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Phenotypic and molecular screenings for determination of cassava mosaic disease (CMD) status in farmers’ fields in Ebonyi State, Nigeria
African cassava mosaic virus (ACMV) and East African cassava mosaic virus (EACMV) are among the major constraints to cassava productivity within tropical and sub-tropical regions, including Ebonyi State, Nigeria. Thus, virus indexing has become imperative to determine the status of cassava mosaic di...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Springer Netherlands
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7727096/ https://www.ncbi.nlm.nih.gov/pubmed/33301051 http://dx.doi.org/10.1007/s11033-020-06039-5 |
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author | Igwe, David Okeh Anyanwu, Chidinma Blessing Afiukwa, Celestine Azubuike Nnamani, Catherine Veronica Nweke, Friday Nwalo Ude, George Nkem Ubi, Benjamin Ewa |
author_facet | Igwe, David Okeh Anyanwu, Chidinma Blessing Afiukwa, Celestine Azubuike Nnamani, Catherine Veronica Nweke, Friday Nwalo Ude, George Nkem Ubi, Benjamin Ewa |
author_sort | Igwe, David Okeh |
collection | PubMed |
description | African cassava mosaic virus (ACMV) and East African cassava mosaic virus (EACMV) are among the major constraints to cassava productivity within tropical and sub-tropical regions, including Ebonyi State, Nigeria. Thus, virus indexing has become imperative to determine the status of cassava mosaic disease (CMD) in Ebonyi State, to implement appropriate preventive and control strategies. Seventy-eight cassava accessions obtained from different locations of Ebonyi State were phenotypically scored, using scales 1–5 depending on CMD symptomatic expressions, followed by multiplex-PCR and sequencing for validation. 11% of cassava accessions cultivated in Afikpo were resistant (RE) to ACMV compared to 8% of moderately-resistant (MR) accessions in Izzi and 55% of tolerant (TO) ACMV accessions in Ebonyi. 100% of cassava accessions in Onicha and 66% in Afikpo South were susceptible (SU) and highly susceptible (HS) to ACMV, respectively. With multiplex-PCR, 97.4% (ACMV) and 2.6% (EACMV) were positive. Dunn’s multiple comparison tests of CMD mean incidence demonstrated differences (P < 0.05), except between RE and MR, and TO and MR. More transitions (A/G, C/T) compared to transversions (A/T, G/T), were detected, with nonsynonymous mutations (Leucine/Isoleucine; Valine/Isoleucine; Arginine/Lysine; Methionine/Isoleucine), and good bit-scores (91.13–99.07% identites; e-values of 7.00e(−148)–0.00e(+00)). Phylogeny resolved the sequences into five major groups. DNA sequencing validated the detected ACMV and EACMV species. This study revealed variants of ACMV and low adoption of RE and MR cassava accessions in the farmers’ fields. The findings will guide in getting disease-free and resistant varieties as planting materials to significantly mitigate the CMD spread in Ebonyi State, Nigeria. SUPPLEMENTARY INFORMATION: The online version of this article (10.1007/s11033-020-06039-5) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-7727096 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Springer Netherlands |
record_format | MEDLINE/PubMed |
spelling | pubmed-77270962020-12-10 Phenotypic and molecular screenings for determination of cassava mosaic disease (CMD) status in farmers’ fields in Ebonyi State, Nigeria Igwe, David Okeh Anyanwu, Chidinma Blessing Afiukwa, Celestine Azubuike Nnamani, Catherine Veronica Nweke, Friday Nwalo Ude, George Nkem Ubi, Benjamin Ewa Mol Biol Rep Original Article African cassava mosaic virus (ACMV) and East African cassava mosaic virus (EACMV) are among the major constraints to cassava productivity within tropical and sub-tropical regions, including Ebonyi State, Nigeria. Thus, virus indexing has become imperative to determine the status of cassava mosaic disease (CMD) in Ebonyi State, to implement appropriate preventive and control strategies. Seventy-eight cassava accessions obtained from different locations of Ebonyi State were phenotypically scored, using scales 1–5 depending on CMD symptomatic expressions, followed by multiplex-PCR and sequencing for validation. 11% of cassava accessions cultivated in Afikpo were resistant (RE) to ACMV compared to 8% of moderately-resistant (MR) accessions in Izzi and 55% of tolerant (TO) ACMV accessions in Ebonyi. 100% of cassava accessions in Onicha and 66% in Afikpo South were susceptible (SU) and highly susceptible (HS) to ACMV, respectively. With multiplex-PCR, 97.4% (ACMV) and 2.6% (EACMV) were positive. Dunn’s multiple comparison tests of CMD mean incidence demonstrated differences (P < 0.05), except between RE and MR, and TO and MR. More transitions (A/G, C/T) compared to transversions (A/T, G/T), were detected, with nonsynonymous mutations (Leucine/Isoleucine; Valine/Isoleucine; Arginine/Lysine; Methionine/Isoleucine), and good bit-scores (91.13–99.07% identites; e-values of 7.00e(−148)–0.00e(+00)). Phylogeny resolved the sequences into five major groups. DNA sequencing validated the detected ACMV and EACMV species. This study revealed variants of ACMV and low adoption of RE and MR cassava accessions in the farmers’ fields. The findings will guide in getting disease-free and resistant varieties as planting materials to significantly mitigate the CMD spread in Ebonyi State, Nigeria. SUPPLEMENTARY INFORMATION: The online version of this article (10.1007/s11033-020-06039-5) contains supplementary material, which is available to authorized users. Springer Netherlands 2020-12-10 2021 /pmc/articles/PMC7727096/ /pubmed/33301051 http://dx.doi.org/10.1007/s11033-020-06039-5 Text en © The Author(s), under exclusive licence to Springer Nature B.V. part of Springer Nature 2020 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic. |
spellingShingle | Original Article Igwe, David Okeh Anyanwu, Chidinma Blessing Afiukwa, Celestine Azubuike Nnamani, Catherine Veronica Nweke, Friday Nwalo Ude, George Nkem Ubi, Benjamin Ewa Phenotypic and molecular screenings for determination of cassava mosaic disease (CMD) status in farmers’ fields in Ebonyi State, Nigeria |
title | Phenotypic and molecular screenings for determination of cassava mosaic disease (CMD) status in farmers’ fields in Ebonyi State, Nigeria |
title_full | Phenotypic and molecular screenings for determination of cassava mosaic disease (CMD) status in farmers’ fields in Ebonyi State, Nigeria |
title_fullStr | Phenotypic and molecular screenings for determination of cassava mosaic disease (CMD) status in farmers’ fields in Ebonyi State, Nigeria |
title_full_unstemmed | Phenotypic and molecular screenings for determination of cassava mosaic disease (CMD) status in farmers’ fields in Ebonyi State, Nigeria |
title_short | Phenotypic and molecular screenings for determination of cassava mosaic disease (CMD) status in farmers’ fields in Ebonyi State, Nigeria |
title_sort | phenotypic and molecular screenings for determination of cassava mosaic disease (cmd) status in farmers’ fields in ebonyi state, nigeria |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7727096/ https://www.ncbi.nlm.nih.gov/pubmed/33301051 http://dx.doi.org/10.1007/s11033-020-06039-5 |
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