Cargando…

Long non-coding RNA SNHG9 inhibits ovarian cancer progression by sponging microRNA-214-5p

Ovarian cancer ranks 7th among the most common cancer types affecting women worldwide. A number of studies have confirmed that multiple long non-coding RNAs participate in the occurrence and progression of ovarian cancer. Small nucleolar RNA host gene 9 (SNHG9) serves a role in the progression of gl...

Descripción completa

Detalles Bibliográficos
Autores principales: Chen, Gao-Yang, Zhang, Zhi-Sheng, Chen, Yu, Li, Yan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7723070/
https://www.ncbi.nlm.nih.gov/pubmed/33363617
http://dx.doi.org/10.3892/ol.2020.12341
_version_ 1783620280648728576
author Chen, Gao-Yang
Zhang, Zhi-Sheng
Chen, Yu
Li, Yan
author_facet Chen, Gao-Yang
Zhang, Zhi-Sheng
Chen, Yu
Li, Yan
author_sort Chen, Gao-Yang
collection PubMed
description Ovarian cancer ranks 7th among the most common cancer types affecting women worldwide. A number of studies have confirmed that multiple long non-coding RNAs participate in the occurrence and progression of ovarian cancer. Small nucleolar RNA host gene 9 (SNHG9) serves a role in the progression of glioblastoma and pancreatic cancer. However, the specific biological function of SNHG9 in ovarian cancer has not yet been fully investigated. The present study aimed to determine the biological role and potential molecular mechanism underlying the influence of SNHG9 in ovarian cancer. SNHG9 expression in ovarian cancer cell lines and tissues were measured via reverse transcription-quantitative PCR analysis, and cell proliferation was detected via Cell Counting Kit-8 and colony formation assays. Flow cytometry was performed to assess cell cycle progression, and Transwell and wound healing assays were performed to assess cell invasion and migration abilities. Bioinformatics software was utilized to determine the target genes of SNHG9, which were subsequently verified via dual-luciferase reporter and RNA immunoprecipitation assays. The results demonstrated that SNHG9 expression was remarkably lower in ovarian cancer cell lines and tissues compared with the negative controls. Cell function assays demonstrated that decreased SNHG9 expression notably induced the migration, colony formation, proliferation and invasiveness of ovarian cancer cells. Furthermore, the inhibitory effect of SNHG9 on the migration, colony formation, proliferation and invasion of ovarian cancer cells was partially reversed by miR-214-5p upregulation. Thus, taken together, the current results suggest that SNHG9 may serve as a tumor suppressor gene in ovarian cancer by regulating the miR-214-5p/cryptochrome circadian regulator 2 axis.
format Online
Article
Text
id pubmed-7723070
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher D.A. Spandidos
record_format MEDLINE/PubMed
spelling pubmed-77230702020-12-23 Long non-coding RNA SNHG9 inhibits ovarian cancer progression by sponging microRNA-214-5p Chen, Gao-Yang Zhang, Zhi-Sheng Chen, Yu Li, Yan Oncol Lett Articles Ovarian cancer ranks 7th among the most common cancer types affecting women worldwide. A number of studies have confirmed that multiple long non-coding RNAs participate in the occurrence and progression of ovarian cancer. Small nucleolar RNA host gene 9 (SNHG9) serves a role in the progression of glioblastoma and pancreatic cancer. However, the specific biological function of SNHG9 in ovarian cancer has not yet been fully investigated. The present study aimed to determine the biological role and potential molecular mechanism underlying the influence of SNHG9 in ovarian cancer. SNHG9 expression in ovarian cancer cell lines and tissues were measured via reverse transcription-quantitative PCR analysis, and cell proliferation was detected via Cell Counting Kit-8 and colony formation assays. Flow cytometry was performed to assess cell cycle progression, and Transwell and wound healing assays were performed to assess cell invasion and migration abilities. Bioinformatics software was utilized to determine the target genes of SNHG9, which were subsequently verified via dual-luciferase reporter and RNA immunoprecipitation assays. The results demonstrated that SNHG9 expression was remarkably lower in ovarian cancer cell lines and tissues compared with the negative controls. Cell function assays demonstrated that decreased SNHG9 expression notably induced the migration, colony formation, proliferation and invasiveness of ovarian cancer cells. Furthermore, the inhibitory effect of SNHG9 on the migration, colony formation, proliferation and invasion of ovarian cancer cells was partially reversed by miR-214-5p upregulation. Thus, taken together, the current results suggest that SNHG9 may serve as a tumor suppressor gene in ovarian cancer by regulating the miR-214-5p/cryptochrome circadian regulator 2 axis. D.A. Spandidos 2021-02 2020-11-30 /pmc/articles/PMC7723070/ /pubmed/33363617 http://dx.doi.org/10.3892/ol.2020.12341 Text en Copyright: © Chen et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Chen, Gao-Yang
Zhang, Zhi-Sheng
Chen, Yu
Li, Yan
Long non-coding RNA SNHG9 inhibits ovarian cancer progression by sponging microRNA-214-5p
title Long non-coding RNA SNHG9 inhibits ovarian cancer progression by sponging microRNA-214-5p
title_full Long non-coding RNA SNHG9 inhibits ovarian cancer progression by sponging microRNA-214-5p
title_fullStr Long non-coding RNA SNHG9 inhibits ovarian cancer progression by sponging microRNA-214-5p
title_full_unstemmed Long non-coding RNA SNHG9 inhibits ovarian cancer progression by sponging microRNA-214-5p
title_short Long non-coding RNA SNHG9 inhibits ovarian cancer progression by sponging microRNA-214-5p
title_sort long non-coding rna snhg9 inhibits ovarian cancer progression by sponging microrna-214-5p
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7723070/
https://www.ncbi.nlm.nih.gov/pubmed/33363617
http://dx.doi.org/10.3892/ol.2020.12341
work_keys_str_mv AT chengaoyang longnoncodingrnasnhg9inhibitsovariancancerprogressionbyspongingmicrorna2145p
AT zhangzhisheng longnoncodingrnasnhg9inhibitsovariancancerprogressionbyspongingmicrorna2145p
AT chenyu longnoncodingrnasnhg9inhibitsovariancancerprogressionbyspongingmicrorna2145p
AT liyan longnoncodingrnasnhg9inhibitsovariancancerprogressionbyspongingmicrorna2145p