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Mini-III RNase-based dual-color system for in vivo mRNA tracking

Mini-III RNase (mR3), a member of RNase III endonuclease family, can bind to and cleave double-stranded RNAs (dsRNAs). Inactive mR3 protein without the α5β-α6 loop loses the dsRNA cleavage activity, but retains dsRNA binding activity. Here, we establish an inactive mR3-based non-engineered mR3/dsRNA...

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Detalles Bibliográficos
Autores principales:	Zhang, Lin, Chen, Luxi, Chen, Jing, Shen, Weimin, Meng, Anming
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	The Company of Biologists Ltd 2020
Materias:	Techniques and Resources
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7725608/ https://www.ncbi.nlm.nih.gov/pubmed/33093152 http://dx.doi.org/10.1242/dev.190728

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