fluorouracil and curcumin using mesoporous silica nanoparticles
OBJECTIVE: To explore the inhibitory and synergistic effects of 5-fluorouracil and curcumin on Hep-2 laryngeal cancer cells and clarify the effect of mesoporous silica nanoparticles as drug carriers. METHODS: The inhibitory effects of 5-fluorouracil and curcumin on Hep-2 cells were detected using th...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
SAGE Publications
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7720313/ https://www.ncbi.nlm.nih.gov/pubmed/33267716 http://dx.doi.org/10.1177/1533033820962114 |
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author | Wang, Ding Yu, Dan Liu, Xueshibojie Wang, Qian Chen, Xuyang Hu, Xindan Wang, Qiong Jin, Chunshun Wen, Lianji Zhang, Ling |
author_facet | Wang, Ding Yu, Dan Liu, Xueshibojie Wang, Qian Chen, Xuyang Hu, Xindan Wang, Qiong Jin, Chunshun Wen, Lianji Zhang, Ling |
author_sort | Wang, Ding |
collection | PubMed |
description | OBJECTIVE: To explore the inhibitory and synergistic effects of 5-fluorouracil and curcumin on Hep-2 laryngeal cancer cells and clarify the effect of mesoporous silica nanoparticles as drug carriers. METHODS: The inhibitory effects of 5-fluorouracil and curcumin on Hep-2 cells were detected using the CCK-8 assay. CompuSyn was used to calculate the synergistic effect of the 2 drugs. Flow cytometry was used to detect apoptosis and cell cycle arrest induced by 5-fluorouracil and curcumin. The drugs were loaded into mesoporous nanoparticles. Western blotting was used to detect the expression of related proteins after treatment. The growth of subcutaneous tumors in BALB/c nude after the intraperitoneal injection with drug-loaded mesoporous silica nanoparticles was recorded. RESULTS: 5-Fluorouracil and curcumin synergistically induced apoptosis and cell cycle arrest in Hep-2 cells. Mesoporous silica nanoparticles as drug carriers enhanced the therapeutic effects of 5-fluorouracil and curcumin. CONCLUSIONS: Mesoporous silica nanoparticles are expected to be effective drug carriers that enhance the synergistic effects of 5-fluorouracil and curcumin on laryngeal cancer. |
format | Online Article Text |
id | pubmed-7720313 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | SAGE Publications |
record_format | MEDLINE/PubMed |
spelling | pubmed-77203132020-12-15 Targeting laryngeal cancer cells with 5-fluorouracil and curcumin using mesoporous silica nanoparticles Wang, Ding Yu, Dan Liu, Xueshibojie Wang, Qian Chen, Xuyang Hu, Xindan Wang, Qiong Jin, Chunshun Wen, Lianji Zhang, Ling Technol Cancer Res Treat Original Article OBJECTIVE: To explore the inhibitory and synergistic effects of 5-fluorouracil and curcumin on Hep-2 laryngeal cancer cells and clarify the effect of mesoporous silica nanoparticles as drug carriers. METHODS: The inhibitory effects of 5-fluorouracil and curcumin on Hep-2 cells were detected using the CCK-8 assay. CompuSyn was used to calculate the synergistic effect of the 2 drugs. Flow cytometry was used to detect apoptosis and cell cycle arrest induced by 5-fluorouracil and curcumin. The drugs were loaded into mesoporous nanoparticles. Western blotting was used to detect the expression of related proteins after treatment. The growth of subcutaneous tumors in BALB/c nude after the intraperitoneal injection with drug-loaded mesoporous silica nanoparticles was recorded. RESULTS: 5-Fluorouracil and curcumin synergistically induced apoptosis and cell cycle arrest in Hep-2 cells. Mesoporous silica nanoparticles as drug carriers enhanced the therapeutic effects of 5-fluorouracil and curcumin. CONCLUSIONS: Mesoporous silica nanoparticles are expected to be effective drug carriers that enhance the synergistic effects of 5-fluorouracil and curcumin on laryngeal cancer. SAGE Publications 2020-12-03 /pmc/articles/PMC7720313/ /pubmed/33267716 http://dx.doi.org/10.1177/1533033820962114 Text en © The Author(s) 2020 https://creativecommons.org/licenses/by-nc/4.0/ This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage). |
spellingShingle | Original Article Wang, Ding Yu, Dan Liu, Xueshibojie Wang, Qian Chen, Xuyang Hu, Xindan Wang, Qiong Jin, Chunshun Wen, Lianji Zhang, Ling Targeting laryngeal cancer cells with 5-fluorouracil and curcumin using mesoporous silica nanoparticles |
title | Targeting laryngeal cancer cells with 5-fluorouracil and curcumin using mesoporous silica nanoparticles |
title_full | Targeting laryngeal cancer cells with 5-fluorouracil and curcumin using mesoporous silica nanoparticles |
title_fullStr | Targeting laryngeal cancer cells with 5-fluorouracil and curcumin using mesoporous silica nanoparticles |
title_full_unstemmed | Targeting laryngeal cancer cells with 5-fluorouracil and curcumin using mesoporous silica nanoparticles |
title_short | Targeting laryngeal cancer cells with 5-fluorouracil and curcumin using mesoporous silica nanoparticles |
title_sort | targeting laryngeal cancer cells with 5-fluorouracil and curcumin using mesoporous silica nanoparticles |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7720313/ https://www.ncbi.nlm.nih.gov/pubmed/33267716 http://dx.doi.org/10.1177/1533033820962114 |
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