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Influenza virus neuraminidase regulates host CD8(+) T-cell response in mice

Influenza A virus (IAV)-specific CD8(+) T-cell response was shown to provide protection against pandemic and seasonal influenza infections. However, the response was often relatively weak and the mechanism was unclear. Here, we show that the composition of IAV released from infected cells is regulat...

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Autores principales: Wu, Chung-Yi, Chuang, Hong-Yang, Wong, Chi-Huey
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7722854/
https://www.ncbi.nlm.nih.gov/pubmed/33293641
http://dx.doi.org/10.1038/s42003-020-01486-z
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author Wu, Chung-Yi
Chuang, Hong-Yang
Wong, Chi-Huey
author_facet Wu, Chung-Yi
Chuang, Hong-Yang
Wong, Chi-Huey
author_sort Wu, Chung-Yi
collection PubMed
description Influenza A virus (IAV)-specific CD8(+) T-cell response was shown to provide protection against pandemic and seasonal influenza infections. However, the response was often relatively weak and the mechanism was unclear. Here, we show that the composition of IAV released from infected cells is regulated by the neuraminidase (NA) activity and the cells infected by NA-defective virus cause intracellular viral protein accumulation and cell death. In addition, after uptake of NA-defective viruses by dendritic cells (DCs), an expression of the major histocompatibility complex class I is induced to activate IAV-specific CD8(+) T-cell response. When mice were infected by NA-defective IAV, a CD8(+) T-cell response to the highly conserved viral antigens including PB1, NP, HA, M1, M2 and NS1 was observed along with the increasing expression of IL10, IL12 and IL27. Vaccination of mice with NA-defective H1N1 A/WSN/33 induced a strong IAV-specific CD8(+) T cell response against H1N1, H3N2 and H5N1. This study reveals the role of NA in the IAV-specific CD8(+) T-cell response and virion assembly process, and provides an alternative direction toward the development of universal influenza vaccines.
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spelling pubmed-77228542020-12-11 Influenza virus neuraminidase regulates host CD8(+) T-cell response in mice Wu, Chung-Yi Chuang, Hong-Yang Wong, Chi-Huey Commun Biol Article Influenza A virus (IAV)-specific CD8(+) T-cell response was shown to provide protection against pandemic and seasonal influenza infections. However, the response was often relatively weak and the mechanism was unclear. Here, we show that the composition of IAV released from infected cells is regulated by the neuraminidase (NA) activity and the cells infected by NA-defective virus cause intracellular viral protein accumulation and cell death. In addition, after uptake of NA-defective viruses by dendritic cells (DCs), an expression of the major histocompatibility complex class I is induced to activate IAV-specific CD8(+) T-cell response. When mice were infected by NA-defective IAV, a CD8(+) T-cell response to the highly conserved viral antigens including PB1, NP, HA, M1, M2 and NS1 was observed along with the increasing expression of IL10, IL12 and IL27. Vaccination of mice with NA-defective H1N1 A/WSN/33 induced a strong IAV-specific CD8(+) T cell response against H1N1, H3N2 and H5N1. This study reveals the role of NA in the IAV-specific CD8(+) T-cell response and virion assembly process, and provides an alternative direction toward the development of universal influenza vaccines. Nature Publishing Group UK 2020-12-08 /pmc/articles/PMC7722854/ /pubmed/33293641 http://dx.doi.org/10.1038/s42003-020-01486-z Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Wu, Chung-Yi
Chuang, Hong-Yang
Wong, Chi-Huey
Influenza virus neuraminidase regulates host CD8(+) T-cell response in mice
title Influenza virus neuraminidase regulates host CD8(+) T-cell response in mice
title_full Influenza virus neuraminidase regulates host CD8(+) T-cell response in mice
title_fullStr Influenza virus neuraminidase regulates host CD8(+) T-cell response in mice
title_full_unstemmed Influenza virus neuraminidase regulates host CD8(+) T-cell response in mice
title_short Influenza virus neuraminidase regulates host CD8(+) T-cell response in mice
title_sort influenza virus neuraminidase regulates host cd8(+) t-cell response in mice
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7722854/
https://www.ncbi.nlm.nih.gov/pubmed/33293641
http://dx.doi.org/10.1038/s42003-020-01486-z
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