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Effect of Low Intensity Magnetic Field Stimulation on Calcium-Mediated Cytotoxicity After Mild Spinal Cord Contusion Injury in Rats
BACKGROUND: Magnetic field (MF) stimulation has the potential to reduce secondary damage and promote functional recovery after neural tissue injury. The study aimed to observe the effect of very low intensity (17.96µT) MF on general body condition, secondary damage, pain status, and locomotion. METH...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
SAGE Publications
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7724432/ https://www.ncbi.nlm.nih.gov/pubmed/33335356 http://dx.doi.org/10.1177/0972753120950072 |
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author | Bhattacharyya, Supti Sahu, Shivani Kaur, Sajeev Jain, Suman |
author_facet | Bhattacharyya, Supti Sahu, Shivani Kaur, Sajeev Jain, Suman |
author_sort | Bhattacharyya, Supti |
collection | PubMed |
description | BACKGROUND: Magnetic field (MF) stimulation has the potential to reduce secondary damage and promote functional recovery after neural tissue injury. The study aimed to observe the effect of very low intensity (17.96µT) MF on general body condition, secondary damage, pain status, and locomotion. METHODS: We exposed rats to MF (2 h/day × 3 weeks) after 6.25 mm contusion spinal injury. Locomotor behavior was evaluated by BBB score, pain assessment was done by recording threshold for tail flick, expression of voltage-gated calcium channels and extent of secondary damage in the spinal cord was assessed by immunofluorescence and Cresyl violet staining, respectively. RESULTS: A significant (p ≤ .001) improvement in bladder function as well as BBB score was observed after MF exposure in comparison with sham and SCI over the observation period of 3 weeks. SCI group showed an increase in the threshold for vocalization after discharge, which decreased following MF exposure. Cresyl violet staining showed significantly higher tissue sparing (73%) at the epicenter after MF exposure when compared to SCI group. This was accompanied with a significant decrease in calcium channel expression in MF group as compared to SCI. CONCLUSION: The results suggest facilitation of sensory-motor recovery after MF exposure, which could be due to attenuation of secondary damage and calcium-mediated excitotoxicity in a mild contusion rat model of SCI. |
format | Online Article Text |
id | pubmed-7724432 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | SAGE Publications |
record_format | MEDLINE/PubMed |
spelling | pubmed-77244322020-12-16 Effect of Low Intensity Magnetic Field Stimulation on Calcium-Mediated Cytotoxicity After Mild Spinal Cord Contusion Injury in Rats Bhattacharyya, Supti Sahu, Shivani Kaur, Sajeev Jain, Suman Ann Neurosci Original Article BACKGROUND: Magnetic field (MF) stimulation has the potential to reduce secondary damage and promote functional recovery after neural tissue injury. The study aimed to observe the effect of very low intensity (17.96µT) MF on general body condition, secondary damage, pain status, and locomotion. METHODS: We exposed rats to MF (2 h/day × 3 weeks) after 6.25 mm contusion spinal injury. Locomotor behavior was evaluated by BBB score, pain assessment was done by recording threshold for tail flick, expression of voltage-gated calcium channels and extent of secondary damage in the spinal cord was assessed by immunofluorescence and Cresyl violet staining, respectively. RESULTS: A significant (p ≤ .001) improvement in bladder function as well as BBB score was observed after MF exposure in comparison with sham and SCI over the observation period of 3 weeks. SCI group showed an increase in the threshold for vocalization after discharge, which decreased following MF exposure. Cresyl violet staining showed significantly higher tissue sparing (73%) at the epicenter after MF exposure when compared to SCI group. This was accompanied with a significant decrease in calcium channel expression in MF group as compared to SCI. CONCLUSION: The results suggest facilitation of sensory-motor recovery after MF exposure, which could be due to attenuation of secondary damage and calcium-mediated excitotoxicity in a mild contusion rat model of SCI. SAGE Publications 2020-10-07 2020-04 /pmc/articles/PMC7724432/ /pubmed/33335356 http://dx.doi.org/10.1177/0972753120950072 Text en © 2020 Indian Academy of Neurosciences (IAN) https://creativecommons.org/licenses/by-nc/4.0/ This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access page (https://us.sagepub.com/en-us/nam/open-access-at-sage). |
spellingShingle | Original Article Bhattacharyya, Supti Sahu, Shivani Kaur, Sajeev Jain, Suman Effect of Low Intensity Magnetic Field Stimulation on Calcium-Mediated Cytotoxicity After Mild Spinal Cord Contusion Injury in Rats |
title | Effect of Low Intensity Magnetic Field Stimulation on Calcium-Mediated
Cytotoxicity After Mild Spinal Cord Contusion Injury in Rats |
title_full | Effect of Low Intensity Magnetic Field Stimulation on Calcium-Mediated
Cytotoxicity After Mild Spinal Cord Contusion Injury in Rats |
title_fullStr | Effect of Low Intensity Magnetic Field Stimulation on Calcium-Mediated
Cytotoxicity After Mild Spinal Cord Contusion Injury in Rats |
title_full_unstemmed | Effect of Low Intensity Magnetic Field Stimulation on Calcium-Mediated
Cytotoxicity After Mild Spinal Cord Contusion Injury in Rats |
title_short | Effect of Low Intensity Magnetic Field Stimulation on Calcium-Mediated
Cytotoxicity After Mild Spinal Cord Contusion Injury in Rats |
title_sort | effect of low intensity magnetic field stimulation on calcium-mediated
cytotoxicity after mild spinal cord contusion injury in rats |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7724432/ https://www.ncbi.nlm.nih.gov/pubmed/33335356 http://dx.doi.org/10.1177/0972753120950072 |
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