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Regulatory effects of chicken TRIM25 on the replication of ALV-A and the MDA5-mediated type I interferon response
This study focuses on the immunoregulatory effects of chicken TRIM25 on the replication of subgroup A of avian leukosis virus (ALV-A) and the MDA5-mediated type I interferon response. The ALV-A-SDAU09C1 strain was inoculated into DF1 cells and 1-day-old SPF chickens, and the expression of TRIM25 was...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7724733/ https://www.ncbi.nlm.nih.gov/pubmed/33298177 http://dx.doi.org/10.1186/s13567-020-00870-1 |
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author | Zhou, Jin-run Liu, Jun-hong Li, Hong-mei Zhao, Yue Cheng, Ziqiang Hou, Yan-meng Guo, Hui-jun |
author_facet | Zhou, Jin-run Liu, Jun-hong Li, Hong-mei Zhao, Yue Cheng, Ziqiang Hou, Yan-meng Guo, Hui-jun |
author_sort | Zhou, Jin-run |
collection | PubMed |
description | This study focuses on the immunoregulatory effects of chicken TRIM25 on the replication of subgroup A of avian leukosis virus (ALV-A) and the MDA5-mediated type I interferon response. The ALV-A-SDAU09C1 strain was inoculated into DF1 cells and 1-day-old SPF chickens, and the expression of TRIM25 was detected at different time points after inoculation. A recombinant overexpression plasmid containing the chicken TRIM25 gene (TRIM25-GFP) was constructed and transfected into DF1 cells to analyse the effects of the overexpression of chicken TRIM25 on the replication of ALV-A and the expression of MDA5, MAVS and IFN-β. A small interfering RNA targeting chicken TRIM25 (TRIM25-siRNA) was prepared and transfected into DF1 cells to assess the effects of the knockdown of chicken TRIM25 on the replication of ALV-A and the expression of MDA5, MAVS and IFN-β. The results showed that chicken TRIM25 was significantly upregulated at all time points both in ALV-A-infected cells and in ALV-A-infected chickens. Overexpression of chicken TRIM25 in DF1 cells dramatically decreased the antigenic titres of ALV-A in the cell supernatant and upregulated the relative expression of MDA5, MAVS and IFN-β induced by ALV-A or by poly(I:C); in contrast, knockdown of chicken TRIM25 significantly increased the antigenic titres of ALV-A and downregulated the relative expression of MDA5, MAVS and IFN-β. It can be concluded that chicken TRIM25 can inhibit the replication of ALV-A and upregulate the MDA5 receptor-mediated type I interferon response in chickens. This study can help improve the understanding of the antiviral activities of chicken TRIM25 and enrich the knowledge of antiviral responses in chickens. |
format | Online Article Text |
id | pubmed-7724733 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-77247332020-12-09 Regulatory effects of chicken TRIM25 on the replication of ALV-A and the MDA5-mediated type I interferon response Zhou, Jin-run Liu, Jun-hong Li, Hong-mei Zhao, Yue Cheng, Ziqiang Hou, Yan-meng Guo, Hui-jun Vet Res Research Article This study focuses on the immunoregulatory effects of chicken TRIM25 on the replication of subgroup A of avian leukosis virus (ALV-A) and the MDA5-mediated type I interferon response. The ALV-A-SDAU09C1 strain was inoculated into DF1 cells and 1-day-old SPF chickens, and the expression of TRIM25 was detected at different time points after inoculation. A recombinant overexpression plasmid containing the chicken TRIM25 gene (TRIM25-GFP) was constructed and transfected into DF1 cells to analyse the effects of the overexpression of chicken TRIM25 on the replication of ALV-A and the expression of MDA5, MAVS and IFN-β. A small interfering RNA targeting chicken TRIM25 (TRIM25-siRNA) was prepared and transfected into DF1 cells to assess the effects of the knockdown of chicken TRIM25 on the replication of ALV-A and the expression of MDA5, MAVS and IFN-β. The results showed that chicken TRIM25 was significantly upregulated at all time points both in ALV-A-infected cells and in ALV-A-infected chickens. Overexpression of chicken TRIM25 in DF1 cells dramatically decreased the antigenic titres of ALV-A in the cell supernatant and upregulated the relative expression of MDA5, MAVS and IFN-β induced by ALV-A or by poly(I:C); in contrast, knockdown of chicken TRIM25 significantly increased the antigenic titres of ALV-A and downregulated the relative expression of MDA5, MAVS and IFN-β. It can be concluded that chicken TRIM25 can inhibit the replication of ALV-A and upregulate the MDA5 receptor-mediated type I interferon response in chickens. This study can help improve the understanding of the antiviral activities of chicken TRIM25 and enrich the knowledge of antiviral responses in chickens. BioMed Central 2020-12-09 2020 /pmc/articles/PMC7724733/ /pubmed/33298177 http://dx.doi.org/10.1186/s13567-020-00870-1 Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Article Zhou, Jin-run Liu, Jun-hong Li, Hong-mei Zhao, Yue Cheng, Ziqiang Hou, Yan-meng Guo, Hui-jun Regulatory effects of chicken TRIM25 on the replication of ALV-A and the MDA5-mediated type I interferon response |
title | Regulatory effects of chicken TRIM25 on the replication of ALV-A and the MDA5-mediated type I interferon response |
title_full | Regulatory effects of chicken TRIM25 on the replication of ALV-A and the MDA5-mediated type I interferon response |
title_fullStr | Regulatory effects of chicken TRIM25 on the replication of ALV-A and the MDA5-mediated type I interferon response |
title_full_unstemmed | Regulatory effects of chicken TRIM25 on the replication of ALV-A and the MDA5-mediated type I interferon response |
title_short | Regulatory effects of chicken TRIM25 on the replication of ALV-A and the MDA5-mediated type I interferon response |
title_sort | regulatory effects of chicken trim25 on the replication of alv-a and the mda5-mediated type i interferon response |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7724733/ https://www.ncbi.nlm.nih.gov/pubmed/33298177 http://dx.doi.org/10.1186/s13567-020-00870-1 |
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