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In vitro synergy of isavuconazole in combination with colistin against Candida auris
The in vitro interactions of isavuconazole with colistin were evaluated against 15 clinical Candida auris isolates by a microdilution checkerboard technique based on the EUCAST reference method for antifungal susceptibility testing and by agar diffusion using isavuconazole gradient concentration str...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7722718/ https://www.ncbi.nlm.nih.gov/pubmed/33293607 http://dx.doi.org/10.1038/s41598-020-78588-5 |
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author | Schwarz, Patrick Bidaud, Anne-Laure Dannaoui, Eric |
author_facet | Schwarz, Patrick Bidaud, Anne-Laure Dannaoui, Eric |
author_sort | Schwarz, Patrick |
collection | PubMed |
description | The in vitro interactions of isavuconazole with colistin were evaluated against 15 clinical Candida auris isolates by a microdilution checkerboard technique based on the EUCAST reference method for antifungal susceptibility testing and by agar diffusion using isavuconazole gradient concentration strips with or without colistin incorporated RPMI agar. Interpretation of the checkerboard results was done by the fractional inhibitory concentration index and by response surface analysis based on the Bliss model. By checkerboard, combination was synergistic for 93% of the isolates when interpretation of the data was done by fractional inhibitory concentration index, and for 80% of the isolates by response surface analysis interpretation. By agar diffusion test, although all MICs in combination decreased compared to isavuconazole alone, only 13% of the isolates met the definition of synergy. Essential agreement of EUCAST and gradient concentration strip MICs at +/− 2 log(2) dilutions was 93.3%. Antagonistic interactions were never observed for any technique or interpretation model used. |
format | Online Article Text |
id | pubmed-7722718 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-77227182020-12-09 In vitro synergy of isavuconazole in combination with colistin against Candida auris Schwarz, Patrick Bidaud, Anne-Laure Dannaoui, Eric Sci Rep Article The in vitro interactions of isavuconazole with colistin were evaluated against 15 clinical Candida auris isolates by a microdilution checkerboard technique based on the EUCAST reference method for antifungal susceptibility testing and by agar diffusion using isavuconazole gradient concentration strips with or without colistin incorporated RPMI agar. Interpretation of the checkerboard results was done by the fractional inhibitory concentration index and by response surface analysis based on the Bliss model. By checkerboard, combination was synergistic for 93% of the isolates when interpretation of the data was done by fractional inhibitory concentration index, and for 80% of the isolates by response surface analysis interpretation. By agar diffusion test, although all MICs in combination decreased compared to isavuconazole alone, only 13% of the isolates met the definition of synergy. Essential agreement of EUCAST and gradient concentration strip MICs at +/− 2 log(2) dilutions was 93.3%. Antagonistic interactions were never observed for any technique or interpretation model used. Nature Publishing Group UK 2020-12-08 /pmc/articles/PMC7722718/ /pubmed/33293607 http://dx.doi.org/10.1038/s41598-020-78588-5 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Schwarz, Patrick Bidaud, Anne-Laure Dannaoui, Eric In vitro synergy of isavuconazole in combination with colistin against Candida auris |
title | In vitro synergy of isavuconazole in combination with colistin against Candida auris |
title_full | In vitro synergy of isavuconazole in combination with colistin against Candida auris |
title_fullStr | In vitro synergy of isavuconazole in combination with colistin against Candida auris |
title_full_unstemmed | In vitro synergy of isavuconazole in combination with colistin against Candida auris |
title_short | In vitro synergy of isavuconazole in combination with colistin against Candida auris |
title_sort | in vitro synergy of isavuconazole in combination with colistin against candida auris |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7722718/ https://www.ncbi.nlm.nih.gov/pubmed/33293607 http://dx.doi.org/10.1038/s41598-020-78588-5 |
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