Cargando…

Air sampling for detection of infectious laryngotracheitis (ILT) in commercial poultry flocks

OBJECTIVE: Infectious laryngotracheitis (ILT) is an acute and highly contagious viral respiratory disease of poultry, caused by gallid herpesvirus 1 (ILTV), which causes significant economic losses. Due to recent outbreaks of ILT in Australia, it has been proposed that ILT could be transmitted betwe...

Descripción completa

Detalles Bibliográficos
Autores principales: Brown, Lauren, Premaratna, Dilhani, Segal, Yonatan, Beddoe, Travis
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7727125/
https://www.ncbi.nlm.nih.gov/pubmed/33298179
http://dx.doi.org/10.1186/s13104-020-05399-2
_version_ 1783621035769200640
author Brown, Lauren
Premaratna, Dilhani
Segal, Yonatan
Beddoe, Travis
author_facet Brown, Lauren
Premaratna, Dilhani
Segal, Yonatan
Beddoe, Travis
author_sort Brown, Lauren
collection PubMed
description OBJECTIVE: Infectious laryngotracheitis (ILT) is an acute and highly contagious viral respiratory disease of poultry, caused by gallid herpesvirus 1 (ILTV), which causes significant economic losses. Due to recent outbreaks of ILT in Australia, it has been proposed that ILT could be transmitted between poultry sheds by airborne transmission; however, there has never been direct detection of ILTV from air samples. We aimed to optimize a sampling system for the detection of airborne ILTV in poultry sheds. RESULTS: Poultry farms with a known outbreaks of ILT were used for detection of airborne ILTV. Infected chickens were verified by detection of ILTV nucleic acid in feather shafts with all farms being positive. Using a liquid cyclonic impinging device, it was found that recovery and detection of airborne ILTV was possible in alkaline PEG buffer. Additional sampling was performed at different heights to determine the presence of ILTV in the air. In farm 3, all three air samples at both heights were positive for ILTV while at farm 2 only one sample at 45 cm was positive. We envisaged in the future air sampling will be able to detect and track potential transmission of ILTV both inside and outside of the poultry shed.
format Online
Article
Text
id pubmed-7727125
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-77271252020-12-10 Air sampling for detection of infectious laryngotracheitis (ILT) in commercial poultry flocks Brown, Lauren Premaratna, Dilhani Segal, Yonatan Beddoe, Travis BMC Res Notes Research Note OBJECTIVE: Infectious laryngotracheitis (ILT) is an acute and highly contagious viral respiratory disease of poultry, caused by gallid herpesvirus 1 (ILTV), which causes significant economic losses. Due to recent outbreaks of ILT in Australia, it has been proposed that ILT could be transmitted between poultry sheds by airborne transmission; however, there has never been direct detection of ILTV from air samples. We aimed to optimize a sampling system for the detection of airborne ILTV in poultry sheds. RESULTS: Poultry farms with a known outbreaks of ILT were used for detection of airborne ILTV. Infected chickens were verified by detection of ILTV nucleic acid in feather shafts with all farms being positive. Using a liquid cyclonic impinging device, it was found that recovery and detection of airborne ILTV was possible in alkaline PEG buffer. Additional sampling was performed at different heights to determine the presence of ILTV in the air. In farm 3, all three air samples at both heights were positive for ILTV while at farm 2 only one sample at 45 cm was positive. We envisaged in the future air sampling will be able to detect and track potential transmission of ILTV both inside and outside of the poultry shed. BioMed Central 2020-12-09 /pmc/articles/PMC7727125/ /pubmed/33298179 http://dx.doi.org/10.1186/s13104-020-05399-2 Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research Note
Brown, Lauren
Premaratna, Dilhani
Segal, Yonatan
Beddoe, Travis
Air sampling for detection of infectious laryngotracheitis (ILT) in commercial poultry flocks
title Air sampling for detection of infectious laryngotracheitis (ILT) in commercial poultry flocks
title_full Air sampling for detection of infectious laryngotracheitis (ILT) in commercial poultry flocks
title_fullStr Air sampling for detection of infectious laryngotracheitis (ILT) in commercial poultry flocks
title_full_unstemmed Air sampling for detection of infectious laryngotracheitis (ILT) in commercial poultry flocks
title_short Air sampling for detection of infectious laryngotracheitis (ILT) in commercial poultry flocks
title_sort air sampling for detection of infectious laryngotracheitis (ilt) in commercial poultry flocks
topic Research Note
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7727125/
https://www.ncbi.nlm.nih.gov/pubmed/33298179
http://dx.doi.org/10.1186/s13104-020-05399-2
work_keys_str_mv AT brownlauren airsamplingfordetectionofinfectiouslaryngotracheitisiltincommercialpoultryflocks
AT premaratnadilhani airsamplingfordetectionofinfectiouslaryngotracheitisiltincommercialpoultryflocks
AT segalyonatan airsamplingfordetectionofinfectiouslaryngotracheitisiltincommercialpoultryflocks
AT beddoetravis airsamplingfordetectionofinfectiouslaryngotracheitisiltincommercialpoultryflocks