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Structure–Function Relationships of Healthy and Osteoarthritic Human Tibial Cartilage: Experimental and Numerical Investigation

Relationships between composition, structure and constituent-specific functional properties of human articular cartilage at different stages of osteoarthritis (OA) are poorly known. We established these relationships by comparison of elastic, viscoelastic and fibril-reinforced poroelastic mechanical...

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Autores principales: Ebrahimi, Mohammadhossein, Turunen, Mikael J., Finnilä, Mikko A., Joukainen, Antti, Kröger, Heikki, Saarakkala, Simo, Korhonen, Rami K., Tanska, Petri
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer International Publishing 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7723942/
https://www.ncbi.nlm.nih.gov/pubmed/32648191
http://dx.doi.org/10.1007/s10439-020-02559-0
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author Ebrahimi, Mohammadhossein
Turunen, Mikael J.
Finnilä, Mikko A.
Joukainen, Antti
Kröger, Heikki
Saarakkala, Simo
Korhonen, Rami K.
Tanska, Petri
author_facet Ebrahimi, Mohammadhossein
Turunen, Mikael J.
Finnilä, Mikko A.
Joukainen, Antti
Kröger, Heikki
Saarakkala, Simo
Korhonen, Rami K.
Tanska, Petri
author_sort Ebrahimi, Mohammadhossein
collection PubMed
description Relationships between composition, structure and constituent-specific functional properties of human articular cartilage at different stages of osteoarthritis (OA) are poorly known. We established these relationships by comparison of elastic, viscoelastic and fibril-reinforced poroelastic mechanical properties with microscopic and spectroscopic analysis of structure and composition of healthy and osteoarthritic human tibial cartilage (n = 27). At a low frequency (0.005 Hz), proteoglycan content correlated negatively and collagen content correlated positively with the phase difference (i.e. tissue viscosity). At a high-frequency regime (> 0.05 Hz), proteoglycan content correlated negatively and collagen orientation angle correlated positively with the phase difference. Proteoglycans were lost in the early and advanced OA groups compared to the healthy group, while the superficial collagen orientation angle was greater only in the advanced OA group compared to the healthy group. Simultaneously, the initial fibril network modulus (fibril pretension) was smaller in the early and advanced OA groups compared to the healthy group. These findings suggest different mechanisms contribute to cartilage viscosity in low and high frequencies, and that the loss of superficial collagen pretension during early OA is due to lower tissue swelling (PG loss), while in advanced OA, both collagen disorganization and lower swelling modulate the collagen fibril pretension. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s10439-020-02559-0) contains supplementary material, which is available to authorized users.
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spelling pubmed-77239422020-12-14 Structure–Function Relationships of Healthy and Osteoarthritic Human Tibial Cartilage: Experimental and Numerical Investigation Ebrahimi, Mohammadhossein Turunen, Mikael J. Finnilä, Mikko A. Joukainen, Antti Kröger, Heikki Saarakkala, Simo Korhonen, Rami K. Tanska, Petri Ann Biomed Eng Original Article Relationships between composition, structure and constituent-specific functional properties of human articular cartilage at different stages of osteoarthritis (OA) are poorly known. We established these relationships by comparison of elastic, viscoelastic and fibril-reinforced poroelastic mechanical properties with microscopic and spectroscopic analysis of structure and composition of healthy and osteoarthritic human tibial cartilage (n = 27). At a low frequency (0.005 Hz), proteoglycan content correlated negatively and collagen content correlated positively with the phase difference (i.e. tissue viscosity). At a high-frequency regime (> 0.05 Hz), proteoglycan content correlated negatively and collagen orientation angle correlated positively with the phase difference. Proteoglycans were lost in the early and advanced OA groups compared to the healthy group, while the superficial collagen orientation angle was greater only in the advanced OA group compared to the healthy group. Simultaneously, the initial fibril network modulus (fibril pretension) was smaller in the early and advanced OA groups compared to the healthy group. These findings suggest different mechanisms contribute to cartilage viscosity in low and high frequencies, and that the loss of superficial collagen pretension during early OA is due to lower tissue swelling (PG loss), while in advanced OA, both collagen disorganization and lower swelling modulate the collagen fibril pretension. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s10439-020-02559-0) contains supplementary material, which is available to authorized users. Springer International Publishing 2020-07-09 2020 /pmc/articles/PMC7723942/ /pubmed/32648191 http://dx.doi.org/10.1007/s10439-020-02559-0 Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Original Article
Ebrahimi, Mohammadhossein
Turunen, Mikael J.
Finnilä, Mikko A.
Joukainen, Antti
Kröger, Heikki
Saarakkala, Simo
Korhonen, Rami K.
Tanska, Petri
Structure–Function Relationships of Healthy and Osteoarthritic Human Tibial Cartilage: Experimental and Numerical Investigation
title Structure–Function Relationships of Healthy and Osteoarthritic Human Tibial Cartilage: Experimental and Numerical Investigation
title_full Structure–Function Relationships of Healthy and Osteoarthritic Human Tibial Cartilage: Experimental and Numerical Investigation
title_fullStr Structure–Function Relationships of Healthy and Osteoarthritic Human Tibial Cartilage: Experimental and Numerical Investigation
title_full_unstemmed Structure–Function Relationships of Healthy and Osteoarthritic Human Tibial Cartilage: Experimental and Numerical Investigation
title_short Structure–Function Relationships of Healthy and Osteoarthritic Human Tibial Cartilage: Experimental and Numerical Investigation
title_sort structure–function relationships of healthy and osteoarthritic human tibial cartilage: experimental and numerical investigation
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7723942/
https://www.ncbi.nlm.nih.gov/pubmed/32648191
http://dx.doi.org/10.1007/s10439-020-02559-0
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