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In vitro virucidal activity of povidone iodine gargle and mouthwash against SARS-CoV-2: implications for dental practice
Introduction Virus particles in respiratory droplets and aerosols generated during medical/dental procedures are a potential source of SARS-CoV-2 cross infection. In the dental setting, oral decontamination could be an important adjunct to personal protective equipment and is recommended by a number...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Nature Publishing Group UK
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7726738/ https://www.ncbi.nlm.nih.gov/pubmed/33303923 http://dx.doi.org/10.1038/s41415-020-2402-0 |
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author | Hassandarvish, Pouya Tiong, Vunjia Mohamed, Nurul Azmawati Arumugam, Harsha Ananthanarayanan, Abhishek Qasuri, Murtaza Hadjiat, Yacine Abubakar, Sazaly |
author_facet | Hassandarvish, Pouya Tiong, Vunjia Mohamed, Nurul Azmawati Arumugam, Harsha Ananthanarayanan, Abhishek Qasuri, Murtaza Hadjiat, Yacine Abubakar, Sazaly |
author_sort | Hassandarvish, Pouya |
collection | PubMed |
description | Introduction Virus particles in respiratory droplets and aerosols generated during medical/dental procedures are a potential source of SARS-CoV-2 cross infection. In the dental setting, oral decontamination could be an important adjunct to personal protective equipment and is recommended by a number of national COVID-19 guidance documents for dental settings. Aim To assess the in vitrovirucidal activity of an oral povidone iodine (PVP-I) product against SARS-CoV-2. Material and methods BETADINE gargle and mouthwash (1% PVP-I) was tested against SARS-CoV-2 virus under both clean and dirty conditions using a suspension assay based on EN14476 methodology. Virucidal activity of the product, undiluted and at 1:2 dilution, was tested at contact times of 15, 30 and 60 seconds. Viral titres were calculated using the Spearman-Kärber method and reported as median tissue culture infectious dose (TCID(50)/ml). Results The undiluted product achieved >5 log10 reduction in viral titres compared to the control at 15, 30 and 60 seconds under both clean and dirty conditions. At a twofold dilution (0.5% PVP-I), the test product demonstrated >4 log10 kill at 15 seconds and >5 log10 kill at 30 and 60 seconds in both clean and dirty conditions. Conclusion PVP-I gargle and mouthwash product, undiluted and at 1:2 dilution, demonstrated potent and rapid virucidal activity (≥4 log10 reduction of viral titre) in 15 seconds against SARS-CoV-2 in vitro. The PVP-I gargle and mouthwash product is widely available and could be readily integrated into infection control measures during dental treatment including pre-procedural oral decontamination. |
format | Online Article Text |
id | pubmed-7726738 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-77267382020-12-10 In vitro virucidal activity of povidone iodine gargle and mouthwash against SARS-CoV-2: implications for dental practice Hassandarvish, Pouya Tiong, Vunjia Mohamed, Nurul Azmawati Arumugam, Harsha Ananthanarayanan, Abhishek Qasuri, Murtaza Hadjiat, Yacine Abubakar, Sazaly Br Dent J Research Introduction Virus particles in respiratory droplets and aerosols generated during medical/dental procedures are a potential source of SARS-CoV-2 cross infection. In the dental setting, oral decontamination could be an important adjunct to personal protective equipment and is recommended by a number of national COVID-19 guidance documents for dental settings. Aim To assess the in vitrovirucidal activity of an oral povidone iodine (PVP-I) product against SARS-CoV-2. Material and methods BETADINE gargle and mouthwash (1% PVP-I) was tested against SARS-CoV-2 virus under both clean and dirty conditions using a suspension assay based on EN14476 methodology. Virucidal activity of the product, undiluted and at 1:2 dilution, was tested at contact times of 15, 30 and 60 seconds. Viral titres were calculated using the Spearman-Kärber method and reported as median tissue culture infectious dose (TCID(50)/ml). Results The undiluted product achieved >5 log10 reduction in viral titres compared to the control at 15, 30 and 60 seconds under both clean and dirty conditions. At a twofold dilution (0.5% PVP-I), the test product demonstrated >4 log10 kill at 15 seconds and >5 log10 kill at 30 and 60 seconds in both clean and dirty conditions. Conclusion PVP-I gargle and mouthwash product, undiluted and at 1:2 dilution, demonstrated potent and rapid virucidal activity (≥4 log10 reduction of viral titre) in 15 seconds against SARS-CoV-2 in vitro. The PVP-I gargle and mouthwash product is widely available and could be readily integrated into infection control measures during dental treatment including pre-procedural oral decontamination. Nature Publishing Group UK 2020-12-10 /pmc/articles/PMC7726738/ /pubmed/33303923 http://dx.doi.org/10.1038/s41415-020-2402-0 Text en © British Dental Association 2020 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic. |
spellingShingle | Research Hassandarvish, Pouya Tiong, Vunjia Mohamed, Nurul Azmawati Arumugam, Harsha Ananthanarayanan, Abhishek Qasuri, Murtaza Hadjiat, Yacine Abubakar, Sazaly In vitro virucidal activity of povidone iodine gargle and mouthwash against SARS-CoV-2: implications for dental practice |
title | In vitro virucidal activity of povidone iodine gargle and mouthwash against SARS-CoV-2: implications for dental practice |
title_full | In vitro virucidal activity of povidone iodine gargle and mouthwash against SARS-CoV-2: implications for dental practice |
title_fullStr | In vitro virucidal activity of povidone iodine gargle and mouthwash against SARS-CoV-2: implications for dental practice |
title_full_unstemmed | In vitro virucidal activity of povidone iodine gargle and mouthwash against SARS-CoV-2: implications for dental practice |
title_short | In vitro virucidal activity of povidone iodine gargle and mouthwash against SARS-CoV-2: implications for dental practice |
title_sort | in vitro virucidal activity of povidone iodine gargle and mouthwash against sars-cov-2: implications for dental practice |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7726738/ https://www.ncbi.nlm.nih.gov/pubmed/33303923 http://dx.doi.org/10.1038/s41415-020-2402-0 |
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