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Enhancing the Heterologous Fructosyltransferase Activity of Kluyveromyces lactis: Developing a Scaled-Up Process and Abolishing Invertase by CRISPR/Cas9 Genome Editing

The enzymatic production of prebiotic fructo-oligosaccharides (FOS) from sucrose involves fructosyltransferases (FFTs) and invertases, both of which catalyze forward (transferase) and reverse (hydrolysis) reactions. FOS yields can therefore be increased by favoring the forward reaction. We investiga...

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Detalles Bibliográficos
Autores principales:	Burghardt, Jan Philipp, Fan, Rong, Baas, Markus, Eckhardt, Dustin, Gerlach, Doreen, Czermak, Peter
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Frontiers Media S.A. 2020
Materias:	Bioengineering and Biotechnology
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7724039/ https://www.ncbi.nlm.nih.gov/pubmed/33324627 http://dx.doi.org/10.3389/fbioe.2020.607507

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