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Relative contributions of various endogenous and exogenous factors to the mosquito microbiota
BACKGROUND: The commensal microbiota of mosquitoes impacts their development, immunity, and competency, and could provide a target for alternative entomological control approaches. However, despite the importance of the mosquito/microbiota interactions, little is known about the relative contributio...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7726613/ https://www.ncbi.nlm.nih.gov/pubmed/33303025 http://dx.doi.org/10.1186/s13071-020-04491-7 |
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author | Bogale, Haikel N. Cannon, Matthew V. Keita, Kalil Camara, Denka Barry, Yaya Keita, Moussa Coulibaly, Drissa Kone, Abdoulaye K. Doumbo, Ogobara K. Thera, Mahamadou A. Plowe, Christopher V. Travassos, Mark Irish, Seth Serre, David |
author_facet | Bogale, Haikel N. Cannon, Matthew V. Keita, Kalil Camara, Denka Barry, Yaya Keita, Moussa Coulibaly, Drissa Kone, Abdoulaye K. Doumbo, Ogobara K. Thera, Mahamadou A. Plowe, Christopher V. Travassos, Mark Irish, Seth Serre, David |
author_sort | Bogale, Haikel N. |
collection | PubMed |
description | BACKGROUND: The commensal microbiota of mosquitoes impacts their development, immunity, and competency, and could provide a target for alternative entomological control approaches. However, despite the importance of the mosquito/microbiota interactions, little is known about the relative contribution of endogenous and exogenous factors in shaping the bacterial communities of mosquitoes. METHODS: We used a high-throughput sequencing-based assay to characterize the bacterial composition and diversity of 665 individual field-caught mosquitoes, as well as their species, genotype at an insecticide resistance locus, blood-meal composition, and the eukaryotic parasites and viruses they carry. We then used these data to rigorously estimate the individual effect of each parameter on the bacterial diversity as well as the relative contribution of each parameter to the microbial composition. RESULTS: Overall, multivariate analyses did not reveal any significant contribution of the mosquito species, insecticide resistance, or blood meal to the bacterial composition of the mosquitoes surveyed, and infection with parasites and viruses only contributed very marginally. The main driver of the bacterial diversity was the location at which each mosquito was collected, which explained roughly 20% of the variance observed. CONCLUSIONS: This analysis shows that when confounding factors are taken into account, the site at which the mosquitoes are collected is the main driver of the bacterial diversity of wild-caught mosquitoes, although further studies will be needed to determine which specific components of the local environment affect bacterial composition. GRAPHIC ABSTRACT: [Image: see text] |
format | Online Article Text |
id | pubmed-7726613 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-77266132020-12-10 Relative contributions of various endogenous and exogenous factors to the mosquito microbiota Bogale, Haikel N. Cannon, Matthew V. Keita, Kalil Camara, Denka Barry, Yaya Keita, Moussa Coulibaly, Drissa Kone, Abdoulaye K. Doumbo, Ogobara K. Thera, Mahamadou A. Plowe, Christopher V. Travassos, Mark Irish, Seth Serre, David Parasit Vectors Research BACKGROUND: The commensal microbiota of mosquitoes impacts their development, immunity, and competency, and could provide a target for alternative entomological control approaches. However, despite the importance of the mosquito/microbiota interactions, little is known about the relative contribution of endogenous and exogenous factors in shaping the bacterial communities of mosquitoes. METHODS: We used a high-throughput sequencing-based assay to characterize the bacterial composition and diversity of 665 individual field-caught mosquitoes, as well as their species, genotype at an insecticide resistance locus, blood-meal composition, and the eukaryotic parasites and viruses they carry. We then used these data to rigorously estimate the individual effect of each parameter on the bacterial diversity as well as the relative contribution of each parameter to the microbial composition. RESULTS: Overall, multivariate analyses did not reveal any significant contribution of the mosquito species, insecticide resistance, or blood meal to the bacterial composition of the mosquitoes surveyed, and infection with parasites and viruses only contributed very marginally. The main driver of the bacterial diversity was the location at which each mosquito was collected, which explained roughly 20% of the variance observed. CONCLUSIONS: This analysis shows that when confounding factors are taken into account, the site at which the mosquitoes are collected is the main driver of the bacterial diversity of wild-caught mosquitoes, although further studies will be needed to determine which specific components of the local environment affect bacterial composition. GRAPHIC ABSTRACT: [Image: see text] BioMed Central 2020-12-10 /pmc/articles/PMC7726613/ /pubmed/33303025 http://dx.doi.org/10.1186/s13071-020-04491-7 Text en © The Author(s) 2020 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Bogale, Haikel N. Cannon, Matthew V. Keita, Kalil Camara, Denka Barry, Yaya Keita, Moussa Coulibaly, Drissa Kone, Abdoulaye K. Doumbo, Ogobara K. Thera, Mahamadou A. Plowe, Christopher V. Travassos, Mark Irish, Seth Serre, David Relative contributions of various endogenous and exogenous factors to the mosquito microbiota |
title | Relative contributions of various endogenous and exogenous factors to the mosquito microbiota |
title_full | Relative contributions of various endogenous and exogenous factors to the mosquito microbiota |
title_fullStr | Relative contributions of various endogenous and exogenous factors to the mosquito microbiota |
title_full_unstemmed | Relative contributions of various endogenous and exogenous factors to the mosquito microbiota |
title_short | Relative contributions of various endogenous and exogenous factors to the mosquito microbiota |
title_sort | relative contributions of various endogenous and exogenous factors to the mosquito microbiota |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7726613/ https://www.ncbi.nlm.nih.gov/pubmed/33303025 http://dx.doi.org/10.1186/s13071-020-04491-7 |
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