Integration of Cell-Free Expression and Solid-State NMR to Investigate the Dynamic Properties of Different Sites of the Growth Hormone Secretagogue Receptor

Cell-free expression represents an attractive method to produce large quantities of selectively labeled protein for NMR applications. Here, cell-free expression was used to label specific regions of the growth hormone secretagogue receptor (GHSR) with NMR-active isotopes. The GHSR is a member of the...

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Detalles Bibliográficos
Autores principales: Pacull, Emelyne M., Sendker, Franziska, Bernhard, Frank, Scheidt, Holger A., Schmidt, Peter, Huster, Daniel, Krug, Ulrike
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Pharmacology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7723455/
https://www.ncbi.nlm.nih.gov/pubmed/33324203
http://dx.doi.org/10.3389/fphar.2020.562113

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