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Extracellular Vesicles Derived From Colorectal Cancer Affects CD8 T Cells: An Analysis Based on Body Mass Index

Colorectal cancer (CRC) is one of the most widely diagnosed cancers worldwide. It has been shown that the body-mass index (BMI) of the patients could influence the tumor microenvironment, treatment response, and overall survival rates. Nevertheless, the mechanism on how BMI affects the tumorigenesis...

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Autores principales: Abu, Nadiah, Othman, Norahayu, Ab Razak, Nur’ Syahada, Bakarurraini, Nurul Ainaa’ Adilah Rus, Nasir, Siti Nurmi, Soh, Joanne Ern Chi, Mazlan, Luqman, Azman, Zairul Azwan Mohd, Jamal, Rahman
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7726136/
https://www.ncbi.nlm.nih.gov/pubmed/33324632
http://dx.doi.org/10.3389/fcell.2020.564648
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author Abu, Nadiah
Othman, Norahayu
Ab Razak, Nur’ Syahada
Bakarurraini, Nurul Ainaa’ Adilah Rus
Nasir, Siti Nurmi
Soh, Joanne Ern Chi
Mazlan, Luqman
Azman, Zairul Azwan Mohd
Jamal, Rahman
author_facet Abu, Nadiah
Othman, Norahayu
Ab Razak, Nur’ Syahada
Bakarurraini, Nurul Ainaa’ Adilah Rus
Nasir, Siti Nurmi
Soh, Joanne Ern Chi
Mazlan, Luqman
Azman, Zairul Azwan Mohd
Jamal, Rahman
author_sort Abu, Nadiah
collection PubMed
description Colorectal cancer (CRC) is one of the most widely diagnosed cancers worldwide. It has been shown that the body-mass index (BMI) of the patients could influence the tumor microenvironment, treatment response, and overall survival rates. Nevertheless, the mechanism on how BMI affects the tumorigenesis process, particularly the tumor microenvironment is still elusive. Herein, we postulate that extracellular vesicles (EVs) from CRC patients and non-CRC volunteers with different BMI could affect immune cells differently, in CD8 T cells particularly. We isolated the EVs from the archived serum of CRC patients with high and low BMI, as well as healthy controls with similar BMI status. The EVs were further characterized via electron microscopy, western blot and dynamic light scattering. Then, functional analysis was performed on CD8 T cells including apoptosis, cell proliferation, gene expression profiling and cytokine release upon co-incubation with the different EVs. Our results suggest that CRC-derived EVs were able to regulate the CD8 T cells. In some assays, low BMI EVs were functionally different than high BMI EVs. This study highlights the possible difference in the regulatory mechanism of cancer patients-derived EVs, especially on CD8 T cells.
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spelling pubmed-77261362020-12-14 Extracellular Vesicles Derived From Colorectal Cancer Affects CD8 T Cells: An Analysis Based on Body Mass Index Abu, Nadiah Othman, Norahayu Ab Razak, Nur’ Syahada Bakarurraini, Nurul Ainaa’ Adilah Rus Nasir, Siti Nurmi Soh, Joanne Ern Chi Mazlan, Luqman Azman, Zairul Azwan Mohd Jamal, Rahman Front Cell Dev Biol Cell and Developmental Biology Colorectal cancer (CRC) is one of the most widely diagnosed cancers worldwide. It has been shown that the body-mass index (BMI) of the patients could influence the tumor microenvironment, treatment response, and overall survival rates. Nevertheless, the mechanism on how BMI affects the tumorigenesis process, particularly the tumor microenvironment is still elusive. Herein, we postulate that extracellular vesicles (EVs) from CRC patients and non-CRC volunteers with different BMI could affect immune cells differently, in CD8 T cells particularly. We isolated the EVs from the archived serum of CRC patients with high and low BMI, as well as healthy controls with similar BMI status. The EVs were further characterized via electron microscopy, western blot and dynamic light scattering. Then, functional analysis was performed on CD8 T cells including apoptosis, cell proliferation, gene expression profiling and cytokine release upon co-incubation with the different EVs. Our results suggest that CRC-derived EVs were able to regulate the CD8 T cells. In some assays, low BMI EVs were functionally different than high BMI EVs. This study highlights the possible difference in the regulatory mechanism of cancer patients-derived EVs, especially on CD8 T cells. Frontiers Media S.A. 2020-11-26 /pmc/articles/PMC7726136/ /pubmed/33324632 http://dx.doi.org/10.3389/fcell.2020.564648 Text en Copyright © 2020 Abu, Othman, Ab Razak, Bakarurraini, Nasir, Soh, Mazlan, Azman and Jamal. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cell and Developmental Biology
Abu, Nadiah
Othman, Norahayu
Ab Razak, Nur’ Syahada
Bakarurraini, Nurul Ainaa’ Adilah Rus
Nasir, Siti Nurmi
Soh, Joanne Ern Chi
Mazlan, Luqman
Azman, Zairul Azwan Mohd
Jamal, Rahman
Extracellular Vesicles Derived From Colorectal Cancer Affects CD8 T Cells: An Analysis Based on Body Mass Index
title Extracellular Vesicles Derived From Colorectal Cancer Affects CD8 T Cells: An Analysis Based on Body Mass Index
title_full Extracellular Vesicles Derived From Colorectal Cancer Affects CD8 T Cells: An Analysis Based on Body Mass Index
title_fullStr Extracellular Vesicles Derived From Colorectal Cancer Affects CD8 T Cells: An Analysis Based on Body Mass Index
title_full_unstemmed Extracellular Vesicles Derived From Colorectal Cancer Affects CD8 T Cells: An Analysis Based on Body Mass Index
title_short Extracellular Vesicles Derived From Colorectal Cancer Affects CD8 T Cells: An Analysis Based on Body Mass Index
title_sort extracellular vesicles derived from colorectal cancer affects cd8 t cells: an analysis based on body mass index
topic Cell and Developmental Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7726136/
https://www.ncbi.nlm.nih.gov/pubmed/33324632
http://dx.doi.org/10.3389/fcell.2020.564648
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