Structure of native glycolipoprotein filaments in honeybee royal jelly

Royal jelly (RJ) is produced by honeybees (Apis mellifera) as nutrition during larval development. The high viscosity of RJ originates from high concentrations of long lipoprotein filaments that include the glycosylated major royal jelly protein 1 (MRJP1), the small protein apisimin and insect lipid...

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Autores principales: Mattei, Simone, Ban, Arvid, Picenoni, Armin, Leibundgut, Marc, Glockshuber, Rudi, Boehringer, Daniel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7722742/
https://www.ncbi.nlm.nih.gov/pubmed/33293513
http://dx.doi.org/10.1038/s41467-020-20135-x
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author Mattei, Simone
Ban, Arvid
Picenoni, Armin
Leibundgut, Marc
Glockshuber, Rudi
Boehringer, Daniel
author_facet Mattei, Simone
Ban, Arvid
Picenoni, Armin
Leibundgut, Marc
Glockshuber, Rudi
Boehringer, Daniel
author_sort Mattei, Simone
collection PubMed
description Royal jelly (RJ) is produced by honeybees (Apis mellifera) as nutrition during larval development. The high viscosity of RJ originates from high concentrations of long lipoprotein filaments that include the glycosylated major royal jelly protein 1 (MRJP1), the small protein apisimin and insect lipids. Using cryo-electron microscopy we reveal the architecture and the composition of RJ filaments, in which the MRJP1 forms the outer shell of the assembly, surrounding stacked apisimin tetramers harbouring tightly packed lipids in the centre. The structural data rationalize the pH-dependent disassembly of RJ filaments in the gut of the larvae.
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spelling pubmed-77227422020-12-11 Structure of native glycolipoprotein filaments in honeybee royal jelly Mattei, Simone Ban, Arvid Picenoni, Armin Leibundgut, Marc Glockshuber, Rudi Boehringer, Daniel Nat Commun Article Royal jelly (RJ) is produced by honeybees (Apis mellifera) as nutrition during larval development. The high viscosity of RJ originates from high concentrations of long lipoprotein filaments that include the glycosylated major royal jelly protein 1 (MRJP1), the small protein apisimin and insect lipids. Using cryo-electron microscopy we reveal the architecture and the composition of RJ filaments, in which the MRJP1 forms the outer shell of the assembly, surrounding stacked apisimin tetramers harbouring tightly packed lipids in the centre. The structural data rationalize the pH-dependent disassembly of RJ filaments in the gut of the larvae. Nature Publishing Group UK 2020-12-08 /pmc/articles/PMC7722742/ /pubmed/33293513 http://dx.doi.org/10.1038/s41467-020-20135-x Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Mattei, Simone
Ban, Arvid
Picenoni, Armin
Leibundgut, Marc
Glockshuber, Rudi
Boehringer, Daniel
Structure of native glycolipoprotein filaments in honeybee royal jelly
title Structure of native glycolipoprotein filaments in honeybee royal jelly
title_full Structure of native glycolipoprotein filaments in honeybee royal jelly
title_fullStr Structure of native glycolipoprotein filaments in honeybee royal jelly
title_full_unstemmed Structure of native glycolipoprotein filaments in honeybee royal jelly
title_short Structure of native glycolipoprotein filaments in honeybee royal jelly
title_sort structure of native glycolipoprotein filaments in honeybee royal jelly
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7722742/
https://www.ncbi.nlm.nih.gov/pubmed/33293513
http://dx.doi.org/10.1038/s41467-020-20135-x
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