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6S-1 RNA Contributes to Sporulation and Parasporal Crystal Formation in Bacillus thuringiensis
6S RNA is a kind of high-abundance non-coding RNA that globally regulates bacterial transcription by interacting with RNA polymerase holoenzyme. Through bioinformatics analysis, we found that there are two tandem 6S RNA-encoding genes in the genomes of Bacillus cereus group bacteria. Using Bacillus...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2020
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7726162/ https://www.ncbi.nlm.nih.gov/pubmed/33324388 http://dx.doi.org/10.3389/fmicb.2020.604458 |
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author | Li, Zhou Zhu, Li Yu, Zhaoqing Liu, Lu Chou, Shan-Ho Wang, Jieping He, Jin |
author_facet | Li, Zhou Zhu, Li Yu, Zhaoqing Liu, Lu Chou, Shan-Ho Wang, Jieping He, Jin |
author_sort | Li, Zhou |
collection | PubMed |
description | 6S RNA is a kind of high-abundance non-coding RNA that globally regulates bacterial transcription by interacting with RNA polymerase holoenzyme. Through bioinformatics analysis, we found that there are two tandem 6S RNA-encoding genes in the genomes of Bacillus cereus group bacteria. Using Bacillus thuringiensis BMB171 as the starting strain, we have explored the physiological functions of 6S RNAs, and found that the genes ssrSA and ssrSB encoding 6S-1 and 6S-2 RNAs were located in the same operon and are co-transcribed as a precursor that might be processed by specific ribonucleases to form mature 6S-1 and 6S-2 RNAs. We also constructed two single-gene deletion mutant strains ΔssrSA and ΔssrSB and a double-gene deletion mutant strain ΔssrSAB by means of the markerless gene knockout method. Our data show that deletion of 6S-1 RNA inhibited the growth of B. thuringiensis in the stationary phase, leading to lysis of some bacterial cells. Furthermore, deletion of 6S-1 RNA also significantly reduced the spore number and parasporal crystal content. Our work reveals that B. thuringiensis 6S RNA played an important regulatory role in ensuring the sporulation and parasporal crystal formation. |
format | Online Article Text |
id | pubmed-7726162 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-77261622020-12-14 6S-1 RNA Contributes to Sporulation and Parasporal Crystal Formation in Bacillus thuringiensis Li, Zhou Zhu, Li Yu, Zhaoqing Liu, Lu Chou, Shan-Ho Wang, Jieping He, Jin Front Microbiol Microbiology 6S RNA is a kind of high-abundance non-coding RNA that globally regulates bacterial transcription by interacting with RNA polymerase holoenzyme. Through bioinformatics analysis, we found that there are two tandem 6S RNA-encoding genes in the genomes of Bacillus cereus group bacteria. Using Bacillus thuringiensis BMB171 as the starting strain, we have explored the physiological functions of 6S RNAs, and found that the genes ssrSA and ssrSB encoding 6S-1 and 6S-2 RNAs were located in the same operon and are co-transcribed as a precursor that might be processed by specific ribonucleases to form mature 6S-1 and 6S-2 RNAs. We also constructed two single-gene deletion mutant strains ΔssrSA and ΔssrSB and a double-gene deletion mutant strain ΔssrSAB by means of the markerless gene knockout method. Our data show that deletion of 6S-1 RNA inhibited the growth of B. thuringiensis in the stationary phase, leading to lysis of some bacterial cells. Furthermore, deletion of 6S-1 RNA also significantly reduced the spore number and parasporal crystal content. Our work reveals that B. thuringiensis 6S RNA played an important regulatory role in ensuring the sporulation and parasporal crystal formation. Frontiers Media S.A. 2020-11-26 /pmc/articles/PMC7726162/ /pubmed/33324388 http://dx.doi.org/10.3389/fmicb.2020.604458 Text en Copyright © 2020 Li, Zhu, Yu, Liu, Chou, Wang and He. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Li, Zhou Zhu, Li Yu, Zhaoqing Liu, Lu Chou, Shan-Ho Wang, Jieping He, Jin 6S-1 RNA Contributes to Sporulation and Parasporal Crystal Formation in Bacillus thuringiensis |
title | 6S-1 RNA Contributes to Sporulation and Parasporal Crystal Formation in Bacillus thuringiensis |
title_full | 6S-1 RNA Contributes to Sporulation and Parasporal Crystal Formation in Bacillus thuringiensis |
title_fullStr | 6S-1 RNA Contributes to Sporulation and Parasporal Crystal Formation in Bacillus thuringiensis |
title_full_unstemmed | 6S-1 RNA Contributes to Sporulation and Parasporal Crystal Formation in Bacillus thuringiensis |
title_short | 6S-1 RNA Contributes to Sporulation and Parasporal Crystal Formation in Bacillus thuringiensis |
title_sort | 6s-1 rna contributes to sporulation and parasporal crystal formation in bacillus thuringiensis |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7726162/ https://www.ncbi.nlm.nih.gov/pubmed/33324388 http://dx.doi.org/10.3389/fmicb.2020.604458 |
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