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Long intergenic noncoding RNA smad7 (Linc‐smad7) promotes the epithelial‐mesenchymal transition of HCC by targeting the miR‑125b/SIRT6 axis

Long intergenic noncoding RNA smad7 (Linc‐smad7) has been recently identified as a new long non‐coding RNA (lncRNA). However, the role of Linc‐smad7 in the tumourigenesis of human cancers remains unknown. This study uncovered that Linc‐smad7 was increased in HCC samples and HCC cell lines using RT‐q...

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Detalles Bibliográficos
Autores principales: Han, Lili, Jia, Lijun, Zan, Ying
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7724296/
https://www.ncbi.nlm.nih.gov/pubmed/33037850
http://dx.doi.org/10.1002/cam4.3515
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author Han, Lili
Jia, Lijun
Zan, Ying
author_facet Han, Lili
Jia, Lijun
Zan, Ying
author_sort Han, Lili
collection PubMed
description Long intergenic noncoding RNA smad7 (Linc‐smad7) has been recently identified as a new long non‐coding RNA (lncRNA). However, the role of Linc‐smad7 in the tumourigenesis of human cancers remains unknown. This study uncovered that Linc‐smad7 was increased in HCC samples and HCC cell lines using RT‐qPCR assays. Furthermore, the overexpression of Linc‐smad7 indicated poor clinicopathological features and outcomes for HCC patients. In addition, Linc‐smad7 promoted HCC cells proliferation, migration, invasion and EMT, as determined by MTT, colony formation, Transwell assays and western blot analysis. Functionally, it was demonstrated that Linc‐smad7 could bind with microRNA‑125b (miR‑125b), and the restoration of miR‑125b rescued the promoting effects of Linc‐smad7 on HCC cells. Finally, it was observed that sirtuin 6 (SIRT6) was positively regulated by Linc‐smad7 in HCC as the direct target of miR‑125b, and decreased SIRT6 reversed the effects of Linc‐smad7 on promoting HCC. In conclusion, the current study first identified Linc‐smad7 is increased in HCC, facilitating HCC cells proliferation, migration, invasion and EMT via regulating the miR‑125b/SIRT6 axis.
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spelling pubmed-77242962020-12-13 Long intergenic noncoding RNA smad7 (Linc‐smad7) promotes the epithelial‐mesenchymal transition of HCC by targeting the miR‑125b/SIRT6 axis Han, Lili Jia, Lijun Zan, Ying Cancer Med Cancer Biology Long intergenic noncoding RNA smad7 (Linc‐smad7) has been recently identified as a new long non‐coding RNA (lncRNA). However, the role of Linc‐smad7 in the tumourigenesis of human cancers remains unknown. This study uncovered that Linc‐smad7 was increased in HCC samples and HCC cell lines using RT‐qPCR assays. Furthermore, the overexpression of Linc‐smad7 indicated poor clinicopathological features and outcomes for HCC patients. In addition, Linc‐smad7 promoted HCC cells proliferation, migration, invasion and EMT, as determined by MTT, colony formation, Transwell assays and western blot analysis. Functionally, it was demonstrated that Linc‐smad7 could bind with microRNA‑125b (miR‑125b), and the restoration of miR‑125b rescued the promoting effects of Linc‐smad7 on HCC cells. Finally, it was observed that sirtuin 6 (SIRT6) was positively regulated by Linc‐smad7 in HCC as the direct target of miR‑125b, and decreased SIRT6 reversed the effects of Linc‐smad7 on promoting HCC. In conclusion, the current study first identified Linc‐smad7 is increased in HCC, facilitating HCC cells proliferation, migration, invasion and EMT via regulating the miR‑125b/SIRT6 axis. John Wiley and Sons Inc. 2020-10-10 /pmc/articles/PMC7724296/ /pubmed/33037850 http://dx.doi.org/10.1002/cam4.3515 Text en © 2020 The Authors. Cancer Medicine published by John Wiley & Sons Ltd. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Cancer Biology
Han, Lili
Jia, Lijun
Zan, Ying
Long intergenic noncoding RNA smad7 (Linc‐smad7) promotes the epithelial‐mesenchymal transition of HCC by targeting the miR‑125b/SIRT6 axis
title Long intergenic noncoding RNA smad7 (Linc‐smad7) promotes the epithelial‐mesenchymal transition of HCC by targeting the miR‑125b/SIRT6 axis
title_full Long intergenic noncoding RNA smad7 (Linc‐smad7) promotes the epithelial‐mesenchymal transition of HCC by targeting the miR‑125b/SIRT6 axis
title_fullStr Long intergenic noncoding RNA smad7 (Linc‐smad7) promotes the epithelial‐mesenchymal transition of HCC by targeting the miR‑125b/SIRT6 axis
title_full_unstemmed Long intergenic noncoding RNA smad7 (Linc‐smad7) promotes the epithelial‐mesenchymal transition of HCC by targeting the miR‑125b/SIRT6 axis
title_short Long intergenic noncoding RNA smad7 (Linc‐smad7) promotes the epithelial‐mesenchymal transition of HCC by targeting the miR‑125b/SIRT6 axis
title_sort long intergenic noncoding rna smad7 (linc‐smad7) promotes the epithelial‐mesenchymal transition of hcc by targeting the mir‑125b/sirt6 axis
topic Cancer Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7724296/
https://www.ncbi.nlm.nih.gov/pubmed/33037850
http://dx.doi.org/10.1002/cam4.3515
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