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Nuclear Waste and Biocatalysis: A Sustainable Liaison?
[Image: see text] It is well-known that energy-rich radiation induces water splitting, eventually yielding hydrogen peroxide. Synthetic applications, however, are scarce and to the best of our knowledge, the combination of radioactivity with enzyme-catalysis has not been considered yet. Peroxygenase...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Chemical
Society
2020
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7723303/ https://www.ncbi.nlm.nih.gov/pubmed/33312749 http://dx.doi.org/10.1021/acscatal.0c03059 |
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author | Zhang, Wuyuan Liu, Huanhuan van Schie, Morten M. C. H. Hagedoorn, Peter-Leon Alcalde, Miguel Denkova, Antonia G. Djanashvili, Kristina Hollmann, Frank |
author_facet | Zhang, Wuyuan Liu, Huanhuan van Schie, Morten M. C. H. Hagedoorn, Peter-Leon Alcalde, Miguel Denkova, Antonia G. Djanashvili, Kristina Hollmann, Frank |
author_sort | Zhang, Wuyuan |
collection | PubMed |
description | [Image: see text] It is well-known that energy-rich radiation induces water splitting, eventually yielding hydrogen peroxide. Synthetic applications, however, are scarce and to the best of our knowledge, the combination of radioactivity with enzyme-catalysis has not been considered yet. Peroxygenases utilize H(2)O(2) as an oxidant to promote highly selective oxyfunctionalization reactions but are also irreversibly inactivated in the presence of too high H(2)O(2) concentrations. Therefore, there is a need for efficient in situ H(2)O(2) generation methods. Here, we show that radiolytic water splitting can be used to promote specific biocatalytic oxyfunctionalization reactions. Parameters influencing the efficiency of the reaction and current limitations are shown. Particularly, oxidative inactivation of the biocatalyst by hydroxyl radicals influences the robustness of the overall reaction. Radical scavengers can alleviate this issue, but eventually, physical separation of the enzymes from the ionizing radiation will be necessary to achieve robust reaction schemes. We demonstrate that nuclear waste can also be used to drive selective, peroxygenase-catalyzed oxyfunctionalization reactions, challenging our view on nuclear waste in terms of sustainability. |
format | Online Article Text |
id | pubmed-7723303 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | American Chemical
Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-77233032020-12-09 Nuclear Waste and Biocatalysis: A Sustainable Liaison? Zhang, Wuyuan Liu, Huanhuan van Schie, Morten M. C. H. Hagedoorn, Peter-Leon Alcalde, Miguel Denkova, Antonia G. Djanashvili, Kristina Hollmann, Frank ACS Catal [Image: see text] It is well-known that energy-rich radiation induces water splitting, eventually yielding hydrogen peroxide. Synthetic applications, however, are scarce and to the best of our knowledge, the combination of radioactivity with enzyme-catalysis has not been considered yet. Peroxygenases utilize H(2)O(2) as an oxidant to promote highly selective oxyfunctionalization reactions but are also irreversibly inactivated in the presence of too high H(2)O(2) concentrations. Therefore, there is a need for efficient in situ H(2)O(2) generation methods. Here, we show that radiolytic water splitting can be used to promote specific biocatalytic oxyfunctionalization reactions. Parameters influencing the efficiency of the reaction and current limitations are shown. Particularly, oxidative inactivation of the biocatalyst by hydroxyl radicals influences the robustness of the overall reaction. Radical scavengers can alleviate this issue, but eventually, physical separation of the enzymes from the ionizing radiation will be necessary to achieve robust reaction schemes. We demonstrate that nuclear waste can also be used to drive selective, peroxygenase-catalyzed oxyfunctionalization reactions, challenging our view on nuclear waste in terms of sustainability. American Chemical Society 2020-11-20 2020-12-04 /pmc/articles/PMC7723303/ /pubmed/33312749 http://dx.doi.org/10.1021/acscatal.0c03059 Text en © 2020 American Chemical Society This is an open access article published under a Creative Commons Non-Commercial No Derivative Works (CC-BY-NC-ND) Attribution License (http://pubs.acs.org/page/policy/authorchoice_ccbyncnd_termsofuse.html) , which permits copying and redistribution of the article, and creation of adaptations, all for non-commercial purposes. |
spellingShingle | Zhang, Wuyuan Liu, Huanhuan van Schie, Morten M. C. H. Hagedoorn, Peter-Leon Alcalde, Miguel Denkova, Antonia G. Djanashvili, Kristina Hollmann, Frank Nuclear Waste and Biocatalysis: A Sustainable Liaison? |
title | Nuclear Waste
and Biocatalysis: A Sustainable Liaison? |
title_full | Nuclear Waste
and Biocatalysis: A Sustainable Liaison? |
title_fullStr | Nuclear Waste
and Biocatalysis: A Sustainable Liaison? |
title_full_unstemmed | Nuclear Waste
and Biocatalysis: A Sustainable Liaison? |
title_short | Nuclear Waste
and Biocatalysis: A Sustainable Liaison? |
title_sort | nuclear waste
and biocatalysis: a sustainable liaison? |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7723303/ https://www.ncbi.nlm.nih.gov/pubmed/33312749 http://dx.doi.org/10.1021/acscatal.0c03059 |
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