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Transcriptome analysis of wheat spikes in response to Tilletia controversa Kühn which cause wheat dwarf bunt

Wheat dwarf bunt is caused by Tilletia controversa Kühn, which is one of the most destructive diseases of wheat worldwide. To explore the interaction of T. controversa and wheat, we analysed the transcriptome profile of spikes of the susceptible wheat cultivar Dongxuan 3, which was subjected to a T....

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Autores principales: Ren, Zhaoyu, Liu, Jianjian, Din, Ghulam Muhae Ud, Zhang, Han, Du, Zhenzhen, Chen, Wanquan, Liu, Taiguo, Zhang, Jianmin, Zhao, Sifeng, Gao, Li
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7725808/
https://www.ncbi.nlm.nih.gov/pubmed/33299089
http://dx.doi.org/10.1038/s41598-020-78628-0
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author Ren, Zhaoyu
Liu, Jianjian
Din, Ghulam Muhae Ud
Zhang, Han
Du, Zhenzhen
Chen, Wanquan
Liu, Taiguo
Zhang, Jianmin
Zhao, Sifeng
Gao, Li
author_facet Ren, Zhaoyu
Liu, Jianjian
Din, Ghulam Muhae Ud
Zhang, Han
Du, Zhenzhen
Chen, Wanquan
Liu, Taiguo
Zhang, Jianmin
Zhao, Sifeng
Gao, Li
author_sort Ren, Zhaoyu
collection PubMed
description Wheat dwarf bunt is caused by Tilletia controversa Kühn, which is one of the most destructive diseases of wheat worldwide. To explore the interaction of T. controversa and wheat, we analysed the transcriptome profile of spikes of the susceptible wheat cultivar Dongxuan 3, which was subjected to a T. controversa infection and a mock infection. The results obtained from a differential expression analysis of T. controversa-infected plants compared with mock-infected ones showed that 10,867 out of 21,354 genes were upregulated, while 10,487 genes were downregulated, and these genes were enriched in 205 different pathways. Our findings demonstrated that the genes associated with defence against diseases, such as PR-related genes, WRKY transcription factors and mitogen-activated protein kinase genes, were more highly expressed in response to T. controversa infection. Additionally, a number of genes related to physiological attributes were expressed during infection. Three pathways were differentiated based on the characteristics of gene ontology classification. KEGG enrichment analysis showed that twenty genes were expressed differentially during the infection of wheat with T. controversa. Notable changes were observed in the transcriptomes of wheat plants after infection. The results of this study may help to elucidate the mechanism governing the interactions between this pathogen and wheat plants and may facilitate the development of new methods to increase the resistance level of wheat against T. controversa, including the overexpression of defence-related genes.
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spelling pubmed-77258082020-12-14 Transcriptome analysis of wheat spikes in response to Tilletia controversa Kühn which cause wheat dwarf bunt Ren, Zhaoyu Liu, Jianjian Din, Ghulam Muhae Ud Zhang, Han Du, Zhenzhen Chen, Wanquan Liu, Taiguo Zhang, Jianmin Zhao, Sifeng Gao, Li Sci Rep Article Wheat dwarf bunt is caused by Tilletia controversa Kühn, which is one of the most destructive diseases of wheat worldwide. To explore the interaction of T. controversa and wheat, we analysed the transcriptome profile of spikes of the susceptible wheat cultivar Dongxuan 3, which was subjected to a T. controversa infection and a mock infection. The results obtained from a differential expression analysis of T. controversa-infected plants compared with mock-infected ones showed that 10,867 out of 21,354 genes were upregulated, while 10,487 genes were downregulated, and these genes were enriched in 205 different pathways. Our findings demonstrated that the genes associated with defence against diseases, such as PR-related genes, WRKY transcription factors and mitogen-activated protein kinase genes, were more highly expressed in response to T. controversa infection. Additionally, a number of genes related to physiological attributes were expressed during infection. Three pathways were differentiated based on the characteristics of gene ontology classification. KEGG enrichment analysis showed that twenty genes were expressed differentially during the infection of wheat with T. controversa. Notable changes were observed in the transcriptomes of wheat plants after infection. The results of this study may help to elucidate the mechanism governing the interactions between this pathogen and wheat plants and may facilitate the development of new methods to increase the resistance level of wheat against T. controversa, including the overexpression of defence-related genes. Nature Publishing Group UK 2020-12-09 /pmc/articles/PMC7725808/ /pubmed/33299089 http://dx.doi.org/10.1038/s41598-020-78628-0 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Ren, Zhaoyu
Liu, Jianjian
Din, Ghulam Muhae Ud
Zhang, Han
Du, Zhenzhen
Chen, Wanquan
Liu, Taiguo
Zhang, Jianmin
Zhao, Sifeng
Gao, Li
Transcriptome analysis of wheat spikes in response to Tilletia controversa Kühn which cause wheat dwarf bunt
title Transcriptome analysis of wheat spikes in response to Tilletia controversa Kühn which cause wheat dwarf bunt
title_full Transcriptome analysis of wheat spikes in response to Tilletia controversa Kühn which cause wheat dwarf bunt
title_fullStr Transcriptome analysis of wheat spikes in response to Tilletia controversa Kühn which cause wheat dwarf bunt
title_full_unstemmed Transcriptome analysis of wheat spikes in response to Tilletia controversa Kühn which cause wheat dwarf bunt
title_short Transcriptome analysis of wheat spikes in response to Tilletia controversa Kühn which cause wheat dwarf bunt
title_sort transcriptome analysis of wheat spikes in response to tilletia controversa kühn which cause wheat dwarf bunt
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7725808/
https://www.ncbi.nlm.nih.gov/pubmed/33299089
http://dx.doi.org/10.1038/s41598-020-78628-0
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