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The Bone-Forming Properties of Periosteum-Derived Cells Differ Between Harvest Sites

The development of alternatives for autologous bone grafts is a major focus of bone tissue engineering. To produce living bone-forming implants, skeletal stem and progenitor cells (SSPCs) are envisioned as key ingredients. SSPCs can be obtained from different tissues including bone marrow, adipose t...

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Autores principales: Groeneveldt, Lisanne C., Herpelinck, Tim, Maréchal, Marina, Politis, Constantinus, van IJcken, Wilfred F. J., Huylebroeck, Danny, Geris, Liesbet, Mulugeta, Eskeatnaf, Luyten, Frank P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7723972/
https://www.ncbi.nlm.nih.gov/pubmed/33324630
http://dx.doi.org/10.3389/fcell.2020.554984
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author Groeneveldt, Lisanne C.
Herpelinck, Tim
Maréchal, Marina
Politis, Constantinus
van IJcken, Wilfred F. J.
Huylebroeck, Danny
Geris, Liesbet
Mulugeta, Eskeatnaf
Luyten, Frank P.
author_facet Groeneveldt, Lisanne C.
Herpelinck, Tim
Maréchal, Marina
Politis, Constantinus
van IJcken, Wilfred F. J.
Huylebroeck, Danny
Geris, Liesbet
Mulugeta, Eskeatnaf
Luyten, Frank P.
author_sort Groeneveldt, Lisanne C.
collection PubMed
description The development of alternatives for autologous bone grafts is a major focus of bone tissue engineering. To produce living bone-forming implants, skeletal stem and progenitor cells (SSPCs) are envisioned as key ingredients. SSPCs can be obtained from different tissues including bone marrow, adipose tissue, dental pulp, and periosteum. Human periosteum-derived cells (hPDCs) exhibit progenitor cell characteristics and have well-documented in vivo bone formation potency. Here, we have characterized and compared hPDCs derived from tibia with craniofacial hPDCs, from maxilla and mandible, respectively, each representing a potential source for cell-based tissue engineered implants for craniofacial applications. Maxilla and mandible-derived hPDCs display similar growth curves as tibial hPDCs, with equal trilineage differentiation potential toward chondrogenic, osteogenic, and adipogenic cells. These craniofacial hPDCs are positive for SSPC-markers CD73, CD164, and Podoplanin (PDPN), and negative for CD146, hematopoietic and endothelial lineage markers. Bulk RNA-sequencing identified genes that are differentially expressed between the three sources of hPDC. In particular, differential expression was found for genes of the HOX and DLX family, for SOX9 and genes involved in skeletal system development. The in vivo bone formation, 8 weeks after ectopic implantation in nude mice, was observed in constructs seeded with tibial and mandibular hPDCs. Taken together, we provide evidence that hPDCs show different profiles and properties according to their anatomical origin, and that craniofacial hPDCs are potential sources for cell-based bone tissue engineering strategies. The mandible-derived hPDCs display - both in vitro and in vivo - chondrogenic and osteogenic differentiation potential, which supports their future testing for use in craniofacial bone regeneration applications.
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spelling pubmed-77239722020-12-14 The Bone-Forming Properties of Periosteum-Derived Cells Differ Between Harvest Sites Groeneveldt, Lisanne C. Herpelinck, Tim Maréchal, Marina Politis, Constantinus van IJcken, Wilfred F. J. Huylebroeck, Danny Geris, Liesbet Mulugeta, Eskeatnaf Luyten, Frank P. Front Cell Dev Biol Cell and Developmental Biology The development of alternatives for autologous bone grafts is a major focus of bone tissue engineering. To produce living bone-forming implants, skeletal stem and progenitor cells (SSPCs) are envisioned as key ingredients. SSPCs can be obtained from different tissues including bone marrow, adipose tissue, dental pulp, and periosteum. Human periosteum-derived cells (hPDCs) exhibit progenitor cell characteristics and have well-documented in vivo bone formation potency. Here, we have characterized and compared hPDCs derived from tibia with craniofacial hPDCs, from maxilla and mandible, respectively, each representing a potential source for cell-based tissue engineered implants for craniofacial applications. Maxilla and mandible-derived hPDCs display similar growth curves as tibial hPDCs, with equal trilineage differentiation potential toward chondrogenic, osteogenic, and adipogenic cells. These craniofacial hPDCs are positive for SSPC-markers CD73, CD164, and Podoplanin (PDPN), and negative for CD146, hematopoietic and endothelial lineage markers. Bulk RNA-sequencing identified genes that are differentially expressed between the three sources of hPDC. In particular, differential expression was found for genes of the HOX and DLX family, for SOX9 and genes involved in skeletal system development. The in vivo bone formation, 8 weeks after ectopic implantation in nude mice, was observed in constructs seeded with tibial and mandibular hPDCs. Taken together, we provide evidence that hPDCs show different profiles and properties according to their anatomical origin, and that craniofacial hPDCs are potential sources for cell-based bone tissue engineering strategies. The mandible-derived hPDCs display - both in vitro and in vivo - chondrogenic and osteogenic differentiation potential, which supports their future testing for use in craniofacial bone regeneration applications. Frontiers Media S.A. 2020-11-25 /pmc/articles/PMC7723972/ /pubmed/33324630 http://dx.doi.org/10.3389/fcell.2020.554984 Text en Copyright © 2020 Groeneveldt, Herpelinck, Maréchal, Politis, van IJcken, Huylebroeck, Geris, Mulugeta and Luyten. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cell and Developmental Biology
Groeneveldt, Lisanne C.
Herpelinck, Tim
Maréchal, Marina
Politis, Constantinus
van IJcken, Wilfred F. J.
Huylebroeck, Danny
Geris, Liesbet
Mulugeta, Eskeatnaf
Luyten, Frank P.
The Bone-Forming Properties of Periosteum-Derived Cells Differ Between Harvest Sites
title The Bone-Forming Properties of Periosteum-Derived Cells Differ Between Harvest Sites
title_full The Bone-Forming Properties of Periosteum-Derived Cells Differ Between Harvest Sites
title_fullStr The Bone-Forming Properties of Periosteum-Derived Cells Differ Between Harvest Sites
title_full_unstemmed The Bone-Forming Properties of Periosteum-Derived Cells Differ Between Harvest Sites
title_short The Bone-Forming Properties of Periosteum-Derived Cells Differ Between Harvest Sites
title_sort bone-forming properties of periosteum-derived cells differ between harvest sites
topic Cell and Developmental Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7723972/
https://www.ncbi.nlm.nih.gov/pubmed/33324630
http://dx.doi.org/10.3389/fcell.2020.554984
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