The mitochondrial genome analysis of Paragnetina indentata (Plecoptera: Perlidae)

In this study, the entire mitochondrial genome of Paragnetina indentata was sequenced. The circular mitochondrial genome was the first mitochondrial genome representing the genus Paragnetina, which consists of 13 protein-coding genes (PCGs), 2 ribosomal RNA genes, 22 transfer RNA genes, and 1 control region with the length of 15,885 bp. In the complete sequence, the A + T content for 64.1% (A for 33.6%, T for 30.5%, C for 23.3%, and G for 12.7%). Among the 13 PCGs, 11 start codons is ATN and the start codon of ND1, ND2, and ND5 genes is TTG, GTG, and GTG, respectively. In addition, 11 of the PCGs used conservative termination codon TAA or TAG, except for COII and ND5 which terminated by the single T. By using the Bayesian inference (BI) and maximum-likelihood (ML) methods, the phylogenetic relationship showed that P. indentata was closely related to Togoperla sp. and the species of Perlinae were clustered in a clade.