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High-speed axially swept light sheet microscopy using a linear MEMS phased array for isotropic resolution

Significance: Axially swept light sheet microscopy is used for deconvolution-free, high-resolution 3D imaging, but usually the axial scan mechanism reduces the top imaging speed. Phased arrays (PAs) for axial scanning enable both high resolution and high speed. Aim: A high-speed PA with an update ra...

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Detalles Bibliográficos
Autores principales: Landry, Joseph, Hamann, Stephen, Solgaard, Olav
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Society of Photo-Optical Instrumentation Engineers 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7720907/
https://www.ncbi.nlm.nih.gov/pubmed/33098281
http://dx.doi.org/10.1117/1.JBO.25.10.106504
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author Landry, Joseph
Hamann, Stephen
Solgaard, Olav
author_facet Landry, Joseph
Hamann, Stephen
Solgaard, Olav
author_sort Landry, Joseph
collection PubMed
description Significance: Axially swept light sheet microscopy is used for deconvolution-free, high-resolution 3D imaging, but usually the axial scan mechanism reduces the top imaging speed. Phased arrays (PAs) for axial scanning enable both high resolution and high speed. Aim: A high-speed PA with an update rate faster than the camera row read time is used to track the rolling shutter at camera-limited rates. Approach: The point spread function is evaluated to ensure sub-micron isotropic resolution, and the technique is demonstrated on a live Drosophila embryo. Results: Isotropic resolution is shown down to [Formula: see text] in all three spatial dimensions. With an update rate of [Formula: see text] , the PA tracks the camera sensor rolling shutter at camera-limited rates. Features in the Drosophila embryo are resolved clearly compared with the equivalent static light sheet case. The random-access nature of the PA enables a camera sensor readout in the same direction for each frame to maintain even temporal sampling in image sequences with no speed loss. Conclusions: Use of PAs is compatible with axially swept light sheet microscopy and offers significant improvements in speed.
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spelling pubmed-77209072020-12-09 High-speed axially swept light sheet microscopy using a linear MEMS phased array for isotropic resolution Landry, Joseph Hamann, Stephen Solgaard, Olav J Biomed Opt Microscopy Significance: Axially swept light sheet microscopy is used for deconvolution-free, high-resolution 3D imaging, but usually the axial scan mechanism reduces the top imaging speed. Phased arrays (PAs) for axial scanning enable both high resolution and high speed. Aim: A high-speed PA with an update rate faster than the camera row read time is used to track the rolling shutter at camera-limited rates. Approach: The point spread function is evaluated to ensure sub-micron isotropic resolution, and the technique is demonstrated on a live Drosophila embryo. Results: Isotropic resolution is shown down to [Formula: see text] in all three spatial dimensions. With an update rate of [Formula: see text] , the PA tracks the camera sensor rolling shutter at camera-limited rates. Features in the Drosophila embryo are resolved clearly compared with the equivalent static light sheet case. The random-access nature of the PA enables a camera sensor readout in the same direction for each frame to maintain even temporal sampling in image sequences with no speed loss. Conclusions: Use of PAs is compatible with axially swept light sheet microscopy and offers significant improvements in speed. Society of Photo-Optical Instrumentation Engineers 2020-10-23 2020-10 /pmc/articles/PMC7720907/ /pubmed/33098281 http://dx.doi.org/10.1117/1.JBO.25.10.106504 Text en © 2020 The Authors https://creativecommons.org/licenses/by/4.0/ Published by SPIE under a Creative Commons Attribution 4.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.
spellingShingle Microscopy
Landry, Joseph
Hamann, Stephen
Solgaard, Olav
High-speed axially swept light sheet microscopy using a linear MEMS phased array for isotropic resolution
title High-speed axially swept light sheet microscopy using a linear MEMS phased array for isotropic resolution
title_full High-speed axially swept light sheet microscopy using a linear MEMS phased array for isotropic resolution
title_fullStr High-speed axially swept light sheet microscopy using a linear MEMS phased array for isotropic resolution
title_full_unstemmed High-speed axially swept light sheet microscopy using a linear MEMS phased array for isotropic resolution
title_short High-speed axially swept light sheet microscopy using a linear MEMS phased array for isotropic resolution
title_sort high-speed axially swept light sheet microscopy using a linear mems phased array for isotropic resolution
topic Microscopy
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7720907/
https://www.ncbi.nlm.nih.gov/pubmed/33098281
http://dx.doi.org/10.1117/1.JBO.25.10.106504
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