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Distribution, treatment outcome and genetic diversity of Leishmania species in military personnel from Colombia with cutaneous leishmaniasis

BACKGROUND: Leishmaniasis is one of the most important infectious diseases affecting the Colombian National Army due to the high number of reported cases and exposure throughout military operations in endemic areas. The main aim of this study was to estimate the geographical distribution along with...

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Autores principales: Correa-Cárdenas, Camilo A., Pérez, Julie, Patino, Luz H., Ramírez, Juan David, Duque, Maria Clara, Romero, Yanira, Cantillo-Barraza, Omar, Rodríguez, Omaira, Alvarado, Maria Teresa, Cruz, Claudia, Méndez, Claudia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2020
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7724885/
https://www.ncbi.nlm.nih.gov/pubmed/33297972
http://dx.doi.org/10.1186/s12879-020-05529-y
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author Correa-Cárdenas, Camilo A.
Pérez, Julie
Patino, Luz H.
Ramírez, Juan David
Duque, Maria Clara
Romero, Yanira
Cantillo-Barraza, Omar
Rodríguez, Omaira
Alvarado, Maria Teresa
Cruz, Claudia
Méndez, Claudia
author_facet Correa-Cárdenas, Camilo A.
Pérez, Julie
Patino, Luz H.
Ramírez, Juan David
Duque, Maria Clara
Romero, Yanira
Cantillo-Barraza, Omar
Rodríguez, Omaira
Alvarado, Maria Teresa
Cruz, Claudia
Méndez, Claudia
author_sort Correa-Cárdenas, Camilo A.
collection PubMed
description BACKGROUND: Leishmaniasis is one of the most important infectious diseases affecting the Colombian National Army due to the high number of reported cases and exposure throughout military operations in endemic areas. The main aim of this study was to estimate the geographical distribution along with the genetic diversity and treatment outcome of Leishmania species in Colombian military personnel. METHODS: Skin lesion samples by smear and aspirate were collected in 136 patients having parasitological cutaneous leishmaniasis (CL) diagnosis. DNA was extracted, the nuclear marker heat shock protein 70 (HSP70) was amplified by PCR and sequenced. Leishmania species were identified by BLASTn. The geo-spatial distribution of the identified parasites was determined according to the possible site of infection. Gene tree was constructed by maximum likelihood (ML), diversity indices (π, h) were estimated and haplotype network was constructed under the Templeton-Crandall-Sing algorithm in order to determine the geographic relationships of the genetic variants of Leishmania species circulating in Colombian military population. RESULTS: The species were identified in 77.94% of the samples, with a predominance of L. braziliensis (65.09%), followed by L. panamensis (31.13%), L. naiffi by the first time reported in Colombia in two patients (1.89%) as well as L. lindenbergi in a single patient (0.945%) with possible infection in the municipality of Miraflores, Guaviare and L. infantum in a single patient (0.945%) notified with CL in the municipality of Tumaco, Nariño. The phylogenetic analysis was consistent according to bootstrap, showing four strongly differentiated clades. CONCLUSIONS: The geo-spatial distribution suggested that L. braziliensis has a greater abundance, while L. panamensis has a greater dispersion. The phylogenetic relationships of Leishmania species in Colombian military personnel was estimated with the confirmation of two new species circulating without prior report in the country and a species with no background for CL in the Colombian army. A substantial genetic diversity of Leishmania braziliensis was defined. This study contributes through the understanding of the molecular epidemiology to the CL transmission in Colombia. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12879-020-05529-y.
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spelling pubmed-77248852020-12-09 Distribution, treatment outcome and genetic diversity of Leishmania species in military personnel from Colombia with cutaneous leishmaniasis Correa-Cárdenas, Camilo A. Pérez, Julie Patino, Luz H. Ramírez, Juan David Duque, Maria Clara Romero, Yanira Cantillo-Barraza, Omar Rodríguez, Omaira Alvarado, Maria Teresa Cruz, Claudia Méndez, Claudia BMC Infect Dis Research Article BACKGROUND: Leishmaniasis is one of the most important infectious diseases affecting the Colombian National Army due to the high number of reported cases and exposure throughout military operations in endemic areas. The main aim of this study was to estimate the geographical distribution along with the genetic diversity and treatment outcome of Leishmania species in Colombian military personnel. METHODS: Skin lesion samples by smear and aspirate were collected in 136 patients having parasitological cutaneous leishmaniasis (CL) diagnosis. DNA was extracted, the nuclear marker heat shock protein 70 (HSP70) was amplified by PCR and sequenced. Leishmania species were identified by BLASTn. The geo-spatial distribution of the identified parasites was determined according to the possible site of infection. Gene tree was constructed by maximum likelihood (ML), diversity indices (π, h) were estimated and haplotype network was constructed under the Templeton-Crandall-Sing algorithm in order to determine the geographic relationships of the genetic variants of Leishmania species circulating in Colombian military population. RESULTS: The species were identified in 77.94% of the samples, with a predominance of L. braziliensis (65.09%), followed by L. panamensis (31.13%), L. naiffi by the first time reported in Colombia in two patients (1.89%) as well as L. lindenbergi in a single patient (0.945%) with possible infection in the municipality of Miraflores, Guaviare and L. infantum in a single patient (0.945%) notified with CL in the municipality of Tumaco, Nariño. The phylogenetic analysis was consistent according to bootstrap, showing four strongly differentiated clades. CONCLUSIONS: The geo-spatial distribution suggested that L. braziliensis has a greater abundance, while L. panamensis has a greater dispersion. The phylogenetic relationships of Leishmania species in Colombian military personnel was estimated with the confirmation of two new species circulating without prior report in the country and a species with no background for CL in the Colombian army. A substantial genetic diversity of Leishmania braziliensis was defined. This study contributes through the understanding of the molecular epidemiology to the CL transmission in Colombia. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12879-020-05529-y. BioMed Central 2020-12-09 /pmc/articles/PMC7724885/ /pubmed/33297972 http://dx.doi.org/10.1186/s12879-020-05529-y Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research Article
Correa-Cárdenas, Camilo A.
Pérez, Julie
Patino, Luz H.
Ramírez, Juan David
Duque, Maria Clara
Romero, Yanira
Cantillo-Barraza, Omar
Rodríguez, Omaira
Alvarado, Maria Teresa
Cruz, Claudia
Méndez, Claudia
Distribution, treatment outcome and genetic diversity of Leishmania species in military personnel from Colombia with cutaneous leishmaniasis
title Distribution, treatment outcome and genetic diversity of Leishmania species in military personnel from Colombia with cutaneous leishmaniasis
title_full Distribution, treatment outcome and genetic diversity of Leishmania species in military personnel from Colombia with cutaneous leishmaniasis
title_fullStr Distribution, treatment outcome and genetic diversity of Leishmania species in military personnel from Colombia with cutaneous leishmaniasis
title_full_unstemmed Distribution, treatment outcome and genetic diversity of Leishmania species in military personnel from Colombia with cutaneous leishmaniasis
title_short Distribution, treatment outcome and genetic diversity of Leishmania species in military personnel from Colombia with cutaneous leishmaniasis
title_sort distribution, treatment outcome and genetic diversity of leishmania species in military personnel from colombia with cutaneous leishmaniasis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7724885/
https://www.ncbi.nlm.nih.gov/pubmed/33297972
http://dx.doi.org/10.1186/s12879-020-05529-y
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