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Label-free imaging of lipid-rich biological tissues by mid-infrared photoacoustic microscopy

Significance: Mid-infrared (IR) imaging based on the vibrational transition of biomolecules provides good chemical-specific contrast in label-free imaging of biology tissues, making it a popular tool in both biomedical studies and clinical applications. However, the current technology typically requ...

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Autores principales: He, Yun, Shi, Junhui, Pleitez, Miguel A., Maslov, Konstantin, Wagenaar, Daniel A., Wang, Lihong V.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Society of Photo-Optical Instrumentation Engineers 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7720905/
https://www.ncbi.nlm.nih.gov/pubmed/33118344
http://dx.doi.org/10.1117/1.JBO.25.10.106506
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author He, Yun
Shi, Junhui
Pleitez, Miguel A.
Maslov, Konstantin
Wagenaar, Daniel A.
Wang, Lihong V.
author_facet He, Yun
Shi, Junhui
Pleitez, Miguel A.
Maslov, Konstantin
Wagenaar, Daniel A.
Wang, Lihong V.
author_sort He, Yun
collection PubMed
description Significance: Mid-infrared (IR) imaging based on the vibrational transition of biomolecules provides good chemical-specific contrast in label-free imaging of biology tissues, making it a popular tool in both biomedical studies and clinical applications. However, the current technology typically requires thin and dried or extremely flat samples, whose complicated processing limits this technology’s broader translation. Aim: To address this issue, we report mid-IR photoacoustic microscopy (PAM), which can readily work with fresh and thick tissue samples, even when they have rough surfaces. Approach: We developed a transmission-mode mid-IR PAM system employing an optical parametric oscillation laser operating in the wavelength range from 2.5 to [Formula: see text]. Due to its high sensitivity to optical absorption and the low ultrasonic attenuation of tissue, our PAM achieved greater probing depth than Fourier transform IR spectroscopy, thus enabling imaging fresh and thick tissue samples with rough surfaces. Results: In our spectroscopy study, the [Formula: see text] symmetric stretching at [Formula: see text] (3508 nm) was found to be an excellent source of endogenous contrast for lipids. At this wavenumber, we demonstrated label-free imaging of the lipid composition in fresh, manually cut, and unprocessed tissue sections of up to 3-mm thickness. Conclusions: Our technology requires no time-consuming sample preparation procedure and has great potential in both fast clinical histological analysis and fundamental biological studies.
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spelling pubmed-77209052020-12-09 Label-free imaging of lipid-rich biological tissues by mid-infrared photoacoustic microscopy He, Yun Shi, Junhui Pleitez, Miguel A. Maslov, Konstantin Wagenaar, Daniel A. Wang, Lihong V. J Biomed Opt Microscopy Significance: Mid-infrared (IR) imaging based on the vibrational transition of biomolecules provides good chemical-specific contrast in label-free imaging of biology tissues, making it a popular tool in both biomedical studies and clinical applications. However, the current technology typically requires thin and dried or extremely flat samples, whose complicated processing limits this technology’s broader translation. Aim: To address this issue, we report mid-IR photoacoustic microscopy (PAM), which can readily work with fresh and thick tissue samples, even when they have rough surfaces. Approach: We developed a transmission-mode mid-IR PAM system employing an optical parametric oscillation laser operating in the wavelength range from 2.5 to [Formula: see text]. Due to its high sensitivity to optical absorption and the low ultrasonic attenuation of tissue, our PAM achieved greater probing depth than Fourier transform IR spectroscopy, thus enabling imaging fresh and thick tissue samples with rough surfaces. Results: In our spectroscopy study, the [Formula: see text] symmetric stretching at [Formula: see text] (3508 nm) was found to be an excellent source of endogenous contrast for lipids. At this wavenumber, we demonstrated label-free imaging of the lipid composition in fresh, manually cut, and unprocessed tissue sections of up to 3-mm thickness. Conclusions: Our technology requires no time-consuming sample preparation procedure and has great potential in both fast clinical histological analysis and fundamental biological studies. Society of Photo-Optical Instrumentation Engineers 2020-10-28 2020-10 /pmc/articles/PMC7720905/ /pubmed/33118344 http://dx.doi.org/10.1117/1.JBO.25.10.106506 Text en © 2020 The Authors https://creativecommons.org/licenses/by/4.0/ Published by SPIE under a Creative Commons Attribution 4.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.
spellingShingle Microscopy
He, Yun
Shi, Junhui
Pleitez, Miguel A.
Maslov, Konstantin
Wagenaar, Daniel A.
Wang, Lihong V.
Label-free imaging of lipid-rich biological tissues by mid-infrared photoacoustic microscopy
title Label-free imaging of lipid-rich biological tissues by mid-infrared photoacoustic microscopy
title_full Label-free imaging of lipid-rich biological tissues by mid-infrared photoacoustic microscopy
title_fullStr Label-free imaging of lipid-rich biological tissues by mid-infrared photoacoustic microscopy
title_full_unstemmed Label-free imaging of lipid-rich biological tissues by mid-infrared photoacoustic microscopy
title_short Label-free imaging of lipid-rich biological tissues by mid-infrared photoacoustic microscopy
title_sort label-free imaging of lipid-rich biological tissues by mid-infrared photoacoustic microscopy
topic Microscopy
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7720905/
https://www.ncbi.nlm.nih.gov/pubmed/33118344
http://dx.doi.org/10.1117/1.JBO.25.10.106506
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