A dual-deaminase CRISPR base editor enables concurrent adenine and cytosine editing

Existing adenine and cytosine base editors induce only a single type of modification, limiting the range of DNA alterations that can be created. Here we describe a CRISPR-Cas9-based synchronous programmable adenine and cytosine editor (SPACE) that can concurrently introduce A-to-G and C-to-T substit...

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Autores principales: Grünewald, Julian, Zhou, Ronghao, Lareau, Caleb A., Garcia, Sara P., Iyer, Sowmya, Miller, Bret R., Langner, Lukas M., Hsu, Jonathan Y., Aryee, Martin J., Joung, J. Keith
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7723518/
https://www.ncbi.nlm.nih.gov/pubmed/32483364
http://dx.doi.org/10.1038/s41587-020-0535-y
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author Grünewald, Julian
Zhou, Ronghao
Lareau, Caleb A.
Garcia, Sara P.
Iyer, Sowmya
Miller, Bret R.
Langner, Lukas M.
Hsu, Jonathan Y.
Aryee, Martin J.
Joung, J. Keith
author_facet Grünewald, Julian
Zhou, Ronghao
Lareau, Caleb A.
Garcia, Sara P.
Iyer, Sowmya
Miller, Bret R.
Langner, Lukas M.
Hsu, Jonathan Y.
Aryee, Martin J.
Joung, J. Keith
author_sort Grünewald, Julian
collection PubMed
description Existing adenine and cytosine base editors induce only a single type of modification, limiting the range of DNA alterations that can be created. Here we describe a CRISPR-Cas9-based synchronous programmable adenine and cytosine editor (SPACE) that can concurrently introduce A-to-G and C-to-T substitutions with minimal RNA off-target edits. SPACE expands the range of possible DNA sequence alterations, broadening the research applications of CRISPR base editors.
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spelling pubmed-77235182020-12-08 A dual-deaminase CRISPR base editor enables concurrent adenine and cytosine editing Grünewald, Julian Zhou, Ronghao Lareau, Caleb A. Garcia, Sara P. Iyer, Sowmya Miller, Bret R. Langner, Lukas M. Hsu, Jonathan Y. Aryee, Martin J. Joung, J. Keith Nat Biotechnol Article Existing adenine and cytosine base editors induce only a single type of modification, limiting the range of DNA alterations that can be created. Here we describe a CRISPR-Cas9-based synchronous programmable adenine and cytosine editor (SPACE) that can concurrently introduce A-to-G and C-to-T substitutions with minimal RNA off-target edits. SPACE expands the range of possible DNA sequence alterations, broadening the research applications of CRISPR base editors. 2020-06-01 2020-07 /pmc/articles/PMC7723518/ /pubmed/32483364 http://dx.doi.org/10.1038/s41587-020-0535-y Text en Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Grünewald, Julian
Zhou, Ronghao
Lareau, Caleb A.
Garcia, Sara P.
Iyer, Sowmya
Miller, Bret R.
Langner, Lukas M.
Hsu, Jonathan Y.
Aryee, Martin J.
Joung, J. Keith
A dual-deaminase CRISPR base editor enables concurrent adenine and cytosine editing
title A dual-deaminase CRISPR base editor enables concurrent adenine and cytosine editing
title_full A dual-deaminase CRISPR base editor enables concurrent adenine and cytosine editing
title_fullStr A dual-deaminase CRISPR base editor enables concurrent adenine and cytosine editing
title_full_unstemmed A dual-deaminase CRISPR base editor enables concurrent adenine and cytosine editing
title_short A dual-deaminase CRISPR base editor enables concurrent adenine and cytosine editing
title_sort dual-deaminase crispr base editor enables concurrent adenine and cytosine editing
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7723518/
https://www.ncbi.nlm.nih.gov/pubmed/32483364
http://dx.doi.org/10.1038/s41587-020-0535-y
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